Protein detail

EHD1

EH domain-containing protein 1 (PAST homolog 1) (hPAST1) (Testilin)

Entry name EHD1	UniProt ID Q9H4M9	EVMP confidence score 0.88
Supporting publications (n) 114	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

EH domain-containing protein 1 (PAST homolog 1) (hPAST1) (Testilin)

Protein Class (3)

Plasma proteinsPredicted intracellular proteinsTransporters

Protein Function (2)

Transporters:Transporter channels and pores
Predicted intracellular proteins

Ensembl

ENSG00000110047

Entrez Gene Symbol

EHD1

Gene Synonym (5)

FLJ42622FLJ44618H-PASTHPAST1PAST1

Gene Description

EH domain containing 1

Chromosome

Position

64851642-64888296

Supporting publications (n)

114

EVMP confidence score

0.88

Fluorescence & Localization2

Cell SpecificAstrocytes

Function & Pathway6

Protein Function (2)

Transporters:Transporter channels and pores
Predicted intracellular proteins

Cellular Component (15)

Page 1 of 2

Molecular Function (7)

KEGG

hsa04144 Endocytosis

Reactome (2)

Mediation Categories (2)

Fusion and delivery mediationImmune mediation

Relations & Evidence11

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
EHD1	EGFR	P00533	Y	453	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (6)

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Protein Complex Composition (3)

3 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ARL8B CCT6A CCT8 CLTC CYP24A1 EHD1 GFPT2 GK IPO9 MTARC2 PPP2R1A QNG1 RPN1 SLC25A11 SLC27A4	O94808 P04843 P30153 P32189 P40227 P50990 Q00610 Q02978 Q07973 Q5T6V5 Q6P1M0 Q969Z3 Q96P70 Q9H4M9 Q9NVJ2	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4692
ANXA6 EHD1 PAFAH1B2 PAFAH1B3 SMAD1 TBCB	P08133 P68402 Q15102 Q15797 Q99426 Q9H4M9	0:0:0:0:0:0	hu.MAP2
ACTMAP EHD1	Q5BKX5 Q9H4M9	0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryWestern blotting	2	37886648 38343172

Sequence, Structure & Domains13

Sequences

Length

534

Mass

60,627

Sequence

MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE

3D Structural Models

Turn

523..525

Helix

440..443; 445..454; 464..473; 478..488; 498..512; 528..530

Beta Strand

458..463; 494..497

3D Structure

NMR spectroscopy (5)

Domain & Motif Annotations

Coiled Coil

198..227

Domain (CC)

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.

Domain (FT)

55..286; Dynamin-type G; 444..532; EH; 476..511; EF-hand

Region

65..72; G1 motif; 91..92; G2 motif; 153..156; G3 motif; 219..222; G4 motif; 243; G5 motif

Protein Families (3)

TRAFAC class dynamin-like GTPase superfamily
Dynamin/Fzo/YdjA family
EHD subfamily

Sequence Similarities

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.

Clinical Relevance4

Antibody (4)

HPA049890 HPA049986 HPA066751 HPA067747

Interaction Protein (5)

ENSG00000013016ENSG00000099940ENSG00000100139ENSG00000131381ENSG00000185722

Interaction Count

Interaction Dataset (2)

intact_biogridintact_biogrid_bioplex

Supporting Publications113

PMID	Title	Abstract
38225453	Deep proteomic analysis of obstetric antiphospholipid syndrome by DIA-MS of extracellular vesicle enriched fractions.	No abstract available
38441169	Identifying a core protein signature of small extracellular vesicles derived from B-cell precursor acute lymphoblastic leukaemia.	No abstract available
38490958	Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer.	No abstract available
38556629	Differential effects of physiological agonists on the proteome of platelet-derived extracellular vesicles.	No abstract available
38564289	Proteomic profiles of peritoneal fluid-derived small extracellular vesicles correlate with patient outcome in ovarian cancer.	No abstract available
38612515	Proteomic Analysis of Salivary Extracellular Vesicles from COVID-19 Patients Reveals a Specific Anti-COVID-19 Response Protein Signature.	No abstract available
38731868	The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer.	No abstract available
38937789	The RNA binding protein IGF2BP2/IMP2 alters the cargo of cancer cell-derived extracellular vesicles supporting tumor-associated macrophages.	No abstract available
38938674	Gestational age at birth influences protein and RNA content in human milk extracellular vesicles.	No abstract available
39001700	Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles.	No abstract available

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