Protein detail

EKI1

Ethanolamine kinase 1 (EKI 1) (EC 2.7.1.82)

Entry name EKI1	UniProt ID Q9HBU6	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification Cancer-related genesEnzymesHuman disease related genesMetabolic proteinsPredicted intracellular proteinsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Ethanolamine kinase 1 (EKI 1) (EC 2.7.1.82)

Protein Class (6)

Cancer-related genesEnzymesHuman disease related genesMetabolic proteinsPredicted intracellular proteinsPredicted membrane proteins

Protein Function (5)

Predicted intracellular proteins
ENZYME proteins:Transferases
Enzymes
Human disease related genes:Cancers:Cancers of haematopoietic and lymphoid tissues
Cancer-related genes

Ensembl

ENSG00000139163

Entrez Gene Symbol

ETNK1

Gene Synonym (2)

EKIEKI1

Gene Description

Ethanolamine kinase 1

Chromosome

Position

22625075-22690665

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization5

Tissue Specificlymphoid tissueCell SpecificcDCSingle-Nuclei Brain Specificcentral nervous system macrophageBlood Cell Specificclassical monocyteBlood Lineage SpecificB-cells

Function & Pathway8

Protein Function (5)

Predicted intracellular proteins
ENZYME proteins:Transferases
Enzymes
Human disease related genes:Cancers:Cancers of haematopoietic and lymphoid tissues
Cancer-related genes

Cellular Component (3)

Molecular Function (3)

Biological Process (3)

KEGG (2)

Reactome (4)

Canonical Pathways (2)

M257 Pid ephrinb rev pathway
M62 Pid ephb fwd pathway

Mediation Categories

Metabolism mediation

Relations & Evidence14

Ligand-Receptor Signaling (12)

12 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
transmembrane_sosui	transmembrane_predicted	Almen2009	No	No	No	No	No
transmembrane_tmhmm	transmembrane_predicted	Almen2009	No	No	No	No	No

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Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	BMPR1B ETNK1 EVA1C PIGA	O00238 P37287 P58658 Q9HBU6	0:0:0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Polymer Precipitation	Western blotting	1	38731868

Sequence, Structure & Domains9

Sequences

Length

452

Mass

50,968

Sequence

MLCGRPRSSSDNRNFLRERAGLSSAAVQTRIGNSAASRRSPAARPPVPAPPALPRGRPGTEGSTSLSAPAVLVVAVAVVVVVVSAVAWAMANYIHVPPGSPEVPKLNVTVQDQEEHRCREGALSLLQHLRPHWDPQEVTLQLFTDGITNKLIGCYVGNTMEDVVLVRIYGNKTELLVDRDEEVKSFRVLQAHGCAPQLYCTFNNGLCYEFIQGEALDPKHVCNPAIFRLIARQLAKIHAIHAHNGWIPKSNLWLKMGKYFSLIPTGFADEDINKRFLSDIPSSQILQEEMTWMKEILSNLGSPVVLCHNDLLCKNIIYNEKQGDVQFIDYEYSGYNYLAYDIGNHFNEFAGVSDVDYSLYPDRELQSQWLRAYLEAYKEFKGFGTEVTEKEVEILFIQVNQFALASHFFWGLWALIQAKYSTIEFDFLGYAIVRFNQYFKMKPEVTALKVPE

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9HBU6-1; Sequence=Displayed; Name=2; IsoId=Q9HBU6-2; Sequence=VSP_047191

Alternative Sequence

228..452; RLIARQLAKIHAIHAHNGWIPKSNLWLKMGKYFSLIPTGFADEDINKRFLSDIPSSQILQEEMTWMKEILSNLGSPVVLCHNDLLCKNIIYNEKQGDVQFIDYEYSGYNYLAYDIGNHFNEFAGVSDVDYSLYPDRELQSQWLRAYLEAYKEFKGFGTEVTEKEVEILFIQVNQFALASHFFWGLWALIQAKYSTIEFDFLGYAIVRFNQYFKMKPEVTALKVPE -> SLSSLTLCKGKTTRCFGLTGCRGSRLLLSFF (in isoform 2)

Domain & Motif Annotations

Compositional Bias

43..53; Pro residues

Region

26..64; Disordered

Protein Families

Choline/ethanolamine kinase family

Sequence Similarities

Belongs to the choline/ethanolamine kinase family.

Clinical Relevance2

Disease Involvement

Cancer-related genes

Antibody (2)

HPA010712 HPA029407

Supporting Publications1

PMID	Title	Abstract
22285593	Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes.	Notably, all isolations contained 40-100nm vesicles, and were positive for exosome markers (Alix, TSG101, HSP70) based on electron microscopy and Western blotting. Using the colorectal cancer cell line LIM1863 as a cell model, in this study we performed a comprehensive evaluation of current methods used for exosome isolation including ultracentrifugation (UC-Exos), OptiPrep™ density-based separation (DG-Exos), and immunoaffinity capture using anti-EpCAM coated magnetic beads (IAC-Exos).