Protein detail

ATG3

Ubiquitin-like-conjugating enzyme ATG3 (EC 2.3.2.-) (Autophagy-related protein 3) (APG3-like) (hApg3) (Protein PC3-96)

Entry name ATG3	UniProt ID Q9NT62	EVMP score 0.38
Frequency 1	Transmembrane count	Protein classification Predicted intracellular proteinsTransporters

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

Ubiquitin-like-conjugating enzyme ATG3 (EC 2.3.2.-) (Autophagy-related protein 3) (APG3-like) (hApg3) (Protein PC3-96)

Protein Class

Predicted intracellular proteinsTransporters

Protein Function

Transporters
Predicted intracellular proteins

Ensembl

ENSG00000144848

Entrez Gene Symbol

ATG3

Gene Synonym

APG3LDKFZp564M1178FLJ22125MGC15201PC3-96

Gene Description

Autophagy related 3

Chromosome

Position

112532510-112562046

Frequency

EVMP Score

0.38

Fluorescence & Localization

Cell SpecificDifferentiating spermatogoniaSingle-Nuclei Brain Specificvascular associated smooth muscle cell

Function & Pathway

Protein Function

Transporters
Predicted intracellular proteins

Cellular Component

Molecular Function

Biological Process

KEGG

Reactome

R-hsa-9612973 autophagy

Mediation Categories

Fusion and delivery mediationImmune mediation

Relations & Evidence

Enzyme-Mediated Modification

0 records.

Ligand-Receptor Signaling

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network

5 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
MLP3B	Q9GZQ8	ATG3	Q9NT62	Yes	Yes	No	SIGNORHPRDHINTIntActSPIKE_LCSPIKE	SIGNOR:22170151 HINT:35271311 SPIKE_LC:20562859 HINT:20562859 SPIKE_LC:17145710 HINT:18083104 IntAct:16303767 SPIKE:20562859 SPIKE:17145710 HPRD:16303767 IntAct:20562859
ATG3	Q9NT62	GBRAP	O95166	Yes	Yes	No	SIGNORHPRDHINTIntActSPIKE_LCSPIKE	HPRD:11825910 SPIKE:16303767 SPIKE_LC:16303767 SPIKE_LC:20562859 HINT:20562859 HINT:26043688 SPIKE_LC:17145710 IntAct:16303767 SPIKE:20562859 SPIKE:17145710 SIGNOR:16303767 HINT:37252361 HPRD:16303767 IntAct:20562859 HINT:16303767
ATG3	Q9NT62	GBRL2	P60520	Yes	Yes	No	SIGNORHPRDHINTIntActSPIKE_LCSPIKE	HINT:35271311 IntAct:17353931 SPIKE:16303767 SPIKE:17353931 SPIKE_LC:16303767 HINT:20562859 SPIKE_LC:17145710 SPIKE_LC:20562859 IntAct:16303767 SPIKE:20562859 SPIKE:17145710 SIGNOR:16303767 HPRD:16303767 SPIKE_LC:17353931 IntAct:20562859 HINT:17353931 HINT:16303767 HINT:33961781
MLP3C	Q9BXW4	ATG3	Q9NT62	Yes	Yes	No	SPIKESPIKE_LCSIGNOR	SIGNOR:22170151 SPIKE:20562859 SPIKE_LC:20562859
MLP3A	Q9H492	ATG3	Q9NT62	Yes	Yes	No	SIGNORHINTBioGRIDInnateDBSPIKE_LCSPIKE	SIGNOR:22170151 BioGRID:19838173 SPIKE:16303767 SPIKE_LC:16303767 SPIKE_LC:20562859 HINT:20562859 BioGRID:25503391 HINT:20061800 SPIKE:20562859 InnateDB:19838173 HINT:16303767

Protein Complex Composition

22 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	ATG3 TRIP10	Q15642 Q9NT62	0:0	hu.MAP
	ATG16L1 ATG3 ATG5	Q676U5 Q9H1Y0 Q9NT62	0:0:0	hu.MAP2

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Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	2	35119778 38716512

Sequence, Structure & Domains

Sequences

Length

314

Mass

35,864

Sequence

MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTYHNTGITGITEAVKEITLENKDNIRLQDCSALCEEEEDEDEGEAADMEEYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9NT62-1; Sequence=Displayed; Name=2; IsoId=Q9NT62-2; Sequence=VSP_013037

Alternative Sequence

290..314; LLIFLKFVQAVIPTIEYDYTRHFTM -> PSLYVRLVAKWLLTIFFLRNLV (in isoform 2)

3D Structural Models

Turn

28..31; 208..211; 242..244

Helix

36..49; 69..71; 157..163; 229..232; 233..235; 238..241; 267..278; 286..288; 289..298

Beta Strand

54..56; 72..79; 97..99; 101..105; 107..109; 199..207; 212..220; 245..248; 251..253; 258..261

3D Structure

NMR spectroscopy (1); X-ray crystallography (3)

Domain & Motif Annotations

Motif

4..19; Membrane-curvature-sensing motif; 101..103; Increases ATG3 translation efficiency by the ribosome assisted of EIF5A; 104..110; LIR motif; 166..169; Caspase cleavage motif LETD

Domain (CC)

The N-terminal region works in concert with its geometry-selective amphipathic helix (AH) to promote LC3-PE conjugation activity only on the target membrane.; DOMAIN: The LC3 interacting regions (LIR) motif mediates interaction with GABARAP and MAP1LC3A in a beta-sheet conformation-dependent manner (PubMed:37252361). The LIR motif is required for LC3 lipidation and ATG3~LC3 thioester formation (PubMed:37252361).; DOMAIN: The membrane-curvature-sensing motif targets curved membranes.

Region

140..170; Interaction with ATG12; 143..163; Disordered

Protein Families

ATG3 family

Sequence Similarities

Belongs to the ATG3 family.

Clinical Relevance

Antibody

CAB037260 HPA040471

Interaction Protein

ENSG00000034713ENSG00000057663ENSG00000139112ENSG00000140941ENSG00000145782ENSG00000170296

Interaction Count

Interaction Dataset

intact_biogrid_opencell_bioplexbiogrid_opencellintact_biogridintact_biogrid_opencell