Protein detail

SPHK1

Sphingosine kinase 1 (SK 1) (SPK 1) (EC 2.7.1.91) (Acetyltransferase SPHK1) (EC 2.3.1.-)

Entry name SPHK1	UniProt ID Q9NYA1	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count	Protein classification EnzymesMetabolic proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Sphingosine kinase 1 (SK 1) (SPK 1) (EC 2.7.1.91) (Acetyltransferase SPHK1) (EC 2.3.1.-)

Protein Class (3)

EnzymesMetabolic proteinsPredicted intracellular proteins

Protein Function (3)

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins

Ensembl

ENSG00000176170

Entrez Gene Symbol

SPHK1

Gene Synonym

SPHK

Gene Description

Sphingosine kinase 1

Chromosome

Position

76376584-76387860

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization6

Tissue SpecificintestineCell SpecificB-cellsSingle-Nuclei Brain Specificcentral nervous system macrophageBlood Cell Specificclassical monocyteBlood Lineage Specificdendritic cells

Function & Pathway7

Protein Function (3)

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins

Cellular Component (11)

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Molecular Function (12)

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Biological Process (3)

KEGG (10)

Reactome (15)

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Mediation Categories (5)

Clinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence37

Enzyme-Mediated Modification (12)

12 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
SPHK1	MAPK1	P28482	S	225	phosphorylation	SIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	SIGNOR:14532121 ProtMapper:14532121
SPHK1	MAPK1	P28482	S	311	phosphorylation	MIMPKEAPhosphoSite_MIMP	KEA:16522638
SPHK1	MAPK3	P27361	S	225	phosphorylation	SIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	SIGNOR:14532121 ProtMapper:14532121
SPHK1	MAPK3	P27361	S	311	phosphorylation	MIMPKEAPhosphoSite_MIMP	KEA:16522638
SPHK1	PPP2CA	P67775	S	225	dephosphorylation	DEPOD	DEPOD:18852266
SPHK1	PPP2CA	P67775	S	311	phosphorylation	KEA	KEA:16522638
SPHK1	PPP2CB	P62714	S	225	dephosphorylation	DEPOD	DEPOD:18852266
SPHK1	PRKCA	P17252	S	225	phosphorylation	Reactome_ProtMapperProtMapper
SPHK1	PRKCB	P05771	S	225	phosphorylation	Reactome_ProtMapperProtMapper
SPHK1	PRKCD	Q05655	S	225	phosphorylation	Reactome_ProtMapperProtMapper

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Ligand-Receptor Signaling (11)

11 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
transmembrane	transmembrane	GO_Intercell	No	No	No	No	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

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Regulatory Interaction Network (5)

5 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
KS6C1	Q96S38	SPHK1	Q9NYA1	Yes	Yes	No	HPRDPhosphoPointSIGNOR	SIGNOR:12077123 HPRD:12077123
E2AK2	P19525	SPHK1	Q9NYA1	Yes	Yes	No	REACH_ProtMapperSIGNORProtMapper	SIGNOR:32801355 ProtMapper:32801355
MK03	P27361	SPHK1	Q9NYA1	Yes	Yes	No	PhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperKinexus_KEAKEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:14532121 PhosphoSite:18852266 PhosphoSite:19830907 PhosphoSite:16522638 ProtMapper:14532121 PhosphoSite:21075214 PhosphoSite:15623571 SIGNOR:14532121 KEA:16522638 PhosphoSite:16243846 PhosphoSite:19914200
FHL2	Q14192	SPHK1	Q9NYA1	Yes	No	Yes	SPIKE_LCLit-BM-17SIGNOR	Lit-BM-17:16888242 SPIKE_LC:16888242 SIGNOR:16888242
MK01	P28482	SPHK1	Q9NYA1	Yes	Yes	No	PhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperKinexus_KEAKEASIGNOR_ProtMapperPhosphoSite_ProtMapper	SIGNOR:14532121 ProtMapper:14532121 KEA:16522638

Protein Complex Composition (8)

8 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
CDC5L CRNKL1 EFTUD2 PLRG1 PRPF8 SNRPA SNW1 SPHK1 SRP54 TSSC4 YJU2B	O43660 P09012 P13994 P61011 Q13573 Q15029 Q6P2Q9 Q99459 Q9BZJ0 Q9NYA1 Q9Y5U2	1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4601
BUD31 CDC5L CRNKL1 DNAJC17 EFTUD2 PAXBP1 PLRG1 PRPF8 RBM22 SNRPA SNW1 SPHK1 SRP54 TSSC4 XAB2	O43660 P09012 P41223 P61011 Q13573 Q15029 Q6P2Q9 Q99459 Q9BZJ0 Q9HCS7 Q9NVM6 Q9NW64 Q9NYA1 Q9Y5B6 Q9Y5U2	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4866
ARCN1 ARF4 PDE6D RHBDD3 SCPEP1 SFT2D1 SLC66A2 SNX10 SPHK1 TM9SF4 UBC ZDHHC13	O43924 P0CG48 P18085 P48444 Q8IUH4 Q8N2U9 Q8WV19 Q92544 Q9HB40 Q9NYA1 Q9Y3P4 Q9Y5X0	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6372
CCNA2 CCNE1 CDC6 CDK1 CDK2 CDK3 CKS1B CKS2 SPHK1 UBC	P06493 P0CG48 P20248 P24864 P24941 P33552 P61024 Q00526 Q99741 Q9NYA1	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6898
CKMT2 DCXR RAB33A SPHK1	P17540 Q14088 Q7Z4W1 Q9NYA1	0:0:0:0	hu.MAP
CYLD FGL2 MTFR1 SPATA2 SPHK1	Q14314 Q15390 Q9NQC7 Q9NYA1 Q9UM82	0:0:0:0:0	hu.MAP2
CYLD SPHK1	Q9NQC7 Q9NYA1	0:0	hu.MAP
SPHK1	Q9NYA1	3	PDB	PDB:3vzdPDB:3vzbPDB:3vzcPDB:4v24PDB:4l02

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometry	17	27086912 37211381 38113368 37322475 41068253 33592500 38014595 31617158 40730843 41307968 27821849 40840701 32230970 38716512 34576246 36982312 37438638

Sequence, Structure & Domains11

Sequences

Length

384

Mass

42,518

Sequence

MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q9NYA1-1; Sequence=Displayed; Name=2; IsoId=Q9NYA1-2; Sequence=VSP_035453; Name=3; IsoId=Q9NYA1-3; Sequence=VSP_047078

Alternative Sequence

1; M -> MSAQVLGFLRSWTPLPLAAPRGPAAAGNDAGAPAATAPGGEGEPHSRPCDARLGSTDKELKAGAAATGSAPTAPGTPWQREPRVEVM (in isoform 2); 3; P -> PVVGCGRGLFGFVFS (in isoform 3)

3D Structural Models

Turn

26..29

Helix

30..37; 39..44; 59..66; 69..71; 81..92; 97..100; 115..123; 131..144; 173..181; 182..190; 191..201; 215..218; 296..305; 306..308; 311..314

Beta Strand

13..21; 47..53; 73..80; 105..109; 146..157; 162..172; 206..214; 230..232; 249..251; 255..266; 285..291; 319..331; 333..335; 337..340; 343..346; 350..362

3D Structure

X-ray crystallography (5)

Domain & Motif Annotations

Motif

147..155; Nuclear export signal 1; 161..169; Nuclear export signal 2

Domain (FT)

12..159; DAGKc

Clinical Relevance4

Related Diseases (5)

Arterial occlusive disease Circulatory system disease Nasopharyngeal cancer Ovarian cancer Psychoactive substances use disorder

Biomarker

Terminated; Investigative; Phase 1/2; Phase 1

Drugs (4)

IDRONOXIL OPAGANIB DIMETHYLSPINGOSINE CHEMBL:CHEMBL554130

Antibody (2)

HPA022829 HPA028761

Supporting Publications1

PMID	Title	Abstract
32384937	Alzheimer's disease progression characterized by alterations in the molecular profiles and biogenesis of brain extracellular vesicles.	No abstract available