Protein detail

ITSN2

Intersectin-2 (SH3 domain-containing protein 1B) (SH3P18) (SH3P18-like WASP-associated protein)

Entry name ITSN2	UniProt ID Q9NZM3	EVMP confidence score 0.50
Supporting publications (n) 7	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Intersectin-2 (SH3 domain-containing protein 1B) (SH3P18) (SH3P18-like WASP-associated protein)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000198399

Entrez Gene Symbol

ITSN2

Gene Synonym (6)

KIAA1256PRO2015SH3D1BSH3P18SWASWAP

Gene Description

Intersectin 2

Chromosome

Position

24202864-24360536

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization6

Tissue SpecificliverCell SpecificcDCSingle-Nuclei Brain Specificcentral nervous system macrophageBlood Cell Specificclassical monocyteBlood Lineage Specificdendritic cells

Function & Pathway6

Protein Function

Predicted intracellular proteins

Cellular Component (7)

Molecular Function (4)

Biological Process (3)

Reactome (7)

Mediation Categories (3)

Adhesion and uptake mediationFusion and delivery mediationReceptor-signaling mediation

Relations & Evidence10

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
ITSN2	EGFR	P00533	Y	553	phosphorylation	BEL-Large-Corpus_ProtMapperProtMapper	ProtMapper:15951569

Ligand-Receptor Signaling (5)

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Protein Complex Composition (3)

3 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
CLIC3 ITSN2	O95833 Q9NZM3	0:0	hu.MAP
BCCIP CAPZB ITSN2 PTN	P21246 P47756 Q9NZM3 Q9P287	1:1:1:1	CompleatCFinder	Compleat:HC9836
ITSN2	Q9NZM3	2	PDB	PDB:4iio

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometryR Sequencing	5	38113368 37322475 33936570 40189497 38207106

Sequence, Structure & Domains13

Sequences

Length

1,697

Mass

193,461

Sequence

MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Alternative Products

Event=Alternative splicing; Named isoforms=4; Comment=Additional isoforms seem to exist.; Name=1; Synonyms=ITSN2-L; IsoId=Q9NZM3-1; Sequence=Displayed; Name=2; IsoId=Q9NZM3-2; Sequence=VSP_003892; Name=3; Synonyms=ITSN2-S1; IsoId=Q9NZM3-3; Sequence=VSP_003893, VSP_003894; Name=4; Synonyms=ITSN2-S2, SH3P18; IsoId=Q9NZM3-4; Sequence=VSP_003895

Alternative Sequence

622..648; Missing (in isoform 2); 1193..1697; Missing (in isoform 4); 1235..1249; FQKRMAESGFLTEGE -> RRLLLASSRGICCLS (in isoform 3); 1250..1697; Missing (in isoform 3)

3D Structural Models

Turn

1336..1340; 1627..1629

Helix

948..950; 1031..1033; 1207..1234; 1236..1242; 1247..1254; 1257..1272; 1288..1294; 1299..1321; 1323..1333; 1343..1346; 1349..1366; 1375..1393; 1655..1665

Beta Strand

762..766; 772..774; 783..786; 793..801; 804..809; 812..815; 818..821; 901..907; 924..931; 934..939; 942..947; 951..954; 983..987; 995..999; 1007..1010; 1012..1014; 1017..1020; 1027..1029; 1056..1060; 1079..1085; 1087..1095; 1104..1108; 1111..1114; 1129..1136; 1153..1156; 1161..1166; 1168..1171; 1180..1183; 1243..1245; 1569..1580; 1592..1598; 1601..1604; 1615..1625; 1631..1638; 1641..1644; 1647..1654; 1670..1673; 1675..1679; 1681..1690

3D Structure

NMR spectroscopy (5); X-ray crystallography (3)

Domain & Motif Annotations

Compositional Bias

219..230; Low complexity

Coiled Coil

361..756

Domain (FT)

22..110; EH 1; 54..89; EF-hand 1; 244..333; EH 2; 277..312; EF-hand 2; 757..818; SH3 1; 898..956; SH3 2; 981..1039; SH3 3; 1053..1117; SH3 4; 1127..1186; SH3 5; 1209..1395; DH; 1434..1544; PH; 1552..1668; C2

Region

219..241; Disordered; 350..378; Disordered; 691..752; Disordered

Clinical Relevance4

Antibody

HPA036475

Interaction Protein (2)

ENSG00000127527ENSG00000146648

Interaction Count

Interaction Dataset (2)

intact_biogrid_opencellintact_biogrid

Supporting Publications7

PMID	Title	Abstract
33592500	A Proteomic Approach to Understand the Clinical Significance of Acute Myeloid Leukemia-Derived Extracellular Vesicles Reflecting Essential Characteristics of Leukemia.	No abstract available
36146834	Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro.	No abstract available
37322475	Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration.	No abstract available
37926756	Extracellular vesicles from non-neuroendocrine SCLC cells promote adhesion and survival of neuroendocrine SCLC cells.	No abstract available
38113368	In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.	No abstract available
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available
40189497	Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.	No abstract available