Protein detail
EHD3
EH domain-containing protein 3 (PAST homolog 3)
Entry name EHD3 | UniProt ID | EVMP confidence score 0.53 |
Supporting publications (n) 1 | Transmembrane count | Protein classification Plasma proteinsPredicted intracellular proteins |
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.70High >= 0.60Medium >= 0.40Low >= 0.30Basic Information
Protein Names
EH domain-containing protein 3 (PAST homolog 3)
Protein Class (2)
Plasma proteinsPredicted intracellular proteins
Protein Function
Predicted intracellular proteins
Ensembl
Entrez Gene Symbol
Gene Synonym
PAST3
Gene Description
EH domain containing 3
Chromosome
2
Position
31234152-31269451
Supporting publications (n)
1
EVMP confidence score
0.53
Function & Pathway
Protein Function
Predicted intracellular proteins
Cellular Component (11)
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005769 early endosome
- GO:0005829 cytosol
- GO:0005886 plasma membrane
- GO:0005925 focal adhesion
- GO:0010008 endosome membrane
- GO:0020018 ciliary pocket membrane
- GO:0030139 endocytic vesicle
- GO:0048471 perinuclear region of cytoplasm
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Molecular Function (5)
Biological Process (3)
Reactome (2)
Canonical Pathways (2)
- M151 Pid ar tf pathway
- M58 Pid ar pathway
Mediation Categories (3)
Adhesion and uptake mediationFusion and delivery mediationImmune mediation
Relations & Evidence11
Ligand-Receptor Signaling (6)
6 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| intracellular | intracellular | ComPPI | |||||
| intracellular | intracellular | GO_Intercell | |||||
| intracellular | intracellular | UniProt_location | |||||
| intracellular | intracellular | OmniPath | |||||
| plasma_membrane | plasma_membrane | UniProt_location | |||||
| plasma_membrane | plasma_membrane | OmniPath |
Protein Complex Composition (4)
4 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| ACOT13EHD3HDDC3RBSNSSBP1STX16VPS45 | O14662Q04837Q8N4P3Q9H1K0Q9NPJ3Q9NRW7Q9NZN3 | 0:0:0:0:0:0:0 | hu.MAP2 | |||
| ACOT13EHD3HDDC3RBSNSTX16VPS45 | O14662Q8N4P3Q9H1K0Q9NPJ3Q9NRW7Q9NZN3 | 0:0:0:0:0:0 | hu.MAP2 | |||
| ACOT13EHD3RBSNSTX16VPS45 | O14662Q9H1K0Q9NPJ3Q9NRW7Q9NZN3 | 0:0:0:0:0 | hu.MAP2 | |||
| EHD3RBSNSTX16VPS45 | O14662Q9H1K0Q9NRW7Q9NZN3 | 0:0:0:0 | hu.MAP2 |
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography | Flow cytometry | 1 | 35611462 |
Sequence, Structure & Domains
Sequences
Length
535
Mass
60,887
Sequence
MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9NZN3-1; Sequence=Displayed; Name=2; IsoId=Q9NZN3-2; Sequence=VSP_056606, VSP_056607
Alternative Sequence
169..175; YDFAAVL -> PAAGPGL (in isoform 2); 176..535; Missing (in isoform 2)
Domain & Motif Annotations
Coiled Coil
198..227
Domain (CC)
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
Domain (FT)
55..286; Dynamin-type G; 444..532; EH; 476..511; EF-hand
Region
65..72; G1 motif; 91..92; G2 motif; 153..156; G3 motif; 219..222; G4 motif; 243; G5 motif
Protein Families (3)
- TRAFAC class dynamin-like GTPase superfamily
- Dynamin/Fzo/YdjA family
- EHD subfamily
Sequence Similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
Clinical Relevance
Supporting Publications1
| PMID | Title | Related sentences |
|---|---|---|
| 22285593 | Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. | Notably, all isolations contained 40-100nm vesicles, and were positive for exosome markers (Alix, TSG101, HSP70) based on electron microscopy and Western blotting. Using the colorectal cancer cell line LIM1863 as a cell model, in this study we performed a comprehensive evaluation of current methods used for exosome isolation including ultracentrifugation (UC-Exos), OptiPrep™ density-based separation (DG-Exos), and immunoaffinity capture using anti-EpCAM coated magnetic beads (IAC-Exos). |