Protein detail

EHD3

EH domain-containing protein 3 (PAST homolog 3)

Entry name EHD3	UniProt ID Q9NZN3	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

EH domain-containing protein 3 (PAST homolog 3)

Protein Class (2)

Plasma proteinsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000013016

Entrez Gene Symbol

EHD3

Gene Synonym

PAST3

Gene Description

EH domain containing 3

Chromosome

Position

31234152-31269451

Supporting publications (n)

EVMP confidence score

0.50

Function & Pathway8

Protein Function

Predicted intracellular proteins

Cellular Component (11)

Page 1 of 2

Molecular Function (5)

Biological Process (3)

KEGG

hsa04144 Endocytosis

Reactome (2)

Canonical Pathways (2)

M151 Pid ar tf pathway
M58 Pid ar pathway

Mediation Categories (3)

Adhesion and uptake mediationFusion and delivery mediationImmune mediation

Relations & Evidence11

Ligand-Receptor Signaling (6)

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Protein Complex Composition (4)

4 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database
ACOT13 EHD3 HDDC3 RBSN SSBP1 STX16 VPS45	O14662 Q04837 Q8N4P3 Q9H1K0 Q9NPJ3 Q9NRW7 Q9NZN3	0:0:0:0:0:0:0	hu.MAP2
ACOT13 EHD3 HDDC3 RBSN STX16 VPS45	O14662 Q8N4P3 Q9H1K0 Q9NPJ3 Q9NRW7 Q9NZN3	0:0:0:0:0:0	hu.MAP2
ACOT13 EHD3 RBSN STX16 VPS45	O14662 Q9H1K0 Q9NPJ3 Q9NRW7 Q9NZN3	0:0:0:0:0	hu.MAP2
EHD3 RBSN STX16 VPS45	O14662 Q9H1K0 Q9NRW7 Q9NZN3	0:0:0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Flow cytometry	1	35611462

Sequence, Structure & Domains11

Sequences

Length

535

Mass

60,887

Sequence

MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9NZN3-1; Sequence=Displayed; Name=2; IsoId=Q9NZN3-2; Sequence=VSP_056606, VSP_056607

Alternative Sequence

169..175; YDFAAVL -> PAAGPGL (in isoform 2); 176..535; Missing (in isoform 2)

Domain & Motif Annotations

Coiled Coil

198..227

Domain (CC)

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.

Domain (FT)

55..286; Dynamin-type G; 444..532; EH; 476..511; EF-hand

Region

65..72; G1 motif; 91..92; G2 motif; 153..156; G3 motif; 219..222; G4 motif; 243; G5 motif

Protein Families (3)

TRAFAC class dynamin-like GTPase superfamily
Dynamin/Fzo/YdjA family
EHD subfamily

Sequence Similarities

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.

Clinical Relevance4

Antibody (3)

HPA049890 HPA049986 HPA066751

Interaction Protein (3)

ENSG00000100139ENSG00000110047ENSG00000131381

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications1

PMID	Title	Abstract
22285593	Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes.	Notably, all isolations contained 40-100nm vesicles, and were positive for exosome markers (Alix, TSG101, HSP70) based on electron microscopy and Western blotting. Using the colorectal cancer cell line LIM1863 as a cell model, in this study we performed a comprehensive evaluation of current methods used for exosome isolation including ultracentrifugation (UC-Exos), OptiPrep™ density-based separation (DG-Exos), and immunoaffinity capture using anti-EpCAM coated magnetic beads (IAC-Exos).