Protein detail

EHD2

EH domain-containing protein 2 (PAST homolog 2)

Entry name EHD2	UniProt ID Q9NZN4	EVMP confidence score 0.50
Supporting publications (n) 5	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

EH domain-containing protein 2 (PAST homolog 2)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000024422

Entrez Gene Symbol

EHD2

Gene Synonym

PAST2

Gene Description

EH domain containing 2

Chromosome

Position

47713422-47743134

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization5

Tissue Specificskeletal muscleCell SpecificMyonucleiSingle-Nuclei Brain SpecificfibroblastBlood Cell SpecificneutrophilBlood Lineage Specificgranulocytes

Function & Pathway7

Protein Function

Predicted intracellular proteins

Cellular Component (14)

Page 1 of 2

Molecular Function (8)

Biological Process (3)

KEGG

hsa04144 Endocytosis

Reactome (2)

Mediation Categories (2)

Fusion and delivery mediationImmune mediation

Relations & Evidence10

Ligand-Receptor Signaling (6)

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Protein Complex Composition (3)

3 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database
CHEK1 CHRNA9 EHD2 NAT9 SLC30A7 TIMM29 TP53I13	O14757 Q8NBR0 Q8NEW0 Q9BSF4 Q9BTE0 Q9NZN4 Q9UGM1	0:0:0:0:0:0:0	hu.MAP2
CHEK1 EHD2 NAT9	O14757 Q9BTE0 Q9NZN4	0:0:0	hu.MAP2
DHX15 EHD2	O43143 Q9NZN4	0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient CentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometryWestern blottingR SequencingFlow cytometryMORPH	25	37862381 38441169 39949490 40928027 32795414 24769233 23585444 24505114 29242380 32295833 33709510 40619995 30975894 36635400 38490958 41201090 30760538 31320591 41216884 40091455 31588238 38136601 33114768 30451371 38564289

Sequence, Structure & Domains12

Sequences

Length

543

Mass

61,161

Sequence

MFSWLKRGGARGQQPEAIRTVTSALKELYRTKLLPLEEHYRFGAFHSPALEDADFDGKPMVLVAGQYSTGKTSFIQYLLEQEVPGSRVGPEPTTDCFVAVMHGDTEGTVPGNALVVDPDKPFRKLNPFGNTFLNRFMCAQLPNQVLESISIIDTPGILSGAKQRVSRGYDFPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGHEDKIRVVLNKADMVETQQLMRVYGALMWALGKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQDLFRDIQGLPRHAALRKLNDLVKRARLVRVHAYIISYLKKEMPSVFGKENKKKQLILKLPVIFAKIQLEHHISPGDFPDCQKMQELLMAHDFTKFHSLKPKLLEALDEMLTHDIAKLMPLLRQEELESTEVGVQGGAFEGTHMGPFVERGPDEAMEDGEEGSDDEAEWVVTKDKSKYDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIWKLSDVDRDGMLDDEEFALASHLIEAKLEGHGLPANLPRRLVPPSKRRHKGSAE

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9NZN4-1; Sequence=Displayed; Name=2; IsoId=Q9NZN4-2; Sequence=VSP_056212

Alternative Sequence

1..136; Missing (in isoform 2)

Domain & Motif Annotations

Compositional Bias

534..543; Basic residues

Motif

120..122; KPF loop; caveolar targeting

Domain (CC)

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.

Domain (FT)

55..286; Dynamin-type G; 449..537; EH; 481..516; EF-hand

Region

65..72; G1 motif; 91..92; G2 motif; 153..156; G3 motif; 219..222; G4 motif; 243; G5 motif; 320..340; Mediates membrane-binding; 523..543; Disordered

Protein Families (3)

TRAFAC class dynamin-like GTPase superfamily
Dynamin/Fzo/YdjA family
EHD subfamily

Sequence Similarities

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.

Clinical Relevance1

Antibody (4)

HPA047394 HPA049890 HPA049986 HPA066751

Supporting Publications5

PMID	Title	Abstract
31805958	Proteomic analysis of cerebrospinal fluid extracellular vesicles reveals synaptic injury, inflammation, and stress response markers in HIV patients with cognitive impairment.	No abstract available
37322475	Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration.	No abstract available
38113368	In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.	No abstract available
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available
40189497	Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.	No abstract available