Protein detail
ARHGC
Rho guanine nucleotide exchange factor 12 (Leukemia-associated RhoGEF)
Entry name ARHGC | UniProt ID | EVMP confidence score 0.63 |
Supporting publications (n) 6 | Transmembrane count | Protein classification Cancer-related genesDisease related genesPredicted intracellular proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
Rho guanine nucleotide exchange factor 12 (Leukemia-associated RhoGEF)
Protein Class (3)
Cancer-related genesDisease related genesPredicted intracellular proteins
Protein Function (3)
- Disease related genes
- Predicted intracellular proteins
- Cancer-related genes
Ensembl
Entrez Gene Symbol
Gene Synonym (2)
KIAA0382LARG
Gene Description
Rho guanine nucleotide exchange factor 12
Chromosome
11
Position
120336413-120489937
Supporting publications (n)
6
EVMP confidence score
0.63
Fluorescence & Localization2
Tissue SpecificepididymisCell SpecificEarly spermatids
Function & Pathway7
Protein Function (3)
- Disease related genes
- Predicted intracellular proteins
- Cancer-related genes
Cellular Component (4)
Molecular Function (4)
Biological Process (3)
KEGG (11)
- hsa04270 Vascular smooth muscle contraction
- KEGG:hsa04360 Axon guidance
- KEGG:hsa04611 Platelet activation
- KEGG:hsa04625 C-type lectin receptor signaling pathway
- KEGG:hsa04810 Regulation of actin cytoskeleton
- KEGG:hsa05130 Pathogenic Escherichia coli infection
- KEGG:hsa05135 Yersinia infection
- KEGG:hsa05152 Tuberculosis
- KEGG:hsa05163 Human cytomegalovirus infection
- KEGG:hsa05200 Pathways in cancer
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Reactome (16)
- R-hsa-9013148 cdc42 gtpase cycle
- R-hsa-204998 cell death signalling via nrage nrif and nade
- R-hsa-73887 death receptor signaling
- R-hsa-416482 g alpha 12 13 signalling events
- R-hsa-9675108 nervous system development
- R-hsa-193648 nrage signals death through jnk
- R-hsa-193704 p75 ntr receptor mediated signalling
- R-hsa-8980692 rhoa gtpase cycle
- R-hsa-9013026 rhob gtpase cycle
- R-hsa-9013106 rhoc gtpase cycle
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Mediation Categories
Receptor-signaling mediation
Relations & Evidence19
Enzyme-Mediated Modification (4)
4 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| ARHGEF12 | RPS6KA3 | P51812 | S | 1,288 | phosphorylation | REACH_ProtMapperSparser_ProtMapperProtMapper | ProtMapper:29279389 |
| ARHGEF12 | CDK1 | P06493 | S | 341 | phosphorylation | KEA | KEA:17570479 |
| ARHGEF12 | GSK3B | P49841 | S | 341 | phosphorylation | KEA | KEA:17570479 |
| ARHGEF12 | MAPK14 | Q16539 | S | 341 | phosphorylation | KEA | KEA:17570479 |
Ligand-Receptor Signaling (5)
5 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
Regulatory Interaction Network (5)
5 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| GNA13 | Q14344 | ARHGC | Q9NZN5 | Yes | Yes | No | KEGG-MEDICUSSIGNORHPRDBioGRIDWang | HPRD:12515866HPRD:11094164SIGNOR:12024019BioGRID:11094164 |
| FAK1 | Q05397 | ARHGC | Q9NZN5 | Yes | Yes | No | SIGNOR | SIGNOR:19273616 |
| GNA12 | Q03113 | ARHGC | Q9NZN5 | Yes | Yes | No | KEGG-MEDICUSHPRDWangSIGNOR | HPRD:12515866HPRD:11094164SIGNOR:12024019 |
| GNAQ | P50148 | ARHGC | Q9NZN5 | Yes | Yes | No | KEGG-MEDICUSSIGNOR | SIGNOR:12024019 |
| ARHGC | Q9NZN5 | RHOA | P61586 | Yes | Yes | No | WangAdhesomeKEGG-MEDICUSSIGNORHPRDHINTBioGRIDACSNIntActSPIKE_LCLit-BM-17 | Lit-BM-17:20300064SIGNOR:11094164SPIKE_LC:17145710Adhesome:23405219ACSN:11149925ACSN:9641915Adhesome:11373293HINT:15364580IntAct:20300064HINT:32203420SIGNOR:32203420HINT:20300064HINT:15331592Lit-BM-17:23405219Adhesome:20300064ACSN:9789025Adhesome:10592173Adhesome:15364580HPRD:11373293BioGRID:11373293HINT:11373293IntAct:15364580HINT:12515866HINT:33961781Adhesome:12515866ACSN:9113980HINT:34591642Lit-BM-17:11373293Lit-BM-17:15364580 |
Protein Complex Composition (4)
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationSize Exclusion Chromatography | Mass spectrometry | 1 | 29635386 |
Sequence, Structure & Domains13
Sequences
Length
1,544
Mass
173,232
Sequence
MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9NZN5-1; Sequence=Displayed; Name=2; IsoId=Q9NZN5-2; Sequence=VSP_008131
Alternative Sequence
48..66; Missing (in isoform 2)
3D Structural Models
Turn
122..124
Helix
102..106; 127..135; 769..772; 775..778; 783..812; 814..820; 825..832; 835..853; 865..872; 875..889; 891..904; 906..917; 919..921; 926..929; 932..949; 954..993; 1005..1007; 1008..1011; 1015..1017; 1082..1084; 1117..1137
Beta Strand
70..76; 85..87; 89..91; 93..96; 112..119; 137..145; 857..860; 1001..1003; 1020..1033; 1038..1053; 1056..1058; 1078..1081; 1085..1089; 1091..1093; 1096..1102; 1107..1113
3D Structure
NMR spectroscopy (2); X-ray crystallography (2)
Domain & Motif Annotations
Compositional Bias
28..45; Basic and acidic residues; 249..260; Polar residues; 293..309; Polar residues; 313..329; Basic and acidic residues; 330..344; Polar residues; 582..592; Basic and acidic residues; 631..640; Polar residues; 663..676; Low complexity; 1138..1149; Polar residues
Coiled Coil
194..262
Domain (FT)
72..151; PDZ; 367..558; RGSL; 787..977; DH; 1019..1132; PH
Region
1..62; Disordered; 247..346; Disordered; 570..706; Disordered; 1138..1179; Disordered
Clinical Relevance5
Disease Involvement (2)
Cancer-related genesProto-oncogene
Interaction Protein (8)
ENSG00000067560ENSG00000101558ENSG00000123159ENSG00000124164ENSG00000132694ENSG00000140443ENSG00000177189ENSG00000196586
Interaction Count
8
Interaction Dataset (3)
intact_biogridbiogrid_opencellintact_biogrid_opencell
Supporting Publications6
| PMID | Title | Abstract |
|---|---|---|
| 34265469 | Proteomic Landscape of Exosomes Reveals the Functional Contributions of CD151 in Triple-Negative Breast Cancer. | Furthermore, utilizing quantitative proteomics approach to reveal the proteomes of CD151-deleted exosomes and cells, we found that exosomal CD151 facilitated secretion of ribosomal proteins via exosomes while inhibiting exosome secretion of complement proteins. Moreover, we proved that CD151-deleted exosomes significantly decreased the migration and invasion of TNBC cells. Most importantly, we found that the tetraspanin CD151 expression levels in TNBC-derived serum exosomes were significantly higher than those exosomes from healthy subjects, and we validated our findings with samples from 16 additional donors. This is the first comparative study of the proteomes of TNBC patient-derived and CD151-deleted exosomes. |
| 38037300 | Proteomic profiling of paired human liver homogenate and tissue derived extracellular vesicles. | No abstract available |
| 38576002 | Therapy-induced senescent tumor cell-derived extracellular vesicles promote colorectal cancer progression through SERPINE1-mediated NF-κB p65 nuclear translocation. | No abstract available |
| 38871114 | Proteomic analysis of endothelial cells and extracellular vesicles in response to indoxyl sulfate: Mechanisms of endothelial dysfunction in chronic kidney disease. | No abstract available |
| 39996590 | Surface Double Dendritic Magnetic Microfibrils for Rapid Isolation and Proteomic Profiling of Extracellular Vesicles from Microliters of Biofluids. | No abstract available |
| 40689422 | Defining the Ovarian Cancer Precancerous Landscape through Modeling Fallopian Tube Epithelium Reprogramming Driven by Extracellular Vesicles. | No abstract available |