Protein detail

AKIB1

Ankyrin repeat and IBR domain-containing protein 1 (EC 2.3.2.31)

Entry name AKIB1	UniProt ID Q9P2G1	EVMP confidence score 0.38
Supporting publications (n) 3	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Ankyrin repeat and IBR domain-containing protein 1 (EC 2.3.2.31)

Protein Class (2)

EnzymesPredicted intracellular proteins

Protein Function (3)

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins

Ensembl

ENSG00000001629

Entrez Gene Symbol

ANKIB1

Gene Synonym (2)

DKFZP434A0225KIAA1386

Gene Description

Ankyrin repeat and IBR domain containing 1

Chromosome

Position

92245974-92401383

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization4

Tissue SpecificliverCell SpecificAdipocytesSingle-Nuclei Brain Specificcentral nervous system macrophageBlood Cell Specificeosinophil

Function & Pathway6

Protein Function (3)

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins

Cellular Component (2)

Molecular Function (4)

Biological Process (3)

Canonical Pathways

M81 Pid cdc42 pathway

Mediation Categories

Other mediation

Relations & Evidence145

Ligand-Receptor Signaling (3)

3 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (136)

136 records.

Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
COMPLEX:P0CG47_P61077	AKIB1	Q9P2G1	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P61088_P62979	AKIB1	Q9P2G1	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P51965_P62987	AKIB1	Q9P2G1	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P62979_Q13404	AKIB1	Q9P2G1	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P0CG48_Q9H832	AKIB1	Q9P2G1	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P62256_P62979	AKIB1	Q9P2G1	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:A0A1B0GUS4_P62979	AKIB1	Q9P2G1	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P61088_P62987	AKIB1	Q9P2G1	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P61077_P62979	AKIB1	Q9P2G1	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P62979_Q712K3	AKIB1	Q9P2G1	Yes	Yes	No	SIGNOR	SIGNOR:34199813

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Protein Complex Composition (5)

5 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
Ub:RBR_E3	ANKIB1 UBB	P0CG47 Q9P2G1	0:0	SIGNOR	SIGNOR:SIGNOR-C520
Ub:RBR_E3	ANKIB1 UBC	P0CG48 Q9P2G1	0:0	SIGNOR	SIGNOR:SIGNOR-C520
Ub:RBR_E3	ANKIB1 RPS27A	P62979 Q9P2G1	0:0	SIGNOR	SIGNOR:SIGNOR-C520
Ub:RBR_E3	ANKIB1 UBA52	P62987 Q9P2G1	0:0	SIGNOR	SIGNOR:SIGNOR-C520
	ANKIB1 ATAD5 CHTF18 ESCO2 GPI LIG1 PCNA RFC1 RFC2 RFC3 RFC4 RFC5 UBC	P06744 P0CG48 P12004 P18858 P35249 P35250 P35251 P40937 P40938 Q56NI9 Q8WVB6 Q96QE3 Q9P2G1	1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6063

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient CentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometry	9	35611462 32489530 37786918 34817906 28986585 27894104 30646616 39948040 38321535

Sequence, Structure & Domains12

Sequences

Length

1,089

Mass

122,002

Sequence

MGNTTTKFRKALINGDENLACQIYENNPQLKESLDPNTSYGEPYQHNTPLHYAARHGMNKILGTFLGRDGNPNKRNVHNETSMHLLCMGPQIMISEGALHPRLARPTEDDFRRADCLQMILKWKGAKLDQGEYERAAIDAVDNKKNTPLHYAAASGMKACVELLVKHGGDLFAENENKDTPCDCAEKQHHKDLALNLESQMVFSRDPEAEEIEAEYAALDKREPYEGLRPQDLRRLKDMLIVETADMLQAPLFTAEALLRAHDWDREKLLEAWMSNPENCCQRSGVQMPTPPPSGYNAWDTLPSPRTPRTTRSSVTSPDEISLSPGDLDTSLCDICMCSISVFEDPVDMPCGHDFCRGCWESFLNLKIQEGEAHNIFCPAYDCFQLVPVDIIESVVSKEMDKRYLQFDIKAFVENNPAIKWCPTPGCDRAVRLTKQGSNTSGSDTLSFPLLRAPAVDCGKGHLFCWECLGEAHEPCDCQTWKNWLQKITEMKPEELVGVSEAYEDAANCLWLLTNSKPCANCKSPIQKNEGCNHMQCAKCKYDFCWICLEEWKKHSSSTGGYYRCTRYEVIQHVEEQSKEMTVEAEKKHKRFQELDRFMHYYTRFKNHEHSYQLEQRLLKTAKEKMEQLSRALKETEGGCPDTTFIEDAVHVLLKTRRILKCSYPYGFFLEPKSTKKEIFELMQTDLEMVTEDLAQKVNRPYLRTPRHKIIKAACLVQQKRQEFLASVARGVAPADSPEAPRRSFAGGTWDWEYLGFASPEEYAEFQYRRRHRQRRRGDVHSLLSNPPDPDEPSESTLDIPEGGSSSRRPGTSVVSSASMSVLHSSSLRDYTPASRSENQDSLQALSSLDEDDPNILLAIQLSLQESGLALDEETRDFLSNEASLGAIGTSLPSRLDSVPRNTDSPRAALSSSELLELGDSLMRLGAENDPFSTDTLSSHPLSEARSDFCPSSSDPDSAGQDPNINDNLLGNIMAWFHDMNPQSIALIPPATTEISADSQLPCIKDGSEGVKDVELVLPEDSMFEDASVSEGRGTQIEENPLEENILAGEAASQAGDSGNEAANRGDGSDVSSQTPQTSSDWLEQVHLV

Domain & Motif Annotations

Compositional Bias

303..318; Low complexity; 931..941; Polar residues; 1070..1082; Polar residues

Repeat

45..74; ANK 1; 144..173; ANK 2

Coiled Coil

575..640

Zinc Finger

333..383; RING-type 1; 401..478; IBR-type; 519..548; RING-type 2; atypical

Domain (CC)

Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.

Domain (FT)

851..870; UIM

Region

281..321; Disordered; 329..569; TRIAD supradomain; 776..821; Disordered; 889..912; Disordered; 927..964; Disordered; 1026..1089; Disordered

Protein Families

RBR family

Sequence Similarities

Belongs to the RBR family.

Clinical Relevance1

Antibody (2)

HPA019358 HPA021780

Supporting Publications3

PMID	Title	Abstract
36146834	Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro.	No abstract available
38113368	In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.	No abstract available
40189497	Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.	No abstract available