Protein detail

LIMA1

LIM domain and actin-binding protein 1 (Epithelial protein lost in neoplasm)

Entry name LIMA1	UniProt ID Q9UHB6	EVMP confidence score 0.72
Supporting publications (n) 14	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

LIM domain and actin-binding protein 1 (Epithelial protein lost in neoplasm)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000050405

Entrez Gene Symbol

LIMA1

Gene Synonym

EPLIN

Gene Description

LIM domain and actin binding 1

Chromosome

Position

50175788-50283520

Supporting publications (n)

EVMP confidence score

0.72

Fluorescence & Localization1

Function & Pathway6

Protein Function

Predicted intracellular proteins

Cellular Component (8)

Molecular Function (5)

Biological Process (3)

KEGG

hsa04519 Cadherin signaling

Mediation Categories (3)

Adhesion and uptake mediationFusion and delivery mediationImmune mediation

Relations & Evidence18

Enzyme-Mediated Modification (8)

8 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
LIMA1	MAPK1	P28482	S	604	phosphorylation	SIGNOR	SIGNOR:23188829
LIMA1	MAPK1	P28482	S	362	phosphorylation	SIGNOR	SIGNOR:23188829
LIMA1	MAPK3	P27361	S	604	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
LIMA1	MAPK3	P27361	S	374	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
LIMA1	MAPK3	P27361	S	362	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
LIMA1	CAMK2D	Q13557	S	132	phosphorylation	PhosphoNetworks
LIMA1	CAMK2D	Q13557	S	369	phosphorylation	PhosphoNetworks
LIMA1	CAMK2D	Q13557	T	592	phosphorylation	PhosphoNetworks

Ligand-Receptor Signaling (7)

7 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Regulatory Interaction Network (2)

2 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
MK01	P28482	LIMA1	Q9UHB6	Yes	No	Yes	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	SIGNOR:23188829 ProtMapper:23188829
MK03	P27361	LIMA1	Q9UHB6	Yes	No	No	iPTMnetPhosphoSite_norefProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:28465438

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Size Exclusion Chromatography	Mass spectrometry	1	31414377

Sequence, Structure & Domains14

Sequences

Length

759

Mass

85,226

Sequence

MESSPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEETNMEKKRSNTENLSQHFRKGTLTVLKKKWENPGLGAESHTDSLRNSSTEIRHRADHPPAEVTSHAASGAKADQEEQIHPRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

Alternative Products

Event=Alternative promoter usage, Alternative splicing; Named isoforms=5; Name=Beta; IsoId=Q9UHB6-1; Sequence=Displayed; Name=Alpha; IsoId=Q9UHB6-2; Sequence=VSP_003116; Name=3; IsoId=Q9UHB6-3; Sequence=VSP_003117; Name=4; IsoId=Q9UHB6-4; Sequence=VSP_040136; Name=5; IsoId=Q9UHB6-5; Sequence=VSP_003116, VSP_040136

Alternative Sequence

1..302; Missing (in isoform 3); 1..160; Missing (in isoform Alpha and isoform 5); 344; R -> PG (in isoform 4 and isoform 5)

3D Structural Models

Turn

391..393; 412..414; 418..420; 426..428

Helix

439..445

Beta Strand

384..387; 401..404; 406..411; 432..434

3D Structure

NMR spectroscopy (1)

Domain & Motif Annotations

Compositional Bias

96..105; Basic and acidic residues; 146..177; Basic and acidic residues; 247..258; Basic and acidic residues; 362..376; Low complexity; 516..527; Basic and acidic residues; 556..567; Basic and acidic residues; 595..607; Polar residues; 644..655; Polar residues; 656..673; Basic and acidic residues; 674..691; Acidic residues; 693..709; Polar residues

Motif

164..166; Required for interaction with NPC1L1

Domain (CC)

Contains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than the N-terminus.

Domain (FT)

388..448; LIM zinc-binding

Region

78..131; Disordered; 146..182; Disordered; 211..264; Disordered; 323..381; Disordered; 493..513; Required for interaction with MYO5B; 509..709; Disordered

Clinical Relevance4

Antibody (2)

HPA023871 HPA052645

Interaction Protein (5)

ENSG00000077549ENSG00000119522ENSG00000122786ENSG00000164924ENSG00000196730

Interaction Count

Interaction Dataset (2)

biogrid_opencellintact_biogrid

Supporting Publications14

PMID	Title	Abstract
26826536	[Cardiovascular risk study in patients with renin-angiotensin system blockade by means of the proteone of circulating extracellular vesicles].	No abstract available
27821849	Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation.	No abstract available
30178811	Effect of exercise on the plasma vesicular proteome: a methodological study comparing acoustic trapping and centrifugation.	No abstract available
30915084	Extracellular Vesicles Mediate Mesenchymal Stromal Cell-Dependent Regulation of B Cell PI3K-AKT Signaling Pathway and Actin Cytoskeleton.	No abstract available
34064677	Ubiquinone Metabolism and Transcription HIF-1 Targets Pathway Are Toxicity Signature Pathways Present in Extracellular Vesicles of Paraquat-Exposed Human Brain Microvascular Endothelial Cells.	No abstract available
35119778	Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis.	No abstract available
36146834	Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro.	No abstract available
37926756	Extracellular vesicles from non-neuroendocrine SCLC cells promote adhesion and survival of neuroendocrine SCLC cells.	No abstract available
38113368	In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.	No abstract available
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available

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