Protein detail

NGAP

Ras GTPase-activating protein nGAP (RAS protein activator-like 2)

Entry name NGAP	UniProt ID Q9UJF2	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteinsRAS pathway related proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Ras GTPase-activating protein nGAP (RAS protein activator-like 2)

Protein Class (3)

Plasma proteinsPredicted intracellular proteinsRAS pathway related proteins

Protein Function (2)

Predicted intracellular proteins
RAS pathway related proteins

Ensembl

ENSG00000075391

Entrez Gene Symbol

RASAL2

Gene Synonym

nGAP

Gene Description

RAS protein activator like 2

Chromosome

Position

178094104-178484147

Supporting publications (n)

EVMP confidence score

0.38

Function & Pathway7

Protein Function (2)

Predicted intracellular proteins
RAS pathway related proteins

Cellular Component

GO:0005829 cytosol

Molecular Function (2)

Biological Process (3)

KEGG

hsa04014 Ras signaling pathway

Reactome (7)

Mediation Categories

Receptor-signaling mediation

Relations & Evidence9

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
RASAL2	PRKAA1	Q13131	S	351	phosphorylation	PhosphoSite

Ligand-Receptor Signaling (3)

3 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (1)

1 record.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
AAPK1	Q13131	NGAP	Q9UJF2	Yes	No	No	PhosphoSite	PhosphoSite:33563064

Protein Complex Composition (3)

3 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database
CSNK1A1 FAM83G FAM83H RASAL2 SPATA46 ZNF618	A6ND36 P48729 Q5T0L3 Q5T7W0 Q6ZRV2 Q9UJF2	0:0:0:0:0:0	hu.MAP2
CSNK1A1 FAM83G FAM83H RASAL2 ZNF618	A6ND36 P48729 Q5T7W0 Q6ZRV2 Q9UJF2	0:0:0:0:0	hu.MAP2
RASAL2 RFFL SHC1 SHC2	P29353 P98077 Q8WZ73 Q9UJF2	0:0:0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient Centrifugation	Mass spectrometryWestern blotting	1	27601599

Sequence, Structure & Domains8

Sequences

Length

1,139

Mass

128,558

Sequence

MQTPEVPAERSPRRRSISGTSTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRSTDDRSRGLPKLKESRSHESLLSPCSTVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFFSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDERESSLPNGRSVSLMDLQDTHAAQVEHASVMLDVPIRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGGLQPLSFQNPVYHLNNPIPAMPKASIDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKVDQGGLGARAKAPPSLPHSASLRSTGSMSVVSAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNLDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9UJF2-1; Sequence=Displayed; Name=2; IsoId=Q9UJF2-2; Sequence=VSP_045777, VSP_045778

Alternative Sequence

1..4; MQTP -> MELSPSSGGAAEALSWPEMFPALESDSPLPPEDLDAVVPVSGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKL (in isoform 2); 590..596; Missing (in isoform 2)

Domain & Motif Annotations

Compositional Bias

17..36; Polar residues; 45..56; Basic residues; 73..87; Basic and acidic residues; 751..760; Polar residues; 803..818; Polar residues; 833..855; Basic and acidic residues; 916..928; Low complexity

Domain (FT)

41..158; PH; 149..267; C2; 343..551; Ras-GAP

Region

1..87; Disordered; 684..704; Disordered; 751..782; Disordered; 803..869; Disordered; 910..953; Disordered; 1116..1139; Disordered

Clinical Relevance4

Antibody (2)

HPA018805 HPA020453

Interaction Protein (7)

ENSG00000108953ENSG00000113712ENSG00000128245ENSG00000134308ENSG00000166913ENSG00000170027ENSG00000171444

Interaction Count

Interaction Dataset (3)

biogrid_opencellintact_biogrid_opencellintact_biogrid_bioplex

Supporting Publications1

PMID	Title	Abstract
37324943	Heme-binding protein 1 delivered via pericyte-derived extracellular vesicles improves neurovascular regeneration in a mouse model of cavernous nerve injury.	No abstract available