Protein detail
ASAP1
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 (130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein) (ADP-ribosylation factor-directed GTPase-activating protein 1) (ARF GTPase-activating protein 1) (Development and differentiation-enhancing factor 1) (DEF-1) (Differentiation-enhancing factor 1) (PIP2-dependent ARF1 GAP)
Entry name ASAP1 | UniProt ID | EVMP confidence score 0.63 |
Supporting publications (n) 7 | Transmembrane count | Protein classification Predicted intracellular proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 (130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein) (ADP-ribosylation factor-directed GTPase-activating protein 1) (ARF GTPase-activating protein 1) (Development and differentiation-enhancing factor 1) (DEF-1) (Differentiation-enhancing factor 1) (PIP2-dependent ARF1 GAP)
Protein Class
Predicted intracellular proteins
Protein Function
Predicted intracellular proteins
Ensembl
Entrez Gene Symbol
Gene Synonym (5)
CENTB4DDEF1KIAA1249PAPZG14P
Gene Description
ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
Chromosome
8
Position
130052104-130443674
Supporting publications (n)
7
EVMP confidence score
0.63
Fluorescence & Localization6
Tissue Specificadipose tissueCell SpecificB-cellsSingle-Nuclei Brain SpecificfibroblastBlood Cell SpecificbasophilBlood Lineage Specificgranulocytes
Function & Pathway8
Protein Function
Predicted intracellular proteins
Cellular Component (6)
Molecular Function (7)
Biological Process (3)
Reactome (4)
Canonical Pathways (3)
- M56 Pid lpa4 pathway
- M8 Pid endothelin pathway
- M15 Pid lysophospholipid pathway
Mediation Categories (2)
Fusion and delivery mediationReceptor-signaling mediation
Relations & Evidence11
Enzyme-Mediated Modification (3)
3 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| ASAP1 | PTK2B | Q14289 | Y | 767 | phosphorylation | SIGNOR | SIGNOR:12771146 |
| ASAP1 | PTK2B | Q14289 | Y | 323 | phosphorylation | SIGNOR | SIGNOR:12771146 |
| ASAP1 | SRC | P12931 | Y | 767 | phosphorylation | REACH_ProtMapperSparser_ProtMapperProtMapper | ProtMapper:24957964 |
Ligand-Receptor Signaling (5)
5 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
Regulatory Interaction Network (1)
1 record.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| FAK2 | Q14289 | ASAP1 | Q9ULH1 | Yes | Yes | Yes | WangAdhesomeiPTMnetSIGNORProtMapperHPRDKEAphosphoELM_KEASIGNOR_ProtMapper | KEA:12771146ProtMapper:12771146HPRD:12771146Adhesome:12771146SIGNOR:12771146 |
Protein Complex Composition (1)
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationSize Exclusion Chromatography | Mass spectrometryMass spectrometry [LTQ-FT Ultra]Mass spectrometry [QTOF]R Sequencing | 1 | 38037300 |
Sequence, Structure & Domains15
Sequences
Length
1,129
Mass
125,498
Sequence
MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSTSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTVLHYCSMYSKPECLKLLLRSKPTVDIVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEIDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPTTEAPPLPPRNAGKGPTGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDGGPSSSSKTTNKFEGLSQQSSTSSAKTALGPRVLPKLPQKVALRKTDHLSLDKATIPPEIFQKSSQLAELPQKPPPGDLPPKPTELAPKPQIGDLPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQTGDVSPKAQQPSEVTLKSHPLDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=2; IsoId=Q9ULH1-1; Sequence=Displayed; Name=1; IsoId=Q9ULH1-2; Sequence=VSP_008365
Alternative Sequence
303; E -> ESRR (in isoform 1)
3D Structural Models
Turn
370..372
Helix
401..419; 1121..1123
Beta Strand
327..334; 336..338; 342..350; 353..356; 366..369; 373..377; 379..383; 385..389; 392..397; 1070..1076; 1081..1085; 1093..1098; 1101..1109; 1116..1120; 1124..1126
3D Structure
NMR spectroscopy (4); X-ray crystallography (1)
Domain & Motif Annotations
Compositional Bias
713..722; Acidic residues; 723..734; Basic and acidic residues; 735..747; Polar residues; 777..788; Low complexity; 809..822; Low complexity; 862..872; Polar residues; 876..889; Low complexity; 932..943; Pro residues; 956..966; Pro residues; 1001..1018; Polar residues
Repeat
600..632; ANK 1; 636..665; ANK 2
Zinc Finger
454..477; C4-type
Domain (CC)
The PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.
Domain (FT)
324..416; PH; 439..560; Arf-GAP; 1067..1129; SH3
Region
713..760; Disordered; 776..1062; Disordered
Clinical Relevance4
Supporting Publications7
| PMID | Title | Abstract |
|---|---|---|
| 36064647 | Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells. | No abstract available |
| 37322475 | Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration. | No abstract available |
| 38207106 | Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity. | No abstract available |
| 39207047 | The trajectory of vesicular proteomic signatures from HBV-HCC by chitosan-magnetic bead-based separation and DIA-proteomic analysis. | No abstract available |
| 40465195 | Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome. | No abstract available |
| 40840701 | Multiomics analysis to evaluate the enrichment of extracellular vesicles from human plasma. | No abstract available |
| 40985879 | TurboID-Mediated Profiling of Glioblastoma-Derived Extracellular Vesicle Cargo Proteins. | No abstract available |