Protein detail

PACN2

Protein kinase C and casein kinase substrate in neurons protein 2 (Syndapin-2) (Syndapin-II) (SdpII)

Entry name
PACN2
UniProt ID
EVMP confidence score
0.88
Supporting publications (n)
29
Transmembrane count
Protein classification
Predicted intracellular proteins
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information11
Protein Names
Protein kinase C and casein kinase substrate in neurons protein 2 (Syndapin-2) (Syndapin-II) (SdpII)
Protein Class
Predicted intracellular proteins
Protein Function
Predicted intracellular proteins
Entrez Gene Symbol
Gene Synonym
SDPII
Gene Description
Protein kinase C and casein kinase substrate in neurons 2
Chromosome
22
Position
42835412-43015149
Supporting publications (n)
29
EVMP confidence score
0.88
Fluorescence & Localization7
Tissue Specificbone marrowCell SpecificcDCSingle-Nuclei Brain Specificcentral nervous system macrophageBlood Cell SpecificneutrophilBlood Lineage SpecificgranulocytesSecretome LocationIntracellular and membraneSecretome FunctionReceptor
Function & Pathway6
Relations & Evidence15

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
PACSIN2PRKCAP17252S313phosphorylationPhosphoSite

Ligand-Receptor Signaling (9)

9 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
intracellularintracellularLOCATENoNoNoNoNo
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo
fbar_actin_dynamics_endocytosisintracellular_intercellular_relatedHGNCYesNoNoNoNo
intracellular_intercellular_relatedintracellular_intercellular_relatedOmniPathYesNoNoNoNo
plasma_membraneplasma_membraneUniProt_locationNoNoNoNoNo
plasma_membraneplasma_membraneOmniPathNoNoNoNoNo

Regulatory Interaction Network (2)

2 records.

Source Protein SymbolSource UniProt IDTarget Protein SymbolTarget UniProt IDIs DirectedIs StimulationIs InhibitionDatabaseReferences
KAPCAP17612PACN2Q9UNF0YesNoYesSIGNORSIGNOR:31801866
KPCAP17252PACN2Q9UNF0YesNoNoREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapperProtMapper:31801866PhosphoSite:31801866

Protein Complex Composition (2)

2 records.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
PACSIN2THG1LQ9NWX6Q9UNF00:0hu.MAPhu.MAP2
PACSIN2Q9UNF02PDBPDB:3hajPDB:3q0kPDB:3acoPDB:3abh

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationSize Exclusion ChromatographyMass spectrometryWestern blotting138716512
Sequence, Structure & Domains17

Sequences

Length
486
Mass
55,739
Sequence
MSVTYDDSVGVEVSSDSFWEVGNYKRTVKRIDDGHRLCSDLMNCLHERARIEKAYAQQLTEWARRWRQLVEKGPQYGTVEKAWMAFMSEAERVSELHLEVKASLMNDDFEKIKNWQKEAFHKQMMGGFKETKEAEDGFRKAQKPWAKKLKEVEAAKKAHHAACKEEKLAISREANSKADPSLNPEQLKKLQDKIEKCKQDVLKTKEKYEKSLKELDQGTPQYMENMEQVFEQCQQFEEKRLRFFREVLLEVQKHLDLSNVAGYKAIYHDLEQSIRAADAVEDLRWFRANHGPGMAMNWPQFEEWSADLNRTLSRREKKKATDGVTLTGINQTGDQSLPSKPSSTLNVPSNPAQSAQSQSSYNPFEDEDDTGSTVSEKDDTKAKNVSSYEKTQSYPTDWSDDESNNPFSSTDANGDSNPFDDDATSGTEVRVRALYDYEGQEHDELSFKAGDELTKMEDEDEQGWCKGRLDNGQVGLYPANYVEAIQ
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9UNF0-1; Sequence=Displayed; Name=2; IsoId=Q9UNF0-2; Sequence=VSP_004517
Alternative Sequence
344..384; Missing (in isoform 2)

3D Structural Models

Turn
21..24; 192..195
Helix
25..72; 77..106; 108..119; 129..169; 172..177; 185..188; 189..191; 197..255; 257..259; 263..275; 279..290
Beta Strand
126..128
3D Structure
X-ray crystallography (4)

Domain & Motif Annotations

Compositional Bias
327..347; Polar residues; 348..363; Low complexity; 383..396; Polar residues; 404..416; Polar residues
Motif
362..364; NPF1; 405..407; NPF2; 417..419; NPF3
Coiled Coil
25..274
Domain (CC)
The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation (PubMed:19549836). Autoinhibition of these functions is mediated by an interaction between the SH3 and F-BAR domains (PubMed:20188097, PubMed:23236520). The F-Bar domain also mediates the binding to the cell actin cytoskeleton through the interaction with CAV-1 (By similarity).; DOMAIN: (Microbial infection) The SH3 domain is required for the cell-to-cell spreading of HIV-1 virions.
Domain (FT)
11..282; F-BAR; 426..486; SH3
Region
1..362; (Microbial infection) Interaction with HCV NS5A; 314..426; Disordered
Protein Families
PACSIN family
Sequence Similarities
Belongs to the PACSIN family.
Clinical Relevance5
Interaction Protein (10)
ENSG00000039560ENSG00000082438ENSG00000100106ENSG00000100139ENSG00000106078ENSG00000106299ENSG00000117560ENSG00000124507ENSG00000133030ENSG00000165912
Interaction Count
10
Interaction Dataset (3)
biogrid_opencellintact_biogrid_opencellintact_biogrid
Supporting Publications29
PMIDTitleAbstract
26826536[Cardiovascular risk study in patients with renin-angiotensin system blockade by means of the proteone of circulating extracellular vesicles].No abstract available
27821849Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation.No abstract available
31382537An Insight into the Proteome of Uveal Melanoma-Derived Ectosomes Reveals the Presence of Potentially Useful Biomarkers.No abstract available
31805958Proteomic analysis of cerebrospinal fluid extracellular vesicles reveals synaptic injury, inflammation, and stress response markers in HIV patients with cognitive impairment.No abstract available
32230970Radiation Exposure of Peripheral Mononuclear Blood Cells Alters the Composition and Function of Secreted Extracellular Vesicles.No abstract available
32560723T2 and T17 cytokines alter the cargo and function of airway epithelium-derived extracellular vesicles.No abstract available
32854315Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study.Recent studies have highlighted the importance of Aβ and tau-containing extracellular vesicles (EVs) in AD.
32916986Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines.No abstract available
33304478The proteomic landscape of small urinary extracellular vesicles during kidney transplantation.No abstract available
33592500A Proteomic Approach to Understand the Clinical Significance of Acute Myeloid Leukemia-Derived Extracellular Vesicles Reflecting Essential Characteristics of Leukemia.No abstract available
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