Protein detail

PACN2

Protein kinase C and casein kinase substrate in neurons protein 2 (Syndapin-2) (Syndapin-II) (SdpII)

Entry name
PACN2
UniProt ID
EVMP confidence score
0.88
Supporting publications (n)
29
Transmembrane count
Protein classification
Predicted intracellular proteins
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information11
Protein Names
Protein kinase C and casein kinase substrate in neurons protein 2 (Syndapin-2) (Syndapin-II) (SdpII)
Protein Class
Predicted intracellular proteins
Protein Function
Predicted intracellular proteins
Entrez Gene Symbol
Gene Synonym
SDPII
Gene Description
Protein kinase C and casein kinase substrate in neurons 2
Chromosome
22
Position
42835412-43015149
Supporting publications (n)
29
EVMP confidence score
0.88
Fluorescence & Localization7
Tissue Specificbone marrowCell SpecificcDCSingle-Nuclei Brain Specificcentral nervous system macrophageBlood Cell SpecificneutrophilBlood Lineage SpecificgranulocytesSecretome LocationIntracellular and membraneSecretome FunctionReceptor
Function & Pathway6
Relations & Evidence15

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
PACSIN2PRKCAP17252S313phosphorylationPhosphoSite

Ligand-Receptor Signaling (9)

9 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
intracellularintracellularLOCATENoNoNoNoNo
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo
fbar_actin_dynamics_endocytosisintracellular_intercellular_relatedHGNCYesNoNoNoNo
intracellular_intercellular_relatedintracellular_intercellular_relatedOmniPathYesNoNoNoNo
plasma_membraneplasma_membraneUniProt_locationNoNoNoNoNo
plasma_membraneplasma_membraneOmniPathNoNoNoNoNo

Regulatory Interaction Network (2)

2 records.

Source Protein SymbolSource UniProt IDTarget Protein SymbolTarget UniProt IDIs DirectedIs StimulationIs InhibitionDatabaseReferences
KAPCAP17612PACN2Q9UNF0YesNoYesSIGNORSIGNOR:31801866
KPCAP17252PACN2Q9UNF0YesNoNoREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapperProtMapper:31801866PhosphoSite:31801866

Protein Complex Composition (2)

2 records.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
PACSIN2THG1LQ9NWX6Q9UNF00:0hu.MAPhu.MAP2
PACSIN2Q9UNF02PDBPDB:3hajPDB:3q0kPDB:3acoPDB:3abh

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationSize Exclusion ChromatographyMass spectrometryWestern blotting138716512
Sequence, Structure & Domains17

Sequences

Length
486
Mass
55,739
Sequence
MSVTYDDSVGVEVSSDSFWEVGNYKRTVKRIDDGHRLCSDLMNCLHERARIEKAYAQQLTEWARRWRQLVEKGPQYGTVEKAWMAFMSEAERVSELHLEVKASLMNDDFEKIKNWQKEAFHKQMMGGFKETKEAEDGFRKAQKPWAKKLKEVEAAKKAHHAACKEEKLAISREANSKADPSLNPEQLKKLQDKIEKCKQDVLKTKEKYEKSLKELDQGTPQYMENMEQVFEQCQQFEEKRLRFFREVLLEVQKHLDLSNVAGYKAIYHDLEQSIRAADAVEDLRWFRANHGPGMAMNWPQFEEWSADLNRTLSRREKKKATDGVTLTGINQTGDQSLPSKPSSTLNVPSNPAQSAQSQSSYNPFEDEDDTGSTVSEKDDTKAKNVSSYEKTQSYPTDWSDDESNNPFSSTDANGDSNPFDDDATSGTEVRVRALYDYEGQEHDELSFKAGDELTKMEDEDEQGWCKGRLDNGQVGLYPANYVEAIQ
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9UNF0-1; Sequence=Displayed; Name=2; IsoId=Q9UNF0-2; Sequence=VSP_004517
Alternative Sequence
344..384; Missing (in isoform 2)

3D Structural Models

Turn
21..24; 192..195
Helix
25..72; 77..106; 108..119; 129..169; 172..177; 185..188; 189..191; 197..255; 257..259; 263..275; 279..290
Beta Strand
126..128
3D Structure
X-ray crystallography (4)

Domain & Motif Annotations

Compositional Bias
327..347; Polar residues; 348..363; Low complexity; 383..396; Polar residues; 404..416; Polar residues
Motif
362..364; NPF1; 405..407; NPF2; 417..419; NPF3
Coiled Coil
25..274
Domain (CC)
The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation (PubMed:19549836). Autoinhibition of these functions is mediated by an interaction between the SH3 and F-BAR domains (PubMed:20188097, PubMed:23236520). The F-Bar domain also mediates the binding to the cell actin cytoskeleton through the interaction with CAV-1 (By similarity).; DOMAIN: (Microbial infection) The SH3 domain is required for the cell-to-cell spreading of HIV-1 virions.
Domain (FT)
11..282; F-BAR; 426..486; SH3
Region
1..362; (Microbial infection) Interaction with HCV NS5A; 314..426; Disordered
Protein Families
PACSIN family
Sequence Similarities
Belongs to the PACSIN family.
Clinical Relevance5
Interaction Protein (10)
ENSG00000039560ENSG00000082438ENSG00000100106ENSG00000100139ENSG00000106078ENSG00000106299ENSG00000117560ENSG00000124507ENSG00000133030ENSG00000165912
Interaction Count
10
Interaction Dataset (3)
biogrid_opencellintact_biogrid_opencellintact_biogrid
Supporting Publications29
PMIDTitleAbstract
38590132Proteomic Identification of Small Extracellular Vesicle Proteins LAMB1 and Histone H4 for Prostate Cancer Diagnosis and Risk Stratification.No abstract available
38716512Assessment of urine sample collection and processing variables for extracellular vesicle-based proteomics.No abstract available
38891077Altered Extracellular Vesicle-Derived Protein and microRNA Signatures in Bronchoalveolar Lavage Fluid from Patients with Chronic Obstructive Pulmonary Disease.No abstract available
39207047The trajectory of vesicular proteomic signatures from HBV-HCC by chitosan-magnetic bead-based separation and DIA-proteomic analysis.No abstract available
40189497Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.No abstract available
40465195Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome.No abstract available
40840701Multiomics analysis to evaluate the enrichment of extracellular vesicles from human plasma.No abstract available
40985879TurboID-Mediated Profiling of Glioblastoma-Derived Extracellular Vesicle Cargo Proteins.No abstract available
41068253Identification of plasma extracellular vesicle protein biomarkers in diabetic retinopathy progression.No abstract available
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