Protein detail

UBP20

Ubiquitin carboxyl-terminal hydrolase 20 (EC 3.4.19.12) (Deubiquitinating enzyme 20) (Ubiquitin thioesterase 20) (Ubiquitin-specific-processing protease 20) (VHL-interacting deubiquitinating enzyme 2) (hVDU2)

Entry name UBP20	UniProt ID Q9Y2K6	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification EnzymesMetabolic proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Protein Class (3)

EnzymesMetabolic proteinsPredicted intracellular proteins

Protein Function (4)

Peptidases:Cysteine-type peptidases
Enzymes
Predicted intracellular proteins
ENZYME proteins:Hydrolases

Ensembl

ENSG00000136878

Entrez Gene Symbol

USP20

Gene Synonym

KIAA1003

Gene Description

Ubiquitin specific peptidase 20

Chromosome

Position

129834698-129881828

Supporting publications (n)

EVMP confidence score

0.38

Function & Pathway6

Protein Function (4)

Peptidases:Cysteine-type peptidases
Enzymes
Predicted intracellular proteins
ENZYME proteins:Hydrolases

Cellular Component (5)

Molecular Function (5)

Biological Process (3)

Reactome (3)

Mediation Categories (3)

Clinical-translation mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence19

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
USP20	PRKACA	P17612	S	333	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper

Ligand-Receptor Signaling (7)

7 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
gpcr	receptor	DGIdb	No	Yes	No	No	No
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (5)

5 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
	COMPLEX:P40337_P62877_Q13617_Q15369_Q15370	UBP20	Q9Y2K6	Yes	No	Yes	SIGNOR	SIGNOR:12056827
ATR	Q13535	UBP20	Q9Y2K6	Yes	Yes	Yes	SIGNOR	SIGNOR:25355518
HERC2	O95714	UBP20	Q9Y2K6	Yes	No	Yes	HINTSIGNOR	HINT:19615732 SIGNOR:25355518 HINT:29426014 HINT:25355518 SIGNOR:25326330
KAPCA	P17612	UBP20	Q9Y2K6	Yes	No	Yes	iPTMnetSIGNORProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:25666616 SIGNOR:25666616
UBP20	Q9Y2K6	CLSPN	Q9HAW4	Yes	Yes	No	HINTSIGNOR	HINT:25355518 HINT:25326330 SIGNOR:25355518

Protein Complex Composition (5)

5 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
HT_SC_Cluster390	DHX30 DHX34 USP17L10 USP17L11 USP17L12 USP17L13 USP17L4 USP17L7 USP17L8 USP20 USP33 USP36 USP42	A6NCW7 C9JJH3 C9JLJ4 C9JPN9 C9JVI0 P0C7H9 P0C7I0 Q14147 Q7L2E3 Q8TEY7 Q9H9J4 Q9P275 Q9Y2K6	1:1:1:1:1:1:1:1:1:1:1:1:1	Compleat	Compleat:HC1702
	ATAD3B IPO5 KPNB1 NUP153 NUP98 NUTF2 RAN RANBP2 RCC1 RGPD6 TNPO1 UBC USP20	O00410 P0CG48 P18754 P49790 P49792 P52948 P61970 P62826 Q14974 Q5T9A4 Q92973 Q99666 Q9Y2K6	1:1:1:1:1:1:1:1:1:1:1:2:1	NetworkBlastCompleat	Compleat:HC7963
	APBA1 APBA2 CBX7 IQSEC2 SCML2 USP20	O95931 Q02410 Q5JU85 Q99767 Q9UQR0 Q9Y2K6	0:0:0:0:0:0	hu.MAP2
	CBX7 USP20	O95931 Q9Y2K6	0:0	hu.MAP2
	APBA1 APBA2 IQSEC2 USP20	Q02410 Q5JU85 Q99767 Q9Y2K6	0:0:0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Western blotting	2	40098346 37922300

Sequence, Structure & Domains14

Sequences

Length

914

Mass

102,003

Sequence

MGDSRDLCPHLDSIGEVTKEDLLLKSKGTCQSCGVTGPNLWACLQVACPYVGCGESFADHSTIHAQAKKHNLTVNLTTFRLWCYACEKEVFLEQRLAAPLLGSSSKFSEQDSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLVSEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPVVATVALTEARDSDSSDTDEKREGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSQAETELLIPDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMAALDDQPAEAQPPSPRSSSPCRTPEPDNDAHLRSSSRPCSPVHHHEGHAKLSSSPPRASPVRMAPSYVLKKAQVLSAGSRRRKEQRYRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYAAQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLRPFLAKECTSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEGYVLFYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGPITNQTFLCSHGGIPPHKYHYIDDLVVILPQNVWEHLYNRFGGGPAVNHLYVCSICQVEIEALAKRRRIEIDTFIKLNKAFQAEESPGVIYCISMQWFREWEAFVKGKDNEPPGPIDNSRIAQVKGSGHVQLKQGADYGQISEETWTYLNSLYGGGPEIAIRQSVAQPLGPENLHGEQKIEAETRAV

3D Structural Models

Turn

10..13; 31..33; 55..58; 76..78; 84..87

Helix

19..25; 60..67

Beta Strand

4..7; 41..43; 72..74; 81..83; 88..90

3D Structure

NMR spectroscopy (1)

Domain & Motif Annotations

Compositional Bias

259..279; Basic and acidic residues; 316..332; Basic and acidic residues

Zinc Finger

6..111; UBP-type

Domain (CC)

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue (By similarity).

Domain (FT)

145..685; USP; 687..780; DUSP 1; 789..892; DUSP 2

Region

257..347; Disordered; 360..415; Disordered

Protein Families (2)

Peptidase C19 family
USP20/USP33 subfamily

Sequence Similarities

Belongs to the peptidase C19 family. USP20/USP33 subfamily.

Clinical Relevance6

Related Diseases

Solid tumour/cancer

Biomarker

Preclinical

Antibody (2)

HPA006287 HPA007008

Interaction Protein (3)

ENSG00000100307ENSG00000101558ENSG00000124164

Interaction Count

Interaction Dataset (2)

biogrid_bioplexintact_biogrid

Supporting Publications1

PMID	Title	Abstract
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available