Protein detail

UB2D4

Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)

Entry name UB2D4	UniProt ID Q9Y2X8	EVMP confidence score 0.38
Supporting publications (n) 5	Transmembrane count	Protein classification EnzymesMetabolic proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)

Protein Class (3)

EnzymesMetabolic proteinsPredicted intracellular proteins

Protein Function (3)

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins

Ensembl

ENSG00000078967

Entrez Gene Symbol

UBE2D4

Gene Synonym

HBUCE1

Gene Description

Ubiquitin conjugating enzyme E2 D4 (putative)

Chromosome

Position

43926436-43956136

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization3

Tissue Specificblood vesselCell SpecificBrain inhibitory neuronsSingle-Nuclei Brain Specificpericyte

Function & Pathway7

Protein Function (3)

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins

Cellular Component

GO:0005634 nucleus

Molecular Function (4)

Biological Process (3)

KEGG (4)

Reactome (3)

Mediation Categories

Immune mediation

Relations & Evidence18

Ligand-Receptor Signaling (3)

3 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (8)

8 records.

Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
COMPLEX:A0AVT1_P0CG47	UB2D4	Q9Y2X8	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:A0AVT1_P62979	UB2D4	Q9Y2X8	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:A0AVT1_P0CG48	UB2D4	Q9Y2X8	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:A0AVT1_P62987	UB2D4	Q9Y2X8	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P22314_P62979	UB2D4	Q9Y2X8	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P22314_P62987	UB2D4	Q9Y2X8	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P0CG47_P22314	UB2D4	Q9Y2X8	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P0CG48_P22314	UB2D4	Q9Y2X8	Yes	Yes	No	SIGNOR	SIGNOR:34199813

Protein Complex Composition (6)

6 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
Ub:E2	UBB UBE2D4	P0CG47 Q9Y2X8	0:0	SIGNOR	SIGNOR:SIGNOR-C497
Ub:E2	UBC UBE2D4	P0CG48 Q9Y2X8	0:0	SIGNOR	SIGNOR:SIGNOR-C497
Ub:E2	RPS27A UBE2D4	P62979 Q9Y2X8	0:0	SIGNOR	SIGNOR:SIGNOR-C497
Ub:E2	UBA52 UBE2D4	P62987 Q9Y2X8	0:0	SIGNOR	SIGNOR:SIGNOR-C497
	ALDH1A2 IPO5 KPNB1 NUP153 NUP214 NUP98 NUTF2 RAN RANBP2 TNPO1 UBC UBE2D4 XPO1 XPOT	O00410 O14980 O43592 O94788 P0CG48 P35658 P49790 P49792 P52948 P61970 P62826 Q14974 Q92973 Q9Y2X8	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7166
	BTRC CUL1 FBXL12 FBXO33 FBXO42 FBXO5 RBX1 SKP1 UBE2D4 USP47	P62877 P63208 Q13616 Q6P3S6 Q7Z6M2 Q96K76 Q9NXK8 Q9UKT4 Q9Y297 Q9Y2X8	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8648

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
	Mass spectrometry	0

Sequence, Structure & Domains6

Sequences

Length

147

Mass

16,649

Sequence

MALKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWTQKYAM

Domain & Motif Annotations

Domain (FT)

1..147; UBC core

Protein Families

Ubiquitin-conjugating enzyme family

Sequence Similarities

Belongs to the ubiquitin-conjugating enzyme family.

Clinical Relevance4

Antibody

HPA003920

Interaction Protein (25)

ENSG00000013561ENSG00000022840ENSG00000070423ENSG00000080561ENSG00000091073ENSG00000101871ENSG00000121481ENSG00000123091ENSG00000138942ENSG00000146755ENSG00000157450ENSG00000158717ENSG00000159461ENSG00000163481ENSG00000165886ENSG00000167770ENSG00000168411ENSG00000168894ENSG00000171206ENSG00000178498ENSG00000186187ENSG00000204227ENSG00000204308ENSG00000204599ENSG00000265491

Interaction Count

Interaction Dataset (2)

intact_biogridintact_biogrid_bioplex

Supporting Publications5

PMID	Title	Abstract
26801919	Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis.	No abstract available
30071318	Changes in the urinary extracellular vesicle proteome are associated with nephronophthisis-related ciliopathies.	No abstract available
31685984	Tumour exosomal CEMIP protein promotes cancer cell colonization in brain metastasis.	Proteomic analysis identified cell migration-inducing and hyaluronan-binding protein (CEMIP) as elevated in exosomes from brain metastatic but not lung or bone metastatic cells.
38136601	Identification of Central Nervous System Oncologic Disease Biomarkers in EVs from Cerebrospinal Fluid (CSF) of Pediatric Patients: A Pilot Neuro-Proteomic Study.	No abstract available
38731868	The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer.	No abstract available