Protein detail

CLIC4

Chloride intracellular channel protein 4 (Glutaredoxin-like oxidoreductase CLIC4) (EC 1.8.-.-) (Intracellular chloride ion channel protein p64H1)

Entry name
CLIC4
UniProt ID
Q9Y696
EVMP confidence score
0.88
Supporting publications (n)
41
Transmembrane count
1
Protein classification
Predicted intracellular proteinsTransporters
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information13
Protein Names
Chloride intracellular channel protein 4 (Glutaredoxin-like oxidoreductase CLIC4) (EC 1.8.-.-) (Intracellular chloride ion channel protein p64H1)
Protein Class (2)
Predicted intracellular proteinsTransporters
Protein Function (2)
  • Transporters:Transporter channels and pores
  • Predicted intracellular proteins
Transmembrane
37..57; Helical; Note=After insertion into the membrane
Transmembrane Count
1
Ensembl
ENSG00000169504
Entrez Gene Symbol
CLIC4
Gene Synonym (5)
CLIC4LDKFZP566G223H1huH1P64H1
Gene Description
Chloride intracellular channel 4
Chromosome
1
Position
24745382-24844321
Supporting publications (n)
41
EVMP confidence score
0.88
Fluorescence & Localization4
CLIC4 fluorescence
Cell SpecificAdrenal medulla cellsBlood Cell SpecificeosinophilBlood Lineage Specificgranulocytes
Function & Pathway6
Protein Function (2)
  • Transporters:Transporter channels and pores
  • Predicted intracellular proteins
Cellular Component (19)
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Molecular Function (3)
Biological Process (3)
Canonical Pathways (3)
  • M5883 Naba secreted factors
  • M5885 Naba matrisome associated
  • M5889 Naba matrisome
Mediation Categories (2)
Clinical-translation mediationFusion and delivery mediation
Relations & Evidence21

Ligand-Receptor Signaling (11)

11 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
plasma_membraneplasma_membraneOmniPathNoNoNoNoNo
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Regulatory Interaction Network (1)

1 record.

Source Protein SymbolSource UniProt IDTarget Protein SymbolTarget UniProt IDIs DirectedIs StimulationIs InhibitionDatabaseReferences
CDK5Q00535CLIC4Q9Y696YesYesNoSIGNORSparser_ProtMapperProtMapperSIGNOR:30237421ProtMapper:30237421

Protein Complex Composition (8)

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyImmunoaffinity CaptureMass spectrometry6381133683732247541068253335925004130796838607062
Sequence, Structure & Domains13

Sequences

Length
253
Mass
28,772
Sequence
MALSMPLNGLKEEDKEPLIELFVKAGSDGESIGNCPFSQRLFMILWLKGVVFSVTTVDLKRKPADLQNLAPGTHPPFITFNSEVKTDVNKIEEFLEEVLCPPKYLKLSPKHPESNTAGMDIFAKFSAYIKNSRPEANEALERGLLKTLQKLDEYLNSPLPDEIDENSMEDIKFSTRKFLDGNEMTLADCNLLPKLHIVKVVAKKYRNFDIPKEMTGIWRYLTNAYSRDEFTNTCPSDKEVEIAYSDVAKRLTK

3D Structural Models

Turn
101..103; 116..120; 244..247
Helix
36..48; 64..69; 88..98; 112..115; 121..130; 134..136; 137..156; 186..206; 215..225; 228..231; 237..243
Beta Strand
19..25; 29..32; 53..57; 77..80; 83..85; 181..183
3D Structure
X-ray crystallography (3)

Domain & Motif Annotations

Motif
35..38; G-site
Domain (CC)
The active G-site contains a monothiol Cys-X-X-Ser motif which mediates glutathione-dependent redox catalysis.; DOMAIN: Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as a chloride channel. The redox status of the active cysteine in Cys-X-X-Cys/Ser motif likely determines the capacity to adopt a soluble or membrane-inserted state. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
Domain (FT)
81..244; GST C-terminal
Region
2..101; Required for insertion into the membrane
Protein Families
Chloride channel CLIC family
Sequence Similarities
Belongs to the chloride channel CLIC family.
Clinical Relevance5
Drug Targets
Literature-reported target
Antibody (2)
HPA008019HPA060804
Interaction Protein
ENSG00000159212
Interaction Count
1
Interaction Dataset
intact_biogrid
Supporting Publications41
PMIDTitleAbstract
34108659Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker.Syntenin-1 is also present in exosomes across different species and biofluids, highlighting its potential use as a putative universal biomarker of exosomes. We identified syntenin-1 as a consistently abundant protein in exosomes from different cellular origins.
34202855Proteomic Profiling of Ectosomes Derived from Paired Urothelial Bladder Cancer and Normal Cells Reveals the Presence of Biologically-Relevant Molecules.No abstract available
34341426Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals.No abstract available
37309723Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells.No abstract available
37686366Identification of a Non-Invasive Urinary Exosomal Biomarker for Diabetic Nephropathy Using Data-Independent Acquisition Proteomics.No abstract available
37862381Surfaceome analysis of extracellular vesicles from senescent cells uncovers uptake repressor DPP4.No abstract available
37926697Analysis of small EV proteomes reveals unique functional protein networks regulated by VAP-A.No abstract available
38164498Enrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification.No abstract available
38225453Deep proteomic analysis of obstetric antiphospholipid syndrome by DIA-MS of extracellular vesicle enriched fractions.No abstract available
38490958Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer.No abstract available
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