Protein detail
DDX3X
ATP-dependent RNA helicase DDX3X (EC 3.6.4.13) (CAP-Rf) (DEAD box protein 3, X-chromosomal) (DEAD box, X isoform) (DBX) (Helicase-like protein 2) (HLP2)
Entry name DDX3X | UniProt ID | EVMP score 0.38 |
Frequency 4 | Transmembrane count | Protein classification Cancer-related genesDisease related genesEnzymesEssential proteinsHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters |
EVMP score: annotation confidence score.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information
Protein Names
ATP-dependent RNA helicase DDX3X (EC 3.6.4.13) (CAP-Rf) (DEAD box protein 3, X-chromosomal) (DEAD box, X isoform) (DBX) (Helicase-like protein 2) (HLP2)
Protein Class
Cancer-related genesDisease related genesEnzymesEssential proteinsHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters
Protein Function
- Human disease related genes:Other diseases:Mental and behavioural disorders
- Predicted intracellular proteins
- Cancer-related genes:Mutated cancer genes
- Potential drug targets
- Enzymes
- Transporters:Transporter channels and pores
- ENZYME proteins:Hydrolases
- Disease related genes
Ensembl
Entrez Gene Symbol
Gene Synonym
CAP-RfDBXDDX14DDX3HLP2
Gene Description
DEAD-box helicase 3 X-linked
Chromosome
X
Position
41333348-41364472
Frequency
4
EVMP Score
0.38
Fluorescence & Localization
Cell SpecificEarly spermatids
Function & Pathway
Protein Function
- Human disease related genes:Other diseases:Mental and behavioural disorders
- Predicted intracellular proteins
- Cancer-related genes:Mutated cancer genes
- Potential drug targets
- Enzymes
- Transporters:Transporter channels and pores
- ENZYME proteins:Hydrolases
- Disease related genes
Cellular Component
- GO:0005576 extracellular region
- GO:0005634 nucleus
- GO:0005654 nucleoplasm
- GO:0005737 cytoplasm
- GO:0005813 centrosome
- GO:0005829 cytosol
- GO:0005852 eukaryotic translation initiation factor 3 complex
- GO:0005886 plasma membrane
- GO:0010494 cytoplasmic stress granule
- GO:0022627 cytosolic small ribosomal subunit
- GO:0030027 lamellipodium
- GO:0031252 cell leading edge
- GO:0034774 secretory granule lumen
- GO:0043186 P granule
- GO:0070062 extracellular exosome
- GO:0072559 NLRP3 inflammasome complex
- GO:1904813 ficolin-1-rich granule lumen
Molecular Function
- GO:0003677 DNA binding
- GO:0003678 DNA helicase activity
- GO:0003723 RNA binding
- GO:0003724 RNA helicase activity
- GO:0003729 mRNA binding
- GO:0003924 GTPase activity
- GO:0005515 protein binding
- GO:0005524 ATP binding
- GO:0008134 transcription factor binding
- GO:0008143 poly(A) binding
- GO:0008190 eukaryotic initiation factor 4E binding
- GO:0016887 ATP hydrolysis activity
- GO:0017111 ribonucleoside triphosphate phosphatase activity
- GO:0031369 translation initiation factor binding
- GO:0033592 RNA strand annealing activity
- GO:0035591 signaling adaptor activity
- GO:0035613 RNA stem-loop binding
- GO:0043015 gamma-tubulin binding
- GO:0043024 ribosomal small subunit binding
- GO:0043273 CTPase activity
- GO:0043539 protein serine/threonine kinase activator activity
- GO:0045296 cadherin binding
- GO:0048027 mRNA 5'-UTR binding
Biological Process
KEGG
Reactome
- R-hsa-9920588 dengue virus activates modulates innate and adaptive immune responses
- R-hsa-9918481 dengue virus host interactions
- R-hsa-9839923 dengue virus infection
- R-hsa-5663205 infectious disease
- R-hsa-168249 innate immune system
- R-hsa-6798695 neutrophil degranulation
- R-hsa-9824446 viral infection pathways
Canonical Pathways
M177 Pid epha fwdpathway
Mediation Categories
Clinical-translation mediationImmune mediationReceptor-signaling mediation
Relations & Evidence
Enzyme-Mediated Modification
8 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| DDX3X | CDK1 | P06493 | T | 323 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:16280325phosphoELM:16280325SIGNOR:16280325KEA:16280325 |
| DDX3X | CDK1 | P06493 | T | 204 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | KEA:16280325ProtMapper:16280325phosphoELM:16280325SIGNOR:16280325ProtMapper:17379183 |
| DDX3X | CCNB1 | P14635 | T | 323 | phosphorylation | SIGNOR | SIGNOR:16280325 |
| DDX3X | CCNB1 | P14635 | T | 204 | phosphorylation | SIGNOR | SIGNOR:16280325 |
| DDX3X | IKBKE | Q14164 | S | 102 | phosphorylation | Sparser_ProtMapperProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:28402257ProtMapper:25538732 |
| DDX3X | CDK2 | P24941 | T | 204 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper | |
| DDX3X | AURKB | Q96GD4 | S | 594 | phosphorylation | PhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper | |
| DDX3X | IGF1R | P08069 | Y | 104 | phosphorylation | KEA | KEA:17570479 |
Ligand-Receptor Signaling
10 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| ecm | ecm | MatrixDB | Yes | No | No | No | No |
| ecm | ecm | OmniPath | Yes | No | No | No | No |
| extracellular | extracellular | OmniPath | No | No | No | No | No |
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| plasma_membrane | plasma_membrane | UniProt_location | No | No | No | No | No |
| plasma_membrane | plasma_membrane | OmniPath | No | No | No | No | No |
Regulatory Interaction Network
7 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| TBK1 | Q9UHD2 | DDX3X | O00571 | Yes | Yes | No | WangSPIKE_LCSIGNOR | SIGNOR:18583960SPIKE_LC:18583960 |
| DDX3X | O00571 | SP1 | P08047 | Yes | Yes | No | InnateDBSIGNOR | InnateDB:16818630SIGNOR:33627125 |
| DDX3X | O00571 | IF4E | P06730 | Yes | No | Yes | SIGNORELMHINTIntActInnateDBLMPID | HINT:21883093HINT:19262567IntAct:18596238IntAct:22872150HINT:17667941IntAct:21883093InnateDB:17667941LMPID:17667941ELM:17667941SIGNOR:17667941HINT:18596238 |
| DDX3X | O00571 | PABP1 | P11940 | Yes | Yes | No | HINTSIGNORIntAct | HINT:21883093IntAct:18596238IntAct:22872150HINT:22872150IntAct:21883093SIGNOR:21883093HINT:18596238 |
| FUS | P35637 | DDX3X | O00571 | Yes | No | Yes | SIGNOR | SIGNOR:27460707 |
| CDK1 | P06493 | DDX3X | O00571 | Yes | No | Yes | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperRLIMS-P_ProtMapperPhosphoSite_KEAKEAphosphoELM_KEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:16280325KEA:16280325ProtMapper:16280325phosphoELM:16280325PhosphoSite:17379183SIGNOR:16280325ProtMapper:17379183 |
| COMPLEX:P06493_P14635 | DDX3X | O00571 | Yes | No | Yes | SIGNOR | SIGNOR:16280325 |
Protein Complex Composition
43 records.
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Sequence, Structure & Domains
Sequences
Length
662
Mass
73,243
Sequence
MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O00571-1; Sequence=Displayed; Name=2; IsoId=O00571-2; Sequence=VSP_042830
Alternative Sequence
35..51; KGRYIPPHLRNREATKG -> S (in isoform 2)
3D Structural Models
Turn
158..161; 355..357; 411..414; 499..501; 502..504
Helix
144..151; 162..165; 182..184; 189..198; 205..215; 230..245; 249..256; 276..290; 306..313; 324..332; 349..354; 358..365; 387..396; 424..426; 427..437; 451..463; 477..480; 482..488; 522..529; 547..552; 553..562; 569..575; 578..580
Beta Strand
136..138; 168..172; 220..223; 261..263; 268..272; 297..300; 302..304; 318..322; 343..348; 367..369; 376..383; 401..405; 415..421; 444..449; 468..471; 473..475; 491..498; 509..517; 535..537; 539..545
3D Structure
X-ray crystallography (16)
Domain & Motif Annotations
Compositional Bias
21..34; Polar residues; 44..68; Basic and acidic residues; 70..89; Low complexity; 94..130; Basic and acidic residues; 604..622; Low complexity; 623..634; Gly residues
Motif
12..21; Nuclear export signal; 180..208; Q motif; 347..350; DEAD box
Domain (CC)
The C-terminus (residues 536-662) is dispensable for DDX3X trafficking.
Domain (FT)
211..403; Helicase ATP-binding; 414..575; Helicase C-terminal
Region
2..139; Required for TBK1 and IKBKE-dependent IFNB1 activation; 19..144; Disordered; 38..44; Interaction with EIF4E; 81..90; Interaction with VACV protein K7; 88..123; Involved in binding to RNA G-quadruplex; 100..662; Interaction with GSK3B; 100..110; Interaction with IKBKE; 139..172; Interaction with CHUK; 250..259; Involved in stimulation of ATPase activity by DNA and RNA, nucleic acid binding and unwinding and HIV-1 replication; 409..662; Interaction with HCV core protein; 536..661; Interaction with NXF1; 601..634; Disordered
Protein Families
- DEAD box helicase family
- DDX3/DED1 subfamily
Sequence Similarities
Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.