Protein detail

DDX3X

ATP-dependent RNA helicase DDX3X (EC 3.6.4.13) (CAP-Rf) (DEAD box protein 3, X-chromosomal) (DEAD box, X isoform) (DBX) (Helicase-like protein 2) (HLP2)

Entry name DDX3X	UniProt ID O00571	EVMP score 0.38
Frequency 4	Transmembrane count	Protein classification Cancer-related genesDisease related genesEnzymesEssential proteinsHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

ATP-dependent RNA helicase DDX3X (EC 3.6.4.13) (CAP-Rf) (DEAD box protein 3, X-chromosomal) (DEAD box, X isoform) (DBX) (Helicase-like protein 2) (HLP2)

Protein Class

Cancer-related genesDisease related genesEnzymesEssential proteinsHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters

Protein Function

Human disease related genes:Other diseases:Mental and behavioural disorders
Predicted intracellular proteins
Cancer-related genes:Mutated cancer genes
Potential drug targets
Enzymes
Transporters:Transporter channels and pores
ENZYME proteins:Hydrolases
Disease related genes

Ensembl

ENSG00000215301

Entrez Gene Symbol

DDX3X

Gene Synonym

CAP-RfDBXDDX14DDX3HLP2

Gene Description

DEAD-box helicase 3 X-linked

Chromosome

Position

41333348-41364472

Frequency

EVMP Score

0.38

Fluorescence & Localization

Cell SpecificEarly spermatids

Function & Pathway

Protein Function

Human disease related genes:Other diseases:Mental and behavioural disorders
Predicted intracellular proteins
Cancer-related genes:Mutated cancer genes
Potential drug targets
Enzymes
Transporters:Transporter channels and pores
ENZYME proteins:Hydrolases
Disease related genes

Cellular Component

Biological Process

KEGG

Reactome

Canonical Pathways

M177 Pid epha fwdpathway

Mediation Categories

Clinical-translation mediationImmune mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

8 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
DDX3X	CDK1	P06493	T	323	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:16280325 phosphoELM:16280325 SIGNOR:16280325 KEA:16280325
DDX3X	CDK1	P06493	T	204	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:16280325 ProtMapper:16280325 phosphoELM:16280325 SIGNOR:16280325 ProtMapper:17379183
DDX3X	CCNB1	P14635	T	323	phosphorylation	SIGNOR	SIGNOR:16280325
DDX3X	CCNB1	P14635	T	204	phosphorylation	SIGNOR	SIGNOR:16280325
DDX3X	IKBKE	Q14164	S	102	phosphorylation	Sparser_ProtMapperProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:28402257 ProtMapper:25538732
DDX3X	CDK2	P24941	T	204	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
DDX3X	AURKB	Q96GD4	S	594	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
DDX3X	IGF1R	P08069	Y	104	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling

10 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	MatrixDB	Yes	No	No	No	No
ecm	ecm	OmniPath	Yes	No	No	No	No
extracellular	extracellular	OmniPath	No	No	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Regulatory Interaction Network

7 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
TBK1	Q9UHD2	DDX3X	O00571	Yes	Yes	No	WangSPIKE_LCSIGNOR	SIGNOR:18583960 SPIKE_LC:18583960
DDX3X	O00571	SP1	P08047	Yes	Yes	No	InnateDBSIGNOR	InnateDB:16818630 SIGNOR:33627125
DDX3X	O00571	IF4E	P06730	Yes	No	Yes	SIGNORELMHINTIntActInnateDBLMPID	HINT:21883093 HINT:19262567 IntAct:18596238 IntAct:22872150 HINT:17667941 IntAct:21883093 InnateDB:17667941 LMPID:17667941 ELM:17667941 SIGNOR:17667941 HINT:18596238
DDX3X	O00571	PABP1	P11940	Yes	Yes	No	HINTSIGNORIntAct	HINT:21883093 IntAct:18596238 IntAct:22872150 HINT:22872150 IntAct:21883093 SIGNOR:21883093 HINT:18596238
FUS	P35637	DDX3X	O00571	Yes	No	Yes	SIGNOR	SIGNOR:27460707
CDK1	P06493	DDX3X	O00571	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperRLIMS-P_ProtMapperPhosphoSite_KEAKEAphosphoELM_KEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:16280325 KEA:16280325 ProtMapper:16280325 phosphoELM:16280325 PhosphoSite:17379183 SIGNOR:16280325 ProtMapper:17379183
	COMPLEX:P06493_P14635	DDX3X	O00571	Yes	No	Yes	SIGNOR	SIGNOR:16280325

Protein Complex Composition

43 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
DDX3X EIF4E EIF4G1 EIF4G3 HNRNPC KHDRBS3 NCBP1 NCBP2 PABPC1 PUF60 RBM39 SF3A2 SRSF9 TRNAU1AP U2AF2	O00571 O43432 O75525 P06730 P07910 P11940 P26368 P52298 Q04637 Q09161 Q13242 Q14498 Q15428 Q9NX07 Q9UHX1	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8143
CNBP DDX3X EIF4E EIF4G2 EIF4G3 HNRNPC KHDRBS3 NCBP1 NCBP2 PUF60 RBM42 RNPS1 SF3A2 SRSF6 U2AF2	O00571 O43432 O75525 P06730 P07910 P26368 P52298 P62633 P78344 Q09161 Q13247 Q15287 Q15428 Q9BTD8 Q9UHX1	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6961
DDX3X EIF4E EIF4G1 EIF4G3 KHDRBS3 NCBP1 NCBP2 PABPC1 POLA2 PRPF38A RBM23 SRSF9 TRNAU1AP	O00571 O43432 O75525 P06730 P11940 P52298 Q04637 Q09161 Q13242 Q14181 Q86U06 Q8NAV1 Q9NX07	1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7058
CELF2 CELF3 DDX39B DDX3X EIF4E EIF4G3 EWSR1 NCBP1 POLDIP3 SRSF2 THOC2 TRNAU1AP UBC	O00571 O43432 O95319 P06730 P0CG48 Q01130 Q01844 Q09161 Q13838 Q5SZQ8 Q8NI27 Q9BY77 Q9NX07	1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6534
CELF2 DDX3X EIF4G3 EWSR1 NCBP1 NCBP2 PABPC1 PCBP1 POLDIP3 PUF60 SF1 SF3A2 TRNAU1AP U2AF2	O00571 O43432 O95319 P11940 P26368 P52298 Q01844 Q09161 Q15365 Q15428 Q15637 Q9BY77 Q9NX07 Q9UHX1	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC3712
CDK18 DDX3X EIF4E EIF4G2 EIF4G3 EWSR1 HNRNPC KHDRBS2 LMO3 NCBP1 PUF60 RALYL RNPS1 U2AF2	O00571 O43432 P06730 P07910 P26368 P78344 Q01844 Q07002 Q09161 Q15287 Q5VWX1 Q86SE5 Q8TAP4 Q9UHX1	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4151
DDX3X DHX40 EIF4E EIF4G1 EIF4G3 G3BP1 NCBP1 NCBP2 PABPC1 POLDIP3 SNRPA1 TRNAU1AP UBC USP10 YTHDC2	O00571 O43432 P06730 P09661 P0CG48 P11940 P52298 Q04637 Q09161 Q13283 Q14694 Q8IX18 Q9BY77 Q9H6S0 Q9NX07	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5988
ATXN2L DDX3X EIF4E EIF4G1 EIF4G3 G3BP2 NCBP1 NCBP2 PABPC1 SF1 TRNAU1AP U2AF2 UBC USP10	O00571 O43432 P06730 P0CG48 P11940 P26368 P52298 Q04637 Q09161 Q14694 Q15637 Q8WWM7 Q9NX07 Q9UN86	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4591
DDX3X DHX40 EIF3B EIF4A1 EIF4A2 EIF4E EIF4G1 EIF4G2 EIF4G3 G3BP1 NCBP1 NCBP2 PABPC1 UBC USP10	O00571 O43432 P06730 P0CG48 P11940 P52298 P55884 P60842 P78344 Q04637 Q09161 Q13283 Q14240 Q14694 Q8IX18	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6084
CELF1 DDX3X EIF3B EIF4A1 EIF4A2 EIF4E EIF4G1 EIF4G3 G3BP1 GAR1 NCBP1 NCBP2 PABPC1 UBC USP10	O00571 O43432 P06730 P0CG48 P11940 P52298 P55884 P60842 Q04637 Q09161 Q13283 Q14240 Q14694 Q92879 Q9NY12	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5119

Page 2 of 5Previous Next

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometryAntibody array	6	38113368 40189497 33592500 38014595 36146834 27821849

Sequence, Structure & Domains

Sequences

Length

662

Mass

73,243

Sequence

MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O00571-1; Sequence=Displayed; Name=2; IsoId=O00571-2; Sequence=VSP_042830

Alternative Sequence

35..51; KGRYIPPHLRNREATKG -> S (in isoform 2)

3D Structural Models

Turn

158..161; 355..357; 411..414; 499..501; 502..504

Helix

144..151; 162..165; 182..184; 189..198; 205..215; 230..245; 249..256; 276..290; 306..313; 324..332; 349..354; 358..365; 387..396; 424..426; 427..437; 451..463; 477..480; 482..488; 522..529; 547..552; 553..562; 569..575; 578..580

Beta Strand

136..138; 168..172; 220..223; 261..263; 268..272; 297..300; 302..304; 318..322; 343..348; 367..369; 376..383; 401..405; 415..421; 444..449; 468..471; 473..475; 491..498; 509..517; 535..537; 539..545

3D Structure

X-ray crystallography (16)

Domain & Motif Annotations

Compositional Bias

21..34; Polar residues; 44..68; Basic and acidic residues; 70..89; Low complexity; 94..130; Basic and acidic residues; 604..622; Low complexity; 623..634; Gly residues

Motif

12..21; Nuclear export signal; 180..208; Q motif; 347..350; DEAD box

Domain (CC)

The C-terminus (residues 536-662) is dispensable for DDX3X trafficking.

Domain (FT)

211..403; Helicase ATP-binding; 414..575; Helicase C-terminal

Region

2..139; Required for TBK1 and IKBKE-dependent IFNB1 activation; 19..144; Disordered; 38..44; Interaction with EIF4E; 81..90; Interaction with VACV protein K7; 88..123; Involved in binding to RNA G-quadruplex; 100..662; Interaction with GSK3B; 100..110; Interaction with IKBKE; 139..172; Interaction with CHUK; 250..259; Involved in stimulation of ATPase activity by DNA and RNA, nucleic acid binding and unwinding and HIV-1 replication; 409..662; Interaction with HCV core protein; 536..661; Interaction with NXF1; 601..634; Disordered

Protein Families

DEAD box helicase family
DDX3/DED1 subfamily

Sequence Similarities

Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.

Clinical Relevance

Disease Involvement

Cancer-related genesDisease variantIntellectual disability

Antibody

HPA001648 HPA005631 HPA059585

Interaction Protein

ENSG00000088888ENSG00000106263ENSG00000114867ENSG00000120948ENSG00000184110ENSG00000263528

Interaction Count

Interaction Dataset

intact_biogrid