Protein detail

PODXL

Podocalyxin (GCTM-2 antigen) (Gp200) (Podocalyxin-like protein 1) (PC) (PCLP-1)

Entry name PODXL	UniProt ID O00592	EVMP confidence score 0.88
Supporting publications (n) 24	Transmembrane count 1	Protein classification Plasma proteinsPredicted membrane proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Podocalyxin (GCTM-2 antigen) (Gp200) (Podocalyxin-like protein 1) (PC) (PCLP-1)

Protein Class (3)

Plasma proteinsPredicted membrane proteinsTransporters

Protein Function

Transporters:Accessory Factors Involved in Transport

Transmembrane

462..482; Helical

Transmembrane Count

Ensembl

ENSG00000128567

Entrez Gene Symbol

PODXL

Gene Synonym (6)

gp135Gp200PCPCLPPDXPODXL1

Gene Description

Podocalyxin like

Chromosome

Position

131500262-131558217

Supporting publications (n)

EVMP confidence score

0.88

Fluorescence & Localization7

Tissue Specificadipose tissueBrain Regional SpecificthalamusCell SpecificEndometrial luminal cellsSingle-Nuclei Brain Specifichippocampal CA4Secretome LocationSecreted to bloodSecretome FunctionNo annotated function

Function & Pathway7

Protein Function

Transporters:Accessory Factors Involved in Transport

Cellular Component (14)

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Molecular Function

GO:0005515 protein binding

Biological Process (3)

KEGG

hsa05132 Salmonella infection

Canonical Pathways (3)

M5880 Naba ecm affiliated
M5885 Naba matrisome associated
M5889 Naba matrisome

Mediation Categories (2)

Adhesion and uptake mediationFusion and delivery mediation

Relations & Evidence35

Ligand-Receptor Signaling (32)

32 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
apical_cell_membrane	plasma_membrane	UniProt_location	No	No	Yes	Yes	No
apical_cell_membrane	plasma_membrane	Ramilowski_location	No	No	Yes	Yes	No
plasma_membrane	plasma_membrane	Ramilowski_location	No	No	Yes	Yes	No
plasma_membrane	plasma_membrane	OmniPath	No	No	Yes	Yes	No
plasma_membrane_transmembrane	plasma_membrane_transmembrane	Membranome	No	No	Yes	Yes	No
plasma_membrane_transmembrane	plasma_membrane_transmembrane	CSPA	No	No	Yes	Yes	No
plasma_membrane_transmembrane	plasma_membrane_transmembrane	OmniPath	No	No	Yes	Yes	No
secreted	secreted	connectomeDB2020	No	No	Yes	Yes	No
secreted	secreted	OmniPath	No	No	Yes	Yes	No
cell_surface	cell_surface	Surfaceome	No	No	Yes	Yes	No

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Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	HSPA12B PODXL	O00592 Q96MM6	0:0	hu.MAP2
	PODXL2 SLC39A12	Q504Y0 Q9NZ53	0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass Spectrometry	2	38113368 41068253

Sequence, Structure & Domains10

Sequences

Length

558

Mass

58,635

Sequence

MRCALALSALLLLLSTPPLLPSSPSPSPSPSQNATQTTTDSSNKTAPTPASSVTIMATDTAQQSTVPTSKANEILASVKATTLGVSSDSPGTTTLAQQVSGPVNTTVARGGGSGNPTTTIESPKSTKSADTTTVATSTATAKPNTTSSQNGAEDTTNSGGKSSHSVTTDLTSTKAEHLTTPHPTSPLSPRQPTSTHPVATPTSSGHDHLMKISSSSSTVAIPGYTFTSPGMTTTLLETVFHHVSQAGLELLTSGDLPTLASQSAGITASSVISQRTQQTSSQMPASSTAPSSQETVQPTSPATALRTPTLPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGDQGPPEEAEDRFSMPLIITIVCMASFLLLVAALYGCCHQRLSQRKDQQRLTEELQTVENGYHDNPTLEVMETSSEMQEKKVVSLNGELGDSWIVPLDNLTKDDLDEEEDTHL

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O00592-1; Sequence=Displayed; Name=2; IsoId=O00592-2; Sequence=VSP_037220

Alternative Sequence

236..268; LETVFHHVSQAGLELLTSGDLPTLASQSAGITA -> P (in isoform 2)

Domain & Motif Annotations

Compositional Bias

20..29; Pro residues; 32..50; Polar residues; 83..107; Polar residues; 125..142; Low complexity; 143..173; Polar residues; 190..204; Polar residues; 270..302; Polar residues; 313..324; Low complexity

Domain (CC)

Both the O-glycan-rich domain of the extracellular domain and the C-terminus PDZ-binding motif (DTHL) in the cytoplasmic tail harbor an apical sorting signal. The cytoplasmic domain is necessary for the apical membrane targeting and renal tubulogenesis. The cytoplasmic C-terminus PDZ-binding motif (DTHL) is essential for interaction with NHERF1 and for targeting NHERF1 to the apical cell membrane. The extracellular domain is necessary for microvillus formation (By similarity). The large highly anionic extracellular domain allows to maintain open filtration pathways between neighboring podocyte foot processes.

Region

20..50; Disordered; 83..210; Disordered; 270..338; Disordered

Protein Families

Podocalyxin family

Sequence Similarities

Belongs to the podocalyxin family.

Clinical Relevance4

Antibody (6)

HPA002110 CAB016169 HPA045507 CAB062558 CAB068219 CAB068220

Interaction Protein

ENSG00000065054

Interaction Count

Interaction Dataset

intact_biogrid_opencell

Supporting Publications24

PMID	Title	Abstract
27894104	Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.	No abstract available
28986585	Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics.	No abstract available
29436780	Autosomal Tubulointerstitial Kidney Disease-MUC1 Type: Differential Proteomics Suggests that Mutated MUC1 (insC) Affects Vesicular Transport in Renal Epithelial Cells.	No abstract available
30646616	Preferential Localization of MUC1 Glycoprotein in Exosomes Secreted by Non-Small Cell Lung Carcinoma Cells.	THBS1, ANXA6, HIST1H4A, COL18A1, MDK, SRGN, ENO1, TUBA4A, SLC3A2, GPI, MIF, MUC1, TALDO1, SLC7A5, ICAM1, HSP90AA1, G6PD, and LRP1 were found to be expressed in exosomes at more than 5-fold higher level as compared to total cellular membrane proteins.
30950185	Unique Protein Profiles of Extracellular Vesicles as Diagnostic Biomarkers for Early and Advanced Non-Small Cell Lung Cancer.	No abstract available
31918306	Potential urinary extracellular vesicle protein biomarkers of chronic active antibody-mediated rejection in kidney transplant recipients.	No abstract available
31941606	The role of actinin-4 (ACTN4) in exosomes as a potential novel therapeutic target in castration-resistant prostate cancer.	No abstract available
32089743	Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism.	No abstract available
32284825	Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine.	No abstract available
32489530	Quantitative proteomic analysis of trypsin-treated extracellular vesicles to identify the real-vesicular proteins.	No abstract available

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