Protein detail

TPSN

Tapasin (TPN) (TPSN) (NGS-17) (TAP-associated protein) (TAP-binding protein)

Entry name TPSN	UniProt ID O15533	EVMP confidence score 0.63
Supporting publications (n) 8	Transmembrane count 1	Protein classification Disease related genesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Tapasin (TPN) (TPSN) (NGS-17) (TAP-associated protein) (TAP-binding protein)

Protein Class (7)

Disease related genesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters

Protein Function (5)

Predicted intracellular proteins
Potential drug targets
Human disease related genes:Immune system diseases:Primary immunodeficiency
Transporters:Accessory Factors Involved in Transport
Disease related genes

Transmembrane

415..435; Helical

Transmembrane Count

Ensembl

ENSG00000231925

Entrez Gene Symbol

TAPBP

Gene Synonym

TAPA

Gene Description

TAP binding protein

Chromosome

Position

33299694-33314284

Supporting publications (n)

EVMP confidence score

0.63

Fluorescence & Localization1

Cell SpecificDecidual stromal cells

Function & Pathway7

Protein Function (5)

Predicted intracellular proteins
Potential drug targets
Human disease related genes:Immune system diseases:Primary immunodeficiency
Transporters:Accessory Factors Involved in Transport
Disease related genes

Cellular Component (8)

Molecular Function (10)

Biological Process (3)

KEGG (5)

Reactome (4)

Mediation Categories (3)

Fusion and delivery mediationImmune mediationMetabolism mediation

Relations & Evidence21

Ligand-Receptor Signaling (17)

17 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
transmembrane	transmembrane	LOCATE	No	No	No	No	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No
transmembrane	transmembrane_predicted	Phobius	No	No	No	No	No
transmembrane_phobius	transmembrane_predicted	Almen2009	No	No	No	No	No
transmembrane_sosui	transmembrane_predicted	Almen2009	No	No	No	No	No
transmembrane_tmhmm	transmembrane_predicted	Almen2009	No	No	No	No	No

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Protein Complex Composition (3)

3 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
TAP complex	ABCB9 TAP1 TAP2 TAPBP	O15533 Q03518 Q03519 Q9NP78	1:1:1:1	Compleat	Compleat:HC739	17947644 15518576 11133832
Tapasin-ERp57 complex	PDIA3 TAPBP	O15533 P30101	2:2	ComplexPortalPDB	intact:EBI-9013963PDB:3f8u	18688235 14755292 14732712
	TAPBP	O15533	2	PDB	PDB:7tuf

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryMass spectrometry [MALDI TOF]Mass spectrometry\|Western blottingWestern blottingMass spectrometry [LTQ-FT Ultra]\|Western blottingMass spectrometry [QTOF]R Sequencing	8	30640524 33497388 33501690 34080172 34507095 35182987 36187097 36187097

Sequence, Structure & Domains11

Sequences

Length

448

Mass

47,571

Sequence

MKSLSLLLAVALGLATAVSAGPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLRPQPEPQQEPVLITMATVVLTVLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEPWGPWTGNGTFWLPTVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSLMPATLARAAPGEAPPELLCLVSHFYPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVTLEVAGLSGPSLEDSVGLFLSAFLLLGLFKALGWAAVYLSTCKDSKKKAE

Alternative Products

Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=O15533-1; Sequence=Displayed; Name=2; IsoId=O15533-2; Sequence=VSP_002577; Name=3; IsoId=O15533-3; Sequence=VSP_017055; Name=4; Synonyms=tpsnDeltaEx3; IsoId=O15533-4; Sequence=VSP_044455

Alternative Sequence

70..156; Missing (in isoform 4); 405..448; LSGPSLEDSVGLFLSAFLLLGLFKALGWAAVYLSTCKDSKKKAE -> KSWELCGI (in isoform 2); 446..448; KAE -> VQCSTSLYLSLVTLSPHPISKPMEGGCWCGRQNLGLEFTLIWVKTWHYILTVGLFEHAT (in isoform 3)

3D Structural Models

Turn

34..36; 306..308

Helix

62..64; 74..81; 104..109; 117..119; 188..191; 263..265; 373..375

Beta Strand

23..29; 41..46; 50..52; 58..60; 65..69; 90..96; 112..116; 123..129; 134..141; 148..150; 153..164; 169..171; 176..178; 181..184; 203..210; 213..220; 236..242; 246..248; 250..258; 267..275; 278..290; 293..300; 302..304; 313..323; 327..337; 345..349; 360..367; 379..385; 394..399

3D Structure

Electron microscopy (2); X-ray crystallography (5)

Domain & Motif Annotations

Domain (CC)

The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminal region.

Domain (FT)

292..399; Ig-like C1-type

Clinical Relevance5

Drugs

ASPIRIN

Antibody (2)

HPA007066 CAB080405

Interaction Protein

ENSG00000168394

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications8

PMID	Title	Abstract
24505114	Proteomics analysis of cancer exosomes using a novel modified aptamer-based array (SOMAscan™) platform.	These included proteins of known association with cancer exosomes such as MFG-E8, integrins, and MET, and also those less widely reported as exosomally associated, such as ROR1 and ITIH4.
26801919	Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis.	No abstract available
31588238	Dual-platform affinity proteomics identifies links between the recurrence of ovarian carcinoma and proteins released into the tumor microenvironment.	No abstract available
36982312	Saliva and Saliva Extracellular Vesicles for Biomarker Candidate Identification-Assay Development and Pilot Study in Amyotrophic Lateral Sclerosis.	No abstract available
38731868	The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer.	No abstract available
39505756	Differential proteomic profiles of exosomes in pediatric and adult adamantinomatous craniopharyngioma cyst fluid.	No abstract available
40098346	Toward Identification of Markers for Brain-Derived Extracellular Vesicles in Cerebrospinal Fluid: A Large-Scale, Unbiased Analysis Using Proximity Extension Assays.	No abstract available
40784529	Serum-derived exosome proteomics unveils the distinct and adjustable nature of the dampness constitution in traditional Chinese medicine.	No abstract available