Protein detail

ASAP2

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 (Development and differentiation-enhancing factor 2) (Paxillin-associated protein with ARF GAP activity 3) (PAG3) (Pyk2 C-terminus-associated protein) (PAP)

Entry name
ASAP2
UniProt ID
EVMP confidence score
0.60
Supporting publications (n)
12
Transmembrane count
Protein classification
Predicted intracellular proteins
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information11
Protein Names
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 (Development and differentiation-enhancing factor 2) (Paxillin-associated protein with ARF GAP activity 3) (PAG3) (Pyk2 C-terminus-associated protein) (PAP)
Protein Class
Predicted intracellular proteins
Protein Function
Predicted intracellular proteins
Entrez Gene Symbol
Gene Synonym (5)
CENTB3DDEF2KIAA0400PAPSHAG1
Gene Description
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Chromosome
2
Position
9206765-9405683
Supporting publications (n)
12
EVMP confidence score
0.60
Fluorescence & Localization3
ASAP2 fluorescence
Tissue SpecificintestineCell SpecificAstrocytes
Function & Pathway6
Relations & Evidence18

Enzyme-Mediated Modification (4)

4 records.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
ASAP2SIK1P57059S701phosphorylationPhosphoNetworks
ASAP2PRKACAP17612S822phosphorylationPhosphoNetworks
ASAP2RPS6KB1P23443S822phosphorylationPhosphoNetworks
ASAP2SRPK2P78362S822phosphorylationPhosphoNetworks

Ligand-Receptor Signaling (7)

7 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
intracellularintracellularLOCATENoNoNoNoNo
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo
plasma_membraneplasma_membraneUniProt_locationNoNoNoNoNo
plasma_membraneplasma_membraneOmniPathNoNoNoNoNo

Regulatory Interaction Network (4)

4 records.

Source Protein SymbolSource UniProt IDTarget Protein SymbolTarget UniProt IDIs DirectedIs StimulationIs InhibitionDatabaseReferences
ASAP2O43150ARF5P84085YesYesNoHPRDSIGNORSIGNOR:10022920HPRD:10749932HPRD:10022920
ASAP2O43150ARF1P84077YesYesYesHPRDWangSIGNORAdhesomeHPRD:10749932Adhesome:11304556Adhesome:10022920SIGNOR:10022920HPRD:10022920Adhesome:10749932
FAK2Q14289ASAP2O43150YesYesNoAdhesomePhosphoPointSIGNORHPRDWangSIGNOR:10022920HPRD:10022920Adhesome:10022920
ASAP2O43150ARF6P62330YesYesNoHPRDWangSIGNORSIGNOR:10022920HPRD:10749932HPRD:10022920

Protein Complex Composition (2)

2 records.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
ASAP2DOK1NCK2WIPF3A6NGB9O43150O43639Q997040:0:0:0hu.MAP
ASAP2CBLBSH3KBP1O43150Q13191Q96B971:1:1CompleatCFinderCompleat:HC3660

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationMass spectrometry131718609
Sequence, Structure & Domains11

Sequences

Length
1,006
Mass
111,651
Sequence
MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINSSGLAHVENEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVEQKEDSQIRQSTAYSLHQPQGNKEHGTERNGSLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPAKLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAKNIGNAGFNEIMECCLPAEDSVKPNPGSDMNARKDYITAKYIERRYARKKHADNAAKLHSLCEAVKTRDIFGLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHYCCLTDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHEDLDESDDDMDEKLQPSPNRREDRPISFYQLGSNQLQSNAVSLARDAANLAKEKQRAFMPSILQNETYGALLSGSPPPAQPAAPSTTSAPPLPPRNVGKVQTASSANTLWKTNSVSVDGGSRQRSSSDPPAVHPPLPPLRVTSTNPLTPTPPPPVAKTPSVMEALSQPSKPAPPGISQIRPPPLPPQPPSRLPQKKPAPGADKSTPLTNKGQPRGPVDLSATEALGPLSNAMVLQPPAPMPRKSQATKLKPKRVKALYNCVADNPDELTFSEGDVIIVDGEEDQEWWIGHIDGDPGRKGAFPVSFVHFIAD
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; Synonyms=PAPalpha; IsoId=O43150-1; Sequence=Displayed; Name=2; Synonyms=PAPbeta; IsoId=O43150-2; Sequence=VSP_009722
Alternative Sequence
795..840; VQTASSANTLWKTNSVSVDGGSRQRSSSDPPAVHPPLPPLRVTSTN -> D (in isoform 2)

Domain & Motif Annotations

Compositional Bias
288..300; Polar residues; 794..823; Polar residues; 865..886; Pro residues
Repeat
584..616; ANK 1; 620..649; ANK 2
Coiled Coil
256..283; 729..752
Domain (CC)
The conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.
Domain (FT)
305..397; PH; 421..543; Arf-GAP; 944..1006; SH3
Region
288..312; Disordered; 701..720; Disordered; 764..916; Disordered
Clinical Relevance4
Interaction Protein (2)
ENSG00000147010ENSG00000177885
Interaction Count
2
Interaction Dataset
intact_biogrid
Supporting Publications12
PMIDTitleAbstract
27605433Secreted primary human malignant mesothelioma exosome signature reflects oncogenic cargo.No abstract available
28986585Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics.No abstract available
29148239Metabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.No abstract available
32089743Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism.No abstract available
34817906Proteomic dissection of large extracellular vesicle surfaceome unravels interactive surface platform.No abstract available
36398564Differential ultracentrifugation enables deep plasma proteomics through enrichment of extracellular vesicles.No abstract available
37786918Rapid and in-depth proteomic profiling of small extracellular vesicles for ultralow samples.No abstract available
38321535Identification of specific markers for human pluripotent stem cell-derived small extracellular vesicles.No abstract available
39766158A Proteomic Examination of Plasma Extracellular Vesicles Across Colorectal Cancer Stages Uncovers Biological Insights That Potentially Improve Prognosis.No abstract available
39940641Disease-Associated Signatures Persist in Extracellular Vesicles from Reprogrammed Cells of Osteoarthritis Patients.No abstract available
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