Protein detail

PEPL

Periplakin (190 kDa paraneoplastic pemphigus antigen) (195 kDa cornified envelope precursor protein)

Entry name PEPL	UniProt ID O60437	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information10

Protein Names

Periplakin (190 kDa paraneoplastic pemphigus antigen) (195 kDa cornified envelope precursor protein)

Protein Class (2)

Plasma proteinsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000118898

Entrez Gene Symbol

PPL

Gene Description

Periplakin

Chromosome

Position

4882507-4960741

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization2

Cell SpecificcDCSingle-Nuclei Brain Specificcentral nervous system macrophage

Function & Pathway7

Protein Function

Predicted intracellular proteins

Cellular Component (9)

Molecular Function (4)

Biological Process (3)

KEGG

hsa04382 Cornified envelope formation

Reactome (4)

Mediation Categories (2)

Immune mediationMetabolism mediation

Relations & Evidence29

Enzyme-Mediated Modification (2)

2 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
PPL	ATM	Q13315	S	1,331	phosphorylation	KEA	KEA:17570479
PPL	PRKDC	P78527	S	1,331	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (9)

9 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	GO_Intercell	Yes	No	No	No	No
ecm	ecm	OmniPath	Yes	No	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Protein Complex Composition (17)

17 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ETNPPL GBE1 UGDH	O60701 Q04446 Q8TBG4	0:0:0	hu.MAP2
MYBPC2 MYL11 MYOM1 RIPPLY1 RIPPLY2 RIPPLY3 TBX20 TLE1 TLE2 TLE3 TLE4 TLE5	P52179 P57055 Q04724 Q04725 Q04726 Q04727 Q08117 Q0D2K3 Q14324 Q5TAB7 Q96A32 Q9UMR3	0:0:0:0:0:0:0:0:0:0:0:0	hu.MAP2
RIPPLY1 RIPPLY2 RIPPLY3 TLE1 TLE2 TLE4 TLE5	P57055 Q04724 Q04725 Q04727 Q08117 Q0D2K3 Q5TAB7	0:0:0:0:0:0:0	hu.MAP2
ETNPPL GBE1	Q04446 Q8TBG4	0:0	hu.MAP2
RIPPLY1 RIPPLY2 TLE2	Q04725 Q0D2K3 Q5TAB7	0:0:0	hu.MAP2
APPL2	Q8NEU8	4	PDB	PDB:4h8s
ETNPPL	Q8TBG4	2	PDB	PDB:6tor

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow Filtration	Mass spectrometryR Sequencing	1	28396511

Sequence, Structure & Domains13

Sequences

Length

1,756

Mass

204,747

Sequence

MNSLFRKRNKGKYSPTVQTRSISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQGLISRGYSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEVLKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQSWELLATHENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASREQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRRSHVSKRARLQSPATKVKEEEAALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLENEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELEALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAEVLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDEAAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTRLIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQKKQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELRQIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDSRLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVNKDMSIQELAVLVSGQK

3D Structural Models

Turn

1663..1665; 1713..1716

Helix

1671..1676; 1682..1689; 1722..1726; 1732..1739; 1745..1753

Beta Strand

1655..1661; 1693..1701; 1706..1712; 1717..1721

3D Structure

X-ray crystallography (1)

Domain & Motif Annotations

Repeat

216..317; Spectrin 1; 323..485; Spectrin 2; 505..612; Spectrin 3; 733..861; Spectrin 4; 1651..1685; Plectin 1; 1700..1735; Plectin 2

Coiled Coil

16..125; 188..389; 585..820; 886..1645

Domain (FT)

399..455; SH3

Region

1557..1756; Interacts with BFSP2 and VIM

Protein Families

Plakin or cytolinker family

Sequence Similarities

Belongs to the plakin or cytolinker family.

Clinical Relevance4

Antibody (2)

HPA042550 HPA059859

Interaction Protein (3)

ENSG00000017797ENSG00000026025ENSG00000142208

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications1

PMID	Title	Abstract
30550287	Label-Free Proteomic Analysis of Exosomes Secreted from THP-1-Derived Macrophages Treated with IFN-α Identifies Antiviral Proteins Enriched in Exosomes.	No abstract available