Protein detail

GLRA3

Glycine receptor subunit alpha-3

Entry name
GLRA3
UniProt ID
EVMP confidence score
0.50
Supporting publications (n)
1
Transmembrane count
4
Protein classification
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information8
Protein Names
Glycine receptor subunit alpha-3
Protein Function (2)
  • Transporters:Transporter channels and pores
  • FDA approved drug targets:Small molecule drugs
Transmembrane
256..277; Helical; Name=1; 283..303; Helical; Name=2; 315..335; Helical; Name=3; 431..451; Helical; Name=4
Transmembrane Count
4
Entrez Gene Symbol
Supporting publications (n)
1
EVMP confidence score
0.50
Fluorescence & Localization1
Cell SpecificEndometrial ciliated cells
Function & Pathway8
Protein Function (2)
  • Transporters:Transporter channels and pores
  • FDA approved drug targets:Small molecule drugs
Canonical Pathways (3)
  • M137 Pid vegf vegfr pathway
  • M226 Pid vegfr1 pathway
  • M33 Pid glypican 1pathway
Mediation Categories
Fusion and delivery mediation
Relations & Evidence35

Ligand-Receptor Signaling (32)

32 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
gaba_achreceptorAlmen2009NoYesNoNoNo
receptorreceptorOmniPathNoYesNoNoNo
extracellularextracellularOmniPathNoNoNoNoNo
intracellularintracellularLOCATENoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo
transportertransporterSurfaceomeNoYesNoNoNo
cys_looptransporterSurfaceomeNoYesNoNoNo
channelstransporterSurfaceomeNoYesNoNoNo
ion_channelion_channelDGIdbNoYesNoNoNo
Page 1 of 4Next

Protein Complex Composition (2)

2 records.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
Glycine receptor complexGLRA3-GLRBGLRA3GLRBO75311P481674:1ComplexPortalPDBPDB:9bozintact:EBI-44438063PDB:9boyPDB:9bp0PDB:9bp7147552923601655735526563
GLRA3O753115PDBPDB:5vdhPDB:5tinPDB:5tioPDB:9bvhPDB:9bwePDB:9bzpPDB:9bvjPDB:9bwgPDB:9bu2PDB:9bwjPDB:9bwbPDB:5cfbPDB:9bwcPDB:5vdiPDB:9bu3

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationSize Exclusion ChromatographyWestern blotting51849072436335434363748193649950136931723
Sequence, Structure & Domains12

Sequences

Length
464
Mass
53,800
Sequence
MAHVRHFRTLVSGFYFWEAALLLSLVATKETDSARSRSAPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSIAETTMDYRVNIFLRQKWNDPRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGANFHEVTTDNKLLRIFKNGNVLYSIRLTLTLSCPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQDEAPVQVAEGLTLPQFLLKEEKDLRYCTKHYNTGKFTCIEVRFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRKNKTEAFALEKFYRFSDMDDEVRESRFSFTAYGMGPCLQAKDGMTPKGPNHPVQVMPKSPDEMRKVFIDRAKKIDTISRACFPLAFLIFNIFYWVIYKILRHEDIHQQQD
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=Alpha-3L; IsoId=O75311-1; Sequence=Displayed; Name=Alpha-3K; IsoId=O75311-2; Sequence=VSP_000084
Alternative Sequence
358..372; Missing (in isoform Alpha-3K)

3D Structural Models

Turn
53..56; 86..89; 196..198; 304..306
Helix
43..50; 104..106; 120..125; 178..180; 254..259; 261..272; 273..276; 282..303; 315..341; 420..452; 454..456
Beta Strand
63..68; 70..85; 90..102; 110..112; 114..118; 131..133; 136..141; 144..146; 148..154; 157..170; 182..193; 199..203; 208..211; 218..222; 227..230; 232..235; 238..240; 242..250
3D Structure
Electron microscopy (14); X-ray crystallography (5)

Domain & Motif Annotations

Domain (CC)
The N-terminal domain carries structural determinants essential for agonist and antagonist binding. The channel pore is formed by pentameric assembly of the second transmembrane domain from all five subunits (PubMed:26416729). The cytoplasmic loop is an important determinant of channel inactivation kinetics.
Protein Families (3)
  • Ligand-gated ion channel (TC 1.A.9) family
  • Glycine receptor (TC 1.A.9.3) subfamily
  • GLRA3 sub-subfamily
Sequence Similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA3 sub-subfamily.
Clinical Relevance1
Supporting Publications1
PMIDTitleAbstract
34265469Proteomic Landscape of Exosomes Reveals the Functional Contributions of CD151 in Triple-Negative Breast Cancer.Furthermore, utilizing quantitative proteomics approach to reveal the proteomes of CD151-deleted exosomes and cells, we found that exosomal CD151 facilitated secretion of ribosomal proteins via exosomes while inhibiting exosome secretion of complement proteins. Moreover, we proved that CD151-deleted exosomes significantly decreased the migration and invasion of TNBC cells. Most importantly, we found that the tetraspanin CD151 expression levels in TNBC-derived serum exosomes were significantly higher than those exosomes from healthy subjects, and we validated our findings with samples from 16 additional donors. This is the first comparative study of the proteomes of TNBC patient-derived and CD151-deleted exosomes.