EV MemProt Atlas

Basic Information13

Protein Names

Integrin beta-3 (Platelet membrane glycoprotein IIIa) (GPIIIa) (CD antigen CD61)

Protein Class (8)

Cancer-related genesCD markersDisease related genesFDA approved drug targetsHuman disease related genesPlasma proteinsPredicted intracellular proteinsPredicted membrane proteins

Protein Function (9)

Human disease related genes:Immune system diseases:Allergies and autoimmune diseases
Human disease related genes:Cardiovascular diseases:Hematologic diseases
Predicted intracellular proteins
CD markers
Cancer-related genes:Candidate cancer biomarkers
Disease related genes
Human disease related genes:Cardiovascular diseases:Cardiac diseases
FDA approved drug targets:Small molecule drugs
FDA approved drug targets:Biotech drugs

Transmembrane

719..741; Helical

Transmembrane Count

1

Ensembl

ENSG00000259207

Entrez Gene Symbol

ITGB3

Gene Synonym (3)

CD61GP3AGPIIIa

Gene Description

Integrin subunit beta 3

Chromosome

17

Position

47253827-47313743

Supporting publications (n)

6

EVMP confidence score

0.63

Fluorescence & Localization4

Cell SpecificAlveolar cells type 1Single-Nuclei Brain Specificendothelial cellBlood Cell SpecificNK-cellBlood Lineage SpecificNK-cells

Function & Pathway7

Protein Function (9)

Human disease related genes:Immune system diseases:Allergies and autoimmune diseases
Human disease related genes:Cardiovascular diseases:Hematologic diseases
Predicted intracellular proteins
CD markers
Cancer-related genes:Candidate cancer biomarkers
Disease related genes
Human disease related genes:Cardiovascular diseases:Cardiac diseases
FDA approved drug targets:Small molecule drugs
FDA approved drug targets:Biotech drugs

Cellular Component (29)

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Molecular Function (20)

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Biological Process (3)

KEGG (26)

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Reactome (35)

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Mediation Categories (6)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence112

Enzyme-Mediated Modification (16)

16 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
ITGB3	PDPK1	O15530	T	777	phosphorylation	phosphoELMKEA	KEA:10896934 phosphoELM:10896934
ITGB3	PDPK1	O15530	T	779	phosphorylation	SIGNORProtMapperHPRDReactome_ProtMapperdbPTMKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	HPRD:18578522 SIGNOR:10896934 HPRD:10896934 phosphoELM:10896934 dbPTM:10896934 KEA:10896934 ProtMapper:10896934
ITGB3	SRC	P12931	Y	785	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperHPRDKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:8631894 HPRD:18088087 KEA:18088087 ProtMapper:11723131 phosphoELM:8631894 KEA:11723131 HPRD:11723131
ITGB3	SRC	P12931	Y	773	phosphorylation	BEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEAphosphoELMLi2012SIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:15699036 ProtMapper:15212693 SIGNOR:11723131 KEA:8631894 HPRD:18088087 KEA:18088087 ProtMapper:11723131 phosphoELM:18398066 phosphoELM:15699036 KEA:11723131 HPRD:11723131
ITGB3	SYK	P43405	Y	785	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEAphosphoELM	KEA:8631894 KEA:11723131 KEA:18088087 phosphoELM:8631894
ITGB3	SYK	P43405	Y	773	phosphorylation	Li2012KEA	KEA:8631894 KEA:11723131 KEA:15699036 KEA:18088087
ITGB3	AKT1	P31749	T	779	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDReactome_ProtMapperdbPTMKEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	HPRD:18578522 SIGNOR:10896934 HPRD:10896934 dbPTM:10896934 KEA:10896934 ProtMapper:10896934
ITGB3	PDK1	Q15118	T	779	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEASIGNOR_ProtMapper	HPRD:18578522 SIGNOR:10896934 HPRD:10896934 KEA:10896934 ProtMapper:10896934
ITGB3	PDK1	Q15118	T	777	phosphorylation	MIMPKEAphosphoELM_MIMPPhosphoSite_MIMP	KEA:10896934
ITGB3	CAPN1	P07384	A	761	cleavage	HPRD	HPRD:7592818

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Ligand-Receptor Signaling (70)

70 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
integrin	receptor	Integrins	No	Yes	No	Yes	No
integrin	receptor	UniProt_keyword	No	Yes	No	Yes	No
integrins	receptor	HPMR	No	Yes	No	Yes	No
itb_4	receptor	HPMR	No	Yes	No	Yes	No
integrin	receptor	Surfaceome	No	Yes	No	Yes	No
cell_adhesion	receptor	ICELLNET	No	Yes	No	Yes	No
ecm_interaction	receptor	ICELLNET	No	Yes	No	Yes	No
ecm_interaction_fn1	receptor	ICELLNET	No	Yes	No	Yes	No
receptor	receptor	OmniPath	No	Yes	No	Yes	No
extracellular	extracellular	HPMR	No	No	No	Yes	No

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Regulatory Interaction Network (10)

10 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
DOK1	Q99704	ITB3	P05106	Yes	No	Yes	ELMLMPIDSIGNORHPRD	SIGNOR:19118207 LMPID:12606711 ELM:20739287 ELM:18156175 HPRD:12606711 ELM:12606711 LMPID:20739287 LMPID:18156175
SRC	P12931	ITB3	P05106	Yes	Yes	Yes	HPRD_MIMPSIGNORProtMapperHINTPhosphoSite_KEAphosphoELM_KEALi2012HPRDWangPhosphoSite_ProtMapperBEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSiteAdhesomeHPRD-phos	PhosphoSite:16935858 KEA:15699036 PhosphoSite:8631894 SIGNOR:11723131 PhosphoSite:12359711 PhosphoSite:18156175 HPRD-phos:18088087 phosphoELM:18398066 phosphoELM:15699036 PhosphoSite:15695822 PhosphoSite:15699036 PhosphoSite:20702409 PhosphoSite:34534368 ProtMapper:15212693 phosphoELM:8631894 PhosphoSite:17473008 ProtMapper:18088087 PhosphoSite:10330414 Adhesome:10592173 HINT:10896934 KEA:8631894 KEA:18088087 Adhesome:18840695 PhosphoSite:14996908 PhosphoSite:11723131 HINT:23169783 PhosphoSite:18398066 PhosphoSite:18364349 PhosphoSite:25694433 ProtMapper:11723131 Adhesome:17353363 KEA:11723131 HPRD:11723131 PhosphoSite:21956114 HPRD-phos:11723131
RGS6	P49758	ITB3	P05106	Yes	No	Yes	SIGNOR	SIGNOR:17609107
PDK1	Q15118	ITB3	P05106	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperPhosphoSite_KEAKEAphosphoELM_KEAHPRD_KEASIGNOR_ProtMapperHPRD-phos	ProtMapper:18578522 SIGNOR:10896934 HPRD-phos:18578522 KEA:10896934 HPRD-phos:10896934 ProtMapper:10896934
AKT1	P31749	ITB3	P05106	Yes	Yes	Yes	HPRD_MIMPSIGNORProtMapperdbPTMPhosphoSite_KEAHPRDWangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEASIGNOR_ProtMapperAdhesomeReactome_ProtMapperHPRD-phos	ProtMapper:18578522 SIGNOR:10896934 HPRD-phos:18578522 HPRD-phos:10896934 HPRD:10896934 KEA:10896934 dbPTM:10896934 Adhesome:10896934 ProtMapper:10896934
UPAR	Q03405	ITB3	P05106	Yes	Yes	No	WangSIGNOR	SIGNOR:27383564
TLN1	Q9Y490	ITB3	P05106	Yes	Yes	No	WangCellTalkDBAdhesomeSIGNORReactome_LRdbELMCui2007HINTHPRDIntActLit-BM-17SPIKE_LCLRdb	HPRD:11932255 Adhesome:16546176 IntAct:16546176 SPIKE_LC:16546176 HPRD:10497223 Lit-BM-17:10497223 SPIKE_LC:12535520 CellTalkDB:32196115 Lit-BM-17:16546176 HINT:10497223 Adhesome:10497223 SIGNOR:19118207 ELM:12606711 ELM:12535520 Adhesome:11932255 HINT:16546176 HINT:11412103 HINT:11932255 Adhesome:17218263 Adhesome:12606711 ELM:20739287 ELM:18156175 HPRD:12606711 ELM:9065427 Lit-BM-17:11932255 HINT:19843520
URP2	Q86UX7	ITB3	P05106	Yes	Yes	No	SIGNOR	SIGNOR:18278053
ITBP1	O14713	ITB3	P05106	Yes	No	Yes	SIGNOR	SIGNOR:19118207
PDPK1	O15530	ITB3	P05106	Yes	Yes	Yes	WangAdhesomeiPTMnetPhosphoPointSIGNORProtMapperHPRDReactome_ProtMapperPhosphoSite_KEAKEAdbPTMHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSiteHPRD-phosPhosphoSite_ProtMapper	PhosphoSite:10896934 ProtMapper:18578522 SIGNOR:10896934 HPRD-phos:18578522 HPRD-phos:10896934 HPRD:10896934 KEA:10896934 phosphoELM:10896934 dbPTM:10896934 Adhesome:10896934 ProtMapper:10896934

Protein Complex Composition (15)

15 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
CCAN complex	CENPC CENPH CENPI CENPK CENPL CENPM CENPN CENPO CENPP CENPQ CENPS CENPT CENPU CENPW CENPX ITGB3BP	A8MT69 Q03188 Q13352 Q5EE01 Q6IPU0 Q71F23 Q7L2Z9 Q8N0S6 Q8N2Z9 Q92674 Q96BT3 Q96H22 Q9BS16 Q9BU64 Q9H3R5 Q9NSP4	1:2:1:1:1:1:1:1:1:1:1:1:1:2:1:1	SIGNORComplexPortalPDB	PDB:7xhointact:EBI-25469601PDB:4DRASIGNOR:SIGNOR-C365PDB:7xhnPDB:4NE3	36085283 18007590 14755292 18097444
CENP-A NAC-CAD complex	CENPC CENPH CENPK CENPL CENPM CENPN CENPO CENPP CENPQ CENPS CENPT CENPU ITGB3BP	Q03188 Q13352 Q6IPU0 Q71F23 Q7L2Z9 Q8N0S6 Q8N2Z9 Q96BT3 Q96H22 Q9BS16 Q9BU64 Q9H3R5 Q9NSP4	1:1:1:1:1:1:1:1:1:1:1:1:1	CompleatCORUM	Compleat:HC3611CORUM:1179	16622419
CENP-H-I complex	CENPH CENPI CENPK CENPL CENPM CENPN CENPO CENPP CENPQ CENPU ITGB3BP	Q13352 Q6IPU0 Q71F23 Q7L2Z9 Q8N0S6 Q92674 Q96H22 Q9BS16 Q9BU64 Q9H3R5 Q9NSP4	1:1:1:1:1:1:1:1:1:1:1	KEGG-MEDICUSCORUMPDB	PDB:7r5vPDB:7pknCORUM:7394	16622420
	CENPA CENPC CENPH CENPI CENPK CENPL CENPM CENPN CENPO CENPP CENPQ CENPS CENPT CENPU CENPW CENPX H2AC6 H2BC7 H4C4 ITGB3BP	A8MT69 P49450 P62805 P62807 Q03188 Q13352 Q5EE01 Q6IPU0 Q71F23 Q7L2Z9 Q8N0S6 Q8N2Z9 Q92674 Q93077 Q96BT3 Q96H22 Q9BS16 Q9BU64 Q9H3R5 Q9NSP4	1:2:2:2:2:1:1:1:1:1:1:1:1:2:1:1:1:1:1:1	PDB	PDB:7ywx
	CENPH CENPI CENPK CENPL CENPM CENPN CENPO CENPP CENPQ CENPS CENPT CENPU CENPW CENPX ITGB3BP	A8MT69 Q13352 Q5EE01 Q6IPU0 Q71F23 Q7L2Z9 Q8N0S6 Q8N2Z9 Q92674 Q96BT3 Q96H22 Q9BS16 Q9BU64 Q9H3R5 Q9NSP4	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	PDB	PDB:7r5s
	CENPH CENPI CENPK CENPM CENPN CENPO CENPP CENPQ CENPT CENPU CENPX ITGB3BP	A8MT69 Q13352 Q6IPU0 Q71F23 Q7L2Z9 Q92674 Q96BT3 Q96H22 Q9BS16 Q9BU64 Q9H3R5 Q9NSP4	0:0:0:0:0:0:0:0:0:0:0:0	hu.MAP
	ITGB3	P05106	2	PDB	PDB:6bxfPDB:6ckbPDB:6bxb
	ITGB3 SHC1	P05106 P29353	1:1	PDB	PDB:2l1c
	CENPA CENPC CENPH CENPI CENPK CENPL CENPM CENPN CENPO CENPP CENPQ CENPU H2AC6 H2BC7 H4C4 ITGB3BP	P49450 P62805 P62807 Q03188 Q13352 Q6IPU0 Q71F23 Q7L2Z9 Q8N0S6 Q92674 Q93077 Q96H22 Q9BS16 Q9BU64 Q9H3R5 Q9NSP4	2:2:2:2:1:1:1:1:1:1:2:1:1:1:1:1	PDB	PDB:7yyh
	CENPC CENPH CENPI CENPK CENPL CENPM CENPN CENPO CENPP CENPQ CENPT CENPU CENPW ITGB3BP	Q03188 Q13352 Q5EE01 Q6IPU0 Q71F23 Q7L2Z9 Q8N0S6 Q92674 Q96BT3 Q96H22 Q9BS16 Q9BU64 Q9H3R5 Q9NSP4	1:1:1:1:1:1:1:1:1:1:1:1:1:1	PDB	PDB:7qoo

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass Spectrometry	2	38113368 41068253

Sequence, Structure & Domains15

Sequences

Length

788

Mass

87,058

Sequence

MRARPRPRPLWATVLALGALAGVGVGGPNICTTRGVSSCQQCLAVSPMCAWCSDEALPLGSPRCDLKENLLKDNCAPESIEFPVSEARVLEDRPLSDKGSGDSSQVTQVSPQRIALRLRPDDSKNFSIQVRQVEDYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPVSPYMYISPPEALENPCYDMKTTCLPMFGYKHVLTLTDQVTRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVQPNDGQCHVGSDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTENVVNLYQNYSELIPGTTVGVLSMDSSNVLQLIVDAYGKIRSKVELEVRDLPEELSLSFNATCLNNEVIPGLKSCMGLKIGDTVSFSIEAKVRGCPQEKEKSFTIKPVGFKDSLIVQVTFDCDCACQAQAEPNSHRCNNGNGTFECGVCRCGPGWLGSQCECSEEDYRPSQQDECSPREGQPVCSQRGECLCGQCVCHSSDFGKITGKYCECDDFSCVRYKGEMCSGHGQCSCGDCLCDSDWTGYYCNCTTRTDTCMSSNGLLCSGRGKCECGSCVCIQPGSYGDTCEKCPTCPDACTFKKECVECKKFDRGALHDENTCNRYCRDEIESVKELKDTGKDAVNCTYKNEDDCVVRFQYYEDSSGKSILYVVEEPECPKGPDILVVLLSVMGAILLIGLAALLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=Beta-3A; IsoId=P05106-1; Sequence=Displayed; Name=Beta-3B; IsoId=P05106-2; Sequence=VSP_002745; Name=Beta-3C; IsoId=P05106-3; Sequence=VSP_002746

Alternative Sequence

768..788; ANNPLYKEATSTFTNITYRGT -> VRDGAGRFLKSLV (in isoform Beta-3B); 768..788; ANNPLYKEATSTFTNITYRGT -> HYAQSLRKWNQPVSIDG (in isoform Beta-3C)

3D Structural Models

Turn

157..159; 169..171; 188..190; 202..207; 308..312; 361..363; 401..403; 472..474; 475..478; 494..498; 616..618; 686..688; 743..759; 761..765; 782..786

Helix

30..33; 35..37; 39..45; 67..72; 77..79; 103..105; 148..150; 151..156; 160..168; 196..200; 226..235; 248..257; 259..262; 285..289; 318..327; 338..340; 341..350; 366..377; 462..466; 509..511; 520..523; 562..564; 591..593; 601..603; 633..645; 650..653; 657..660; 720..737; 771..774; 776..779

Beta Strand

49..52; 55..57; 59..61; 63..66; 86..91; 97..101; 109..111; 112..118; 123..131; 138..145; 175..182; 215..224; 242..246; 268..278; 305..307; 330..336; 355..358; 381..387; 392..400; 404..407; 410..414; 420..429; 434..444; 451..457; 468..470; 479..482; 485..488; 489..491; 500..504; 505..508; 512..518; 524..528; 531..534; 538..540; 542..544; 549..552; 556..561; 565..569; 572..575; 579..581; 598..600; 604..608; 611..614; 620..624; 628..630; 663..670; 676..684; 690..697; 698..700; 703..710; 715..717; 767..770

3D Structure

Electron microscopy (25); NMR spectroscopy (17); X-ray crystallography (72)

Domain & Motif Annotations

Motif

777..783; LIR

Domain (CC)

The VWFA domain (or beta I domain) contains three cation-binding sites: the ligand-associated metal ion-binding site (LIMBS or SyMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent MIDAS site (ADMIDAS). This domain is also part of the ligand-binding site.

Domain (FT)

30..76; PSI; 135..377; VWFA; 463..498; I-EGF 1; 499..548; I-EGF 2; 549..585; I-EGF 3; 586..625; I-EGF 4

Region

203..210; Involved in CX3CL1-, NRG1-, FGF1- and IGF1-binding; 293..313; CX3CL1-binding

Protein Families

Integrin beta chain family

Sequence Similarities

Belongs to the integrin beta chain family.

Clinical Relevance7

Disease Involvement (3)

Cancer-related genesDisease variantFDA approved drug targets

Drug Targets (4)

FDA approved drug targetsClinical trial targetSuccessful targetLiterature-reported target

Drugs (16)

CYPATE CILENGITIDE TIROFIBAN HYDROCHLORIDE ZALUNFIBAN TIROFIBAN TADOCIZUMAB ABCIXIMAB ABITUZUMAB INTEGRIN RECEPTOR ANTAGONIST GLPG0187 EPTIFIBATIDE INTETUMUMAB ATN-161 ANTI-THYMOCYTE GLOBULIN ETARACIZUMAB NULL GANTOFIBAN

Antibody

HPA027852

Interaction Protein (3)

ENSG00000005961ENSG00000137076ENSG00000138448

Interaction Count

3

Interaction Dataset

intact_biogrid

Supporting Publications6

PMID	Title	Abstract
32848136	ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells.	The functional requirement for ITGB3 derives from its interactions with heparan sulfate proteoglycans (HSPGs) and the process of integrin endocytosis, allowing the capture of extracellular vesicles and their endocytosis-mediated internalization. Thus, ITGB3 has a central role in intracellular communication via extracellular vesicles, proposed to be critical for cancer metastasis.
34684727	Proteomic Signature of Extracellular Vesicles for Lung Cancer Recognition.	By applying targeted mass spectrometry with stable isotope-labeled peptide standards, we assessed the levels of 28 EV-associated proteins, including the conventional exosome markers CD9, CD63, CD81, CD82, and HSPA8, in vesicles derived from the lung cancer cell lines NCI-H23 and A549.
36263165	Platelet-derived extracellular vesicles inhibit ferroptosis and promote distant metastasis of nasopharyngeal carcinoma by upregulating ITGB3.	Here we found that the distant metastasis of NPC patients was positively correlated with the expression levels of integrin β3 (ITGB3) in platelet-derived extracellular vesicles (EVs) from NPC patients (P-EVs).
37040507	Single-Exosome Profiling Identifies ITGB3+ and ITGAM+ Exosome Subpopulations as Promising Early Diagnostic Biomarkers and Therapeutic Targets for Colorectal Cancer.	On the contrary, ITGAM-positive exosomes show a large-scale increase in plasma of HC group, compared to both primary CRC and metastatic CRC groups.
39136603	ITGB3-enriched extracellular vesicles mediate the formation of osteoclastic pre-metastatic niche to promote lung adenocarcinoma bone metastasis.	Extracellular vesicles (EVs) isolated from the conditioned medium (CM) of LUAD cells overexpressing ITGB3 determined that ITGB3 facilitates osteoclastogenesis and enhances osteoclast activity by utilizing EVs-mediated transport to RAW264.7 cells.
39229506	Exosomal CTHRC1 from cancer-associated fibroblasts facilitates endometrial cancer progression via ITGB3/FAK signaling pathway.	Overexpression of CTHRC1 in secreted exosomes promotes the metastatic ability of EC cells in mouse models and may be eliminated by Defactinib, an inhibitor of FAK Tyr397 phosphorylation. Quantitative proteomics analysis of CAF/NF-derived exosomes demonstrated differential expression of CTHRC1, a protein overexpressed in multiple tumors, promoting cancer progression through enhanced cell migration and invasion.

Entry name ITB3	UniProt ID P05106	EVMP confidence score 0.63
Supporting publications (n) 6	Transmembrane count 1	Protein classification Cancer-related genesCD markersDisease related genesFDA approved drug targetsHuman disease related genesPlasma proteinsPredicted intracellular proteinsPredicted membrane proteins

ITB3