Protein detail

PPBT

Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP) (TNS-ALP) (TNSALP) (EC 3.1.3.1) (Alkaline phosphatase liver/bone/kidney isozyme) (Phosphoamidase) (Phosphocreatine phosphatase) (EC 3.9.1.1)

Entry name PPBT	UniProt ID P05186	EVMP confidence score 0.47
Supporting publications (n) 14	Transmembrane count	Protein classification Cancer-related genesDisease related genesEnzymesHuman disease related genesMetabolic proteinsPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP) (TNS-ALP) (TNSALP) (EC 3.1.3.1) (Alkaline phosphatase liver/bone/kidney isozyme) (Phosphoamidase) (Phosphocreatine phosphatase) (EC 3.9.1.1)

Protein Class (9)

Cancer-related genesDisease related genesEnzymesHuman disease related genesMetabolic proteinsPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

Protein Function (7)

Human disease related genes:Congenital disorders of metabolism:Congenital disorders of cofactor/vitamin metabolism
Predicted intracellular proteins
Potential drug targets
Enzymes
Cancer-related genes:Candidate cancer biomarkers
ENZYME proteins:Hydrolases
Disease related genes

Ensembl

ENSG00000162551

Entrez Gene Symbol

ALPL

Gene Synonym (4)

HOPSTNALPTNAPTNSALP

Gene Description

Alkaline phosphatase, biomineralization associated

Chromosome

Position

21509397-21578410

Supporting publications (n)

EVMP confidence score

0.47

Fluorescence & Localization2

Tissue SpecifictestisCell SpecificEarly primary spermatocytes

Function & Pathway7

Protein Function (7)

Human disease related genes:Congenital disorders of metabolism:Congenital disorders of cofactor/vitamin metabolism
Predicted intracellular proteins
Potential drug targets
Enzymes
Cancer-related genes:Candidate cancer biomarkers
ENZYME proteins:Hydrolases
Disease related genes

Cellular Component (8)

Molecular Function (9)

Biological Process (3)

KEGG (4)

Reactome (2)

Mediation Categories (3)

Clinical-translation mediationImmune mediationMetabolism mediation

Relations & Evidence25

Ligand-Receptor Signaling (19)

19 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	MatrixDB	Yes	No	No	No	Yes
ecm	ecm	CellCellInteractions	Yes	No	No	No	Yes
ecm	ecm	OmniPath	Yes	No	No	No	Yes
extracellular	extracellular	OmniPath	No	No	No	No	Yes
intracellular	intracellular	LOCATE	No	No	No	No	Yes
intracellular	intracellular	ComPPI	No	No	No	No	Yes
intracellular	intracellular	GO_Intercell	No	No	No	No	Yes
intracellular	intracellular	UniProt_location	No	No	No	No	Yes
intracellular	intracellular	OmniPath	No	No	No	No	Yes
transmembrane	transmembrane	Ramilowski_location	No	No	No	No	Yes

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Regulatory Interaction Network (2)

2 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
PPBT	P05186	TAU	P10636	Yes	No	Yes	SIGNOR	SIGNOR:24646911
PPBT	P05186	OSTP	P10451	Yes	No	Yes	SIGNOR	SIGNOR:23427088

Protein Complex Composition (3)

3 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ALPL EIF3A EIF3B EIF3F EIF3H EIF3I EIF3J EIF4A1 EIF4A2 EIF4E EIF4G1 EIF4G2 EIF4G3 PABPC1 SUMO1	O00303 O15372 O43432 O75822 P05186 P06730 P11940 P55884 P60842 P63165 P78344 Q04637 Q13347 Q14152 Q14240	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7035
ALPL	P05186	8	PDB	PDB:7yiwPDB:7yixPDB:7yiv
ALPL BIRC3 BIRC6 BIRC7 CASP9 CDC23	P05186 P55211 Q13489 Q96CA5 Q9NR09 Q9UJX2	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9558

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Western blottingFlow cytometryMORPH	3	32218180 33709510 32089743

Sequence, Structure & Domains12

Sequences

Length

524

Mass

57,305

Sequence

MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVLF

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=P05186-1; Sequence=Displayed; Name=2; IsoId=P05186-2; Sequence=VSP_042711; Name=3; IsoId=P05186-3; Sequence=VSP_044228

Alternative Sequence

1..99; MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSK -> MPWSFRSSTPTWLRMSSCSWEM (in isoform 2); 1..55; Missing (in isoform 3)

3D Structural Models

Turn

88..91; 176..178; 189..191; 303..305; 404..406

Helix

21..24; 26..42; 65..77; 111..119; 139..141; 150..156; 172..175; 194..198; 204..210; 252..257; 272..277; 280..282; 298..300; 309..320; 335..342; 345..365; 431..433; 468..470; 477..479; 480..487; 495..497

Beta Strand

52..59; 93..99; 103..107; 128..130; 160..168; 217..221; 224..228; 240..243; 246..248; 284..289; 291..294; 327..333; 370..378; 383..385; 411..418; 445..448; 459..465; 473..476

3D Structure

Electron microscopy (1); X-ray crystallography (2)

Domain & Motif Annotations

Domain (CC)

Calcium-binding is structural and does not influence the alkaline phosphatase activity (PubMed:25775211). At very high concentrations, calcium can however substitute for zinc at zinc-binding sites, leading to strongly reduced enzyme activity (PubMed:25775211).

Protein Families

Alkaline phosphatase family

Sequence Similarities

Belongs to the alkaline phosphatase family.

Clinical Relevance5

Disease Involvement (2)

Cancer-related genesDisease variant

Related Diseases (5)

Colon cancer Genetic prion disease Mineral absorption/transport disorder Parasitic infection Pseudoxanthoma elasticum

Biomarker

Withdrawn from market; Approved; Phase 1; Phase 2

Drugs (54)

(+/-)-ERIODICTYOL MYRICETIN DANUGLIPRON CHEMBL:CHEMBL1256783 HAEMATOXYLIN CERTOLIZUMAB PEGOL SULFURETIN CHEMBL:CHEMBL56393 LSN3318839 CHEMBL:CHEMBL2094652 GLUCAGON (RDNA)GLUCAGON-LIKE PEPTIDE 1-(7-36) AMIDE INFLIXIMAB-DYYB SEMAGLUTIDE T-0632 PYROGALLOL RED ETANERCEPT-SZZS ISOQUERCETIN CHEMBL:CHEMBL81782 TERPYRIDINE AVEXITIDE EXENDIN-3 LIXISENATIDE EXENATIDE WB4-24 ADALIMUMAB-ADBM CHEMBL:CHEMBL600956 [125I]GLP-1-(7-36)-AMIDE EPICATECHIN CHEMBL:CHEMBL505807 LEVAMISOLE CHEMBL:CHEMBL598663 TIRZEPATIDE LIRAGLUTIDE CHEMBL:CHEMBL1408276 DIHYDRODAPHNODORIN B TRETINOIN GLUCAGON-LIKE PEPTIDE 1-(7-37)BETP [125I]EXENDIN-(9-39)THUNBERGINOL B CHEMBL:CHEMBL1731432 ASFOTASE ALFA CHEMBL:CHEMBL234338 CHEMBL:CHEMBL260311 CHEMBL:CHEMBL146710 CEPHALOCHROMIN PEPTIDE 5 [PMID: 28562585]NULL [125I]GLP-1-(7-37) (HUMAN)ORFORGLIPRON TYRPHOSTIN 23 CHEMBL:CHEMBL585591 CHEMBL:CHEMBL586135

Antibody (3)

HPA007105 HPA008765 CAB020829

Supporting Publications13

PMID	Title	Abstract
38716512	Assessment of urine sample collection and processing variables for extracellular vesicle-based proteomics.	No abstract available
40189497	Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.	No abstract available
40985879	TurboID-Mediated Profiling of Glioblastoma-Derived Extracellular Vesicle Cargo Proteins.	No abstract available

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