Protein detail

PPBT

Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP) (TNS-ALP) (TNSALP) (EC 3.1.3.1) (Alkaline phosphatase liver/bone/kidney isozyme) (Phosphoamidase) (Phosphocreatine phosphatase) (EC 3.9.1.1)

Entry name
PPBT
UniProt ID
P05186
EVMP confidence score
0.47
Supporting publications (n)
14
Transmembrane count
Protein classification
Cancer-related genesDisease related genesEnzymesHuman disease related genesMetabolic proteinsPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information11
Protein Names
Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP) (TNS-ALP) (TNSALP) (EC 3.1.3.1) (Alkaline phosphatase liver/bone/kidney isozyme) (Phosphoamidase) (Phosphocreatine phosphatase) (EC 3.9.1.1)
Protein Class (9)
Cancer-related genesDisease related genesEnzymesHuman disease related genesMetabolic proteinsPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins
Protein Function (7)
  • Human disease related genes:Congenital disorders of metabolism:Congenital disorders of cofactor/vitamin metabolism
  • Predicted intracellular proteins
  • Potential drug targets
  • Enzymes
  • Cancer-related genes:Candidate cancer biomarkers
  • ENZYME proteins:Hydrolases
  • Disease related genes
Ensembl
ENSG00000162551
Entrez Gene Symbol
ALPL
Gene Synonym (4)
HOPSTNALPTNAPTNSALP
Gene Description
Alkaline phosphatase, biomineralization associated
Chromosome
1
Position
21509397-21578410
Supporting publications (n)
14
EVMP confidence score
0.47
Fluorescence & Localization2
Tissue SpecifictestisCell SpecificEarly primary spermatocytes
Function & Pathway7
Protein Function (7)
  • Human disease related genes:Congenital disorders of metabolism:Congenital disorders of cofactor/vitamin metabolism
  • Predicted intracellular proteins
  • Potential drug targets
  • Enzymes
  • Cancer-related genes:Candidate cancer biomarkers
  • ENZYME proteins:Hydrolases
  • Disease related genes
Cellular Component (8)
Molecular Function (9)
Biological Process (3)
KEGG (4)
Reactome (2)
Mediation Categories (3)
Clinical-translation mediationImmune mediationMetabolism mediation
Relations & Evidence25

Ligand-Receptor Signaling (19)

19 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
ecmecmMatrixDBYesNoNoNoYes
ecmecmCellCellInteractionsYesNoNoNoYes
ecmecmOmniPathYesNoNoNoYes
extracellularextracellularOmniPathNoNoNoNoYes
intracellularintracellularLOCATENoNoNoNoYes
intracellularintracellularComPPINoNoNoNoYes
intracellularintracellularGO_IntercellNoNoNoNoYes
intracellularintracellularUniProt_locationNoNoNoNoYes
intracellularintracellularOmniPathNoNoNoNoYes
transmembranetransmembraneRamilowski_locationNoNoNoNoYes
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Regulatory Interaction Network (2)

2 records.

Source Protein SymbolSource UniProt IDTarget Protein SymbolTarget UniProt IDIs DirectedIs StimulationIs InhibitionDatabaseReferences
PPBTP05186TAUP10636YesNoYesSIGNORSIGNOR:24646911
PPBTP05186OSTPP10451YesNoYesSIGNORSIGNOR:23427088

Protein Complex Composition (3)

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyWestern blottingFlow cytometryMORPH3322181803370951032089743
Sequence, Structure & Domains12

Sequences

Length
524
Mass
57,305
Sequence
MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVLF
Alternative Products
Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=P05186-1; Sequence=Displayed; Name=2; IsoId=P05186-2; Sequence=VSP_042711; Name=3; IsoId=P05186-3; Sequence=VSP_044228
Alternative Sequence
1..99; MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSK -> MPWSFRSSTPTWLRMSSCSWEM (in isoform 2); 1..55; Missing (in isoform 3)

3D Structural Models

Turn
88..91; 176..178; 189..191; 303..305; 404..406
Helix
21..24; 26..42; 65..77; 111..119; 139..141; 150..156; 172..175; 194..198; 204..210; 252..257; 272..277; 280..282; 298..300; 309..320; 335..342; 345..365; 431..433; 468..470; 477..479; 480..487; 495..497
Beta Strand
52..59; 93..99; 103..107; 128..130; 160..168; 217..221; 224..228; 240..243; 246..248; 284..289; 291..294; 327..333; 370..378; 383..385; 411..418; 445..448; 459..465; 473..476
3D Structure
Electron microscopy (1); X-ray crystallography (2)

Domain & Motif Annotations

Domain (CC)
Calcium-binding is structural and does not influence the alkaline phosphatase activity (PubMed:25775211). At very high concentrations, calcium can however substitute for zinc at zinc-binding sites, leading to strongly reduced enzyme activity (PubMed:25775211).
Protein Families
Alkaline phosphatase family
Sequence Similarities
Belongs to the alkaline phosphatase family.
Clinical Relevance5
Disease Involvement (2)
Cancer-related genesDisease variant
Related Diseases (5)
Colon cancerGenetic prion diseaseMineral absorption/transport disorderParasitic infectionPseudoxanthoma elasticum
Biomarker
Withdrawn from market; Approved; Phase 1; Phase 2
Drugs (54)
(+/-)-ERIODICTYOLMYRICETINDANUGLIPRONCHEMBL:CHEMBL1256783HAEMATOXYLINCERTOLIZUMAB PEGOLSULFURETINCHEMBL:CHEMBL56393LSN3318839CHEMBL:CHEMBL2094652GLUCAGON (RDNA)GLUCAGON-LIKE PEPTIDE 1-(7-36) AMIDEINFLIXIMAB-DYYBSEMAGLUTIDET-0632PYROGALLOL REDETANERCEPT-SZZSISOQUERCETINCHEMBL:CHEMBL81782TERPYRIDINEAVEXITIDEEXENDIN-3LIXISENATIDEEXENATIDEWB4-24ADALIMUMAB-ADBMCHEMBL:CHEMBL600956[125I]GLP-1-(7-36)-AMIDEEPICATECHINCHEMBL:CHEMBL505807LEVAMISOLECHEMBL:CHEMBL598663TIRZEPATIDELIRAGLUTIDECHEMBL:CHEMBL1408276DIHYDRODAPHNODORIN BTRETINOINGLUCAGON-LIKE PEPTIDE 1-(7-37)BETP[125I]EXENDIN-(9-39)THUNBERGINOL BCHEMBL:CHEMBL1731432ASFOTASE ALFACHEMBL:CHEMBL234338CHEMBL:CHEMBL260311CHEMBL:CHEMBL146710CEPHALOCHROMINPEPTIDE 5 [PMID: 28562585]NULL[125I]GLP-1-(7-37) (HUMAN)ORFORGLIPRONTYRPHOSTIN 23CHEMBL:CHEMBL585591CHEMBL:CHEMBL586135
Antibody (3)
HPA007105HPA008765CAB020829
Supporting Publications13
PMIDTitleAbstract
26826536[Cardiovascular risk study in patients with renin-angiotensin system blockade by means of the proteone of circulating extracellular vesicles].No abstract available
27821849Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation.No abstract available
34064677Ubiquinone Metabolism and Transcription HIF-1 Targets Pathway Are Toxicity Signature Pathways Present in Extracellular Vesicles of Paraquat-Exposed Human Brain Microvascular Endothelial Cells.No abstract available
35119778Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis.No abstract available
36064647Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells.No abstract available
36146834Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro.No abstract available
37322475Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration.No abstract available
37926756Extracellular vesicles from non-neuroendocrine SCLC cells promote adhesion and survival of neuroendocrine SCLC cells.No abstract available
38113368In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.No abstract available
38207106Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.No abstract available
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