Protein detail

PPBT

Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP) (TNS-ALP) (TNSALP) (EC 3.1.3.1) (Alkaline phosphatase liver/bone/kidney isozyme) (Phosphoamidase) (Phosphocreatine phosphatase) (EC 3.9.1.1)

Entry name PPBT	UniProt ID P05186	EVMP score 0.47
Frequency 14	Transmembrane count	Protein classification Cancer-related genesDisease related genesEnzymesHuman disease related genesMetabolic proteinsPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP) (TNS-ALP) (TNSALP) (EC 3.1.3.1) (Alkaline phosphatase liver/bone/kidney isozyme) (Phosphoamidase) (Phosphocreatine phosphatase) (EC 3.9.1.1)

Protein Class

Cancer-related genesDisease related genesEnzymesHuman disease related genesMetabolic proteinsPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

Protein Function

Human disease related genes:Congenital disorders of metabolism:Congenital disorders of cofactor/vitamin metabolism
Predicted intracellular proteins
Potential drug targets
Enzymes
Cancer-related genes:Candidate cancer biomarkers
ENZYME proteins:Hydrolases
Disease related genes

Ensembl

ENSG00000162551

Entrez Gene Symbol

ALPL

Gene Synonym

HOPSTNALPTNAPTNSALP

Gene Description

Alkaline phosphatase, biomineralization associated

Chromosome

Position

21509397-21578410

Frequency

EVMP Score

0.47

Fluorescence & Localization

Tissue SpecifictestisCell SpecificEarly primary spermatocytes

Function & Pathway

Protein Function

Human disease related genes:Congenital disorders of metabolism:Congenital disorders of cofactor/vitamin metabolism
Predicted intracellular proteins
Potential drug targets
Enzymes
Cancer-related genes:Candidate cancer biomarkers
ENZYME proteins:Hydrolases
Disease related genes

Cellular Component

Molecular Function

Biological Process

KEGG

Reactome

Mediation Categories

Clinical-translation mediationImmune mediationMetabolism mediation

Relations & Evidence

Enzyme-Mediated Modification

0 records.

Ligand-Receptor Signaling

19 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	MatrixDB	Yes	No	No	No	Yes
ecm	ecm	CellCellInteractions	Yes	No	No	No	Yes
ecm	ecm	OmniPath	Yes	No	No	No	Yes
extracellular	extracellular	OmniPath	No	No	No	No	Yes
intracellular	intracellular	LOCATE	No	No	No	No	Yes
intracellular	intracellular	ComPPI	No	No	No	No	Yes
intracellular	intracellular	GO_Intercell	No	No	No	No	Yes
intracellular	intracellular	UniProt_location	No	No	No	No	Yes
intracellular	intracellular	OmniPath	No	No	No	No	Yes
transmembrane	transmembrane	Ramilowski_location	No	No	No	No	Yes

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Regulatory Interaction Network

2 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
PPBT	P05186	TAU	P10636	Yes	No	Yes	SIGNOR	SIGNOR:24646911
PPBT	P05186	OSTP	P10451	Yes	No	Yes	SIGNOR	SIGNOR:23427088

Protein Complex Composition

3 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ALPL EIF3A EIF3B EIF3F EIF3H EIF3I EIF3J EIF4A1 EIF4A2 EIF4E EIF4G1 EIF4G2 EIF4G3 PABPC1 SUMO1	O00303 O15372 O43432 O75822 P05186 P06730 P11940 P55884 P60842 P63165 P78344 Q04637 Q13347 Q14152 Q14240	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7035
ALPL	P05186	8	PDB	PDB:7yiwPDB:7yixPDB:7yiv
ALPL BIRC3 BIRC6 BIRC7 CASP9 CDC23	P05186 P55211 Q13489 Q96CA5 Q9NR09 Q9UJX2	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9558

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Western blottingFlow cytometryMORPH	3	32218180 33709510 32089743

Sequence, Structure & Domains

Sequences

Length

524

Mass

57,305

Sequence

MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVLF

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=P05186-1; Sequence=Displayed; Name=2; IsoId=P05186-2; Sequence=VSP_042711; Name=3; IsoId=P05186-3; Sequence=VSP_044228

Alternative Sequence

1..99; MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSK -> MPWSFRSSTPTWLRMSSCSWEM (in isoform 2); 1..55; Missing (in isoform 3)

3D Structural Models

Turn

88..91; 176..178; 189..191; 303..305; 404..406

Helix

21..24; 26..42; 65..77; 111..119; 139..141; 150..156; 172..175; 194..198; 204..210; 252..257; 272..277; 280..282; 298..300; 309..320; 335..342; 345..365; 431..433; 468..470; 477..479; 480..487; 495..497

Beta Strand

52..59; 93..99; 103..107; 128..130; 160..168; 217..221; 224..228; 240..243; 246..248; 284..289; 291..294; 327..333; 370..378; 383..385; 411..418; 445..448; 459..465; 473..476

3D Structure

Electron microscopy (1); X-ray crystallography (2)

Domain & Motif Annotations

Domain (CC)

Calcium-binding is structural and does not influence the alkaline phosphatase activity (PubMed:25775211). At very high concentrations, calcium can however substitute for zinc at zinc-binding sites, leading to strongly reduced enzyme activity (PubMed:25775211).

Protein Families

Alkaline phosphatase family

Sequence Similarities

Belongs to the alkaline phosphatase family.

Clinical Relevance

Disease Involvement

Cancer-related genesDisease variant

Related Diseases

Colon cancer Genetic prion disease Mineral absorption/transport disorder Parasitic infection Pseudoxanthoma elasticum

Biomarker

Withdrawn from market; Approved; Phase 1; Phase 2

Antibody

HPA007105 HPA008765 CAB020829