Protein detail

ACM4

Muscarinic acetylcholine receptor M4

Entry name ACM4	UniProt ID P08173	EVMP confidence score 0.38
Supporting publications (n) 4	Transmembrane count 7	Protein classification FDA approved drug targetsG-protein coupled receptorsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information12

Protein Names

Muscarinic acetylcholine receptor M4

Protein Class (3)

FDA approved drug targetsG-protein coupled receptorsPredicted membrane proteins

Protein Function (2)

FDA approved drug targets:Small molecule drugs
G-protein coupled receptors:GPCRs excl olfactory receptors

Transmembrane

32..54; Helical; Name=1; 69..89; Helical; Name=2; 107..128; Helical; Name=3; 149..171; Helical; Name=4; 194..216; Helical; Name=5; 402..422; Helical; Name=6; 437..456; Helical; Name=7

Transmembrane Count

Ensembl

ENSG00000180720

Entrez Gene Symbol

CHRM4

Gene Description

Cholinergic receptor muscarinic 4

Chromosome

Position

46383789-46391776

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization3

Tissue Specificbone marrowCell SpecificTuft cellsSingle-Nuclei Brain Specificcentral nervous system macrophage

Function & Pathway7

Protein Function (2)

FDA approved drug targets:Small molecule drugs
G-protein coupled receptors:GPCRs excl olfactory receptors

Cellular Component (4)

Molecular Function

GO:0016907 G protein-coupled acetylcholine receptor activity

Biological Process (3)

KEGG (4)

Reactome (6)

Mediation Categories (2)

Clinical-translation mediationReceptor-signaling mediation

Relations & Evidence45

Ligand-Receptor Signaling (40)

40 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
gpcr	receptor	Surfaceome	No	Yes	No	Yes	No
class_a_rhodopsin	receptor	GPCRdb	No	Yes	No	Yes	No
class_a_rhodopsin_aminergic	receptor	GPCRdb	No	Yes	No	Yes	No
class_a_rhodopsin_aminergic_acetylcholine_muscarinic	receptor	GPCRdb	No	Yes	No	Yes	No
receptor	receptor	OmniPath	No	Yes	No	Yes	No
extracellular	extracellular	HPMR	No	No	No	Yes	No
extracellular	extracellular	OmniPath	No	No	No	Yes	No
intracellular	intracellular	LOCATE	No	No	No	Yes	No
intracellular	intracellular	OmniPath	No	No	No	Yes	No
transmembrane	transmembrane	UniProt_location	No	No	No	Yes	No

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Regulatory Interaction Network (3)

3 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
ACM4	P08173	GNAI3	P08754	Yes	Yes	No	WangCui2007SIGNORCA1	CA1:7916637 CA1:10347206 SIGNOR:31160049
ACM4	P08173	GNAO	P09471	Yes	Yes	No	HINTSIGNOR	HINT:36450989 SIGNOR:31160049
ACM4	P08173	GNAI1	P63096	Yes	Yes	No	HINTSIGNOR	HINT:35606397 SIGNOR:31160049

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	CHRM4	P08173	2	PDB	PDB:5dsg

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
	Mass spectrometry	0

Sequence, Structure & Domains11

Sequences

Length

479

Mass

53,049

Sequence

MANFTPVNGSSGNQSVRLVTSSSHNRYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKQSVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVADKDTSNESSSGSATQNTKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR

3D Structural Models

Turn

137..142; 454..456

Helix

34..59; 61..63; 67..83; 85..95; 103..135; 146..174; 188..191; 193..203; 205..222; 226..229; 232..237; 393..425; 432..447; 449..453; 458..462

Beta Strand

175..177; 182..184; 223..225; 427..430

3D Structure

Electron microscopy (17); X-ray crystallography (2)

Domain & Motif Annotations

Compositional Bias

275..286; Pro residues; 294..304; Polar residues

Region

271..333; Disordered

Protein Families (3)

G-protein coupled receptor 1 family
Muscarinic acetylcholine receptor subfamily
CHRM4 sub-subfamily

Sequence Similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.

Clinical Relevance6

Disease Involvement

FDA approved drug targets

Related Diseases (6)

Asthma Attention deficit hyperactivity disorder Eye anterior segment structural developmental anomaly Glaucoma Nausea/vomiting Respiratory system disease

Biomarker

Approved; Investigative

Drug Targets

FDA approved drug targets

Antibody (2)

CAB022444 HPA072083

Supporting Publications4

PMID	Title	Abstract
32854315	Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study.	Recent studies have highlighted the importance of Aβ and tau-containing extracellular vesicles (EVs) in AD.
36064647	Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells.	No abstract available
38113368	In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.	No abstract available
41068253	Identification of plasma extracellular vesicle protein biomarkers in diabetic retinopathy progression.	No abstract available