Protein detail

HCK

Tyrosine-protein kinase HCK (EC 2.7.10.2) (Hematopoietic cell kinase) (Hemopoietic cell kinase) (p59-HCK/p60-HCK) (p59Hck) (p61Hck)

Entry name HCK	UniProt ID P08631	EVMP confidence score 0.38
Supporting publications (n) 4	Transmembrane count	Protein classification Disease related genesEnzymesPotential drug targetsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Tyrosine-protein kinase HCK (EC 2.7.10.2) (Hematopoietic cell kinase) (Hemopoietic cell kinase) (p59-HCK/p60-HCK) (p59Hck) (p61Hck)

Protein Class (4)

Disease related genesEnzymesPotential drug targetsPredicted intracellular proteins

Protein Function (6)

Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Kinases:Tyr protein kinases
Disease related genes

Ensembl

ENSG00000101336

Entrez Gene Symbol

HCK

Gene Synonym

JTK9

Gene Description

HCK proto-oncogene, Src family tyrosine kinase

Chromosome

Position

32052197-32101856

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization3

Tissue SpecificbrainCell SpecificBrain excitatory neurons

Function & Pathway7

Protein Function (6)

Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Kinases:Tyr protein kinases
Disease related genes

Cellular Component (12)

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Molecular Function (7)

Biological Process (3)

KEGG (3)

Reactome (22)

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Mediation Categories (4)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediation

Relations & Evidence46

Enzyme-Mediated Modification (9)

9 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
HCK	CHEK1	O14757	Y	411	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
HCK	CSK	P41240	Y	411	phosphorylation	NCI-PID_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper	ProtMapper:16537894 ProtMapper:11489946 ProtMapper:14967142
HCK	CSK	P41240	Y	522	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEA	KEA:10644735 KEA:11976726
HCK	AXL	P30530	Y	411	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
HCK	GNAI2	P04899	Y	411	phosphorylation	NCI-PID_ProtMapperProtMapper	ProtMapper:11160627
HCK	PTK2B	Q14289	Y	411	phosphorylation	NCI-PID_ProtMapperProtMapper
HCK	PTPN13	Q12923	Y	411	phosphorylation	NCI-PID_ProtMapperProtMapper	ProtMapper:11983165
HCK	INSR	P06213	Y	410	phosphorylation	KEA	KEA:17570479
HCK	LYN	P07948	Y	410	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (7)

7 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network (28)

28 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
HCK	P08631	ABL1	P00519	Yes	Yes	No	DOMINOSPIKEWangSparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPNCI-PID_ProtMapperHPRD_MIMPPhosphoSite_norefiPTMnetProtMapperHPRDKEAKinexus_KEASPIKE_LCPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:24708674 SPIKE:18775435 ProtMapper:16787943 HPRD:10849448 SPIKE_LC:20841568 DOMINO:10849448 ProtMapper:11847100 PhosphoSite:26473374 ProtMapper:16076903 SPIKE:20841568 SPIKE_LC:18775435 HPRD:9407116 ProtMapper:18775435 PhosphoSite:28087699 SPIKE_LC:10849448 KEA:16912036 KEA:12522270
HCK	P08631	CBL	P22681	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEALit-BM-17PhosphoNetworksHPRDWangHPRD-phosphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEANetPathSPIKE_LCSPIKE	ProtMapper:10092522 ProtMapper:10799548 KEA:9525940 HPRD-phos:10092522 KEA:10092522 HPRD-phos:11239464 HPRD:11994282 KEA:12604776 KEA:10799548 Lit-BM-17:8995358 SPIKE_LC:8995358 KEA:11239464 ProtMapper:9890970 KEA:11994282 ProtMapper:11239464 ProtMapper:11994282 SPIKE:8995358 KEA:10934191 SIGNOR:10092522 Lit-BM-17:10092522 HPRD:10092522 Lit-BM-17:11896602 NetPath:8995358 HPRD-phos:9890970 HPRD:11239464 HPRD-phos:10799548 HPRD-phos:11994282 HPRD:9890970 KEA:9890970 HPRD:10799548 iPTMnet:9890970
HCK	P08631	SHC1	P29353	Yes	Yes	No	WangSignaLink3TCRcuration_SignaLink3	SignaLink3:23109003 SignaLink3:10329689
HCK	P08631	PAXI	P49023	Yes	Yes	No	Sparser_ProtMapperProtMapperSignaLink3WangTCRcuration_SignaLink3	SignaLink3:23109003 SignaLink3:9636140 ProtMapper:16809022
HCK	P08631	FAK1	Q05397	Yes	Yes	No	NCI-PID_ProtMapperProtMapperSignaLink3WangLit-BM-17TCRcuration_SignaLink3	ProtMapper:12590707 SignaLink3:23109003 SignaLink3:12495636 SignaLink3:9148935 SignaLink3:7529876 ProtMapper:10753828 ProtMapper:11369622 Lit-BM-17:25814554 SignaLink3:10593973 ProtMapper:15728464
HCK	P08631	FAK2	Q14289	Yes	Yes	No	KEASignaLink3WangNetworKIN_KEATCRcuration_SignaLink3	SignaLink3:23109003 SignaLink3:12495636 SignaLink3:7529876 KEA:17570479 SignaLink3:10593973
FAK2	Q14289	HCK	P08631	Yes	Yes	No	NCI-PID_ProtMapperProtMapperSignaLink3WangTCRcuration_SignaLink3	SignaLink3:23109003 SignaLink3:9148935
HCK	P08631	P85A	P27986	Yes	Yes	No	WangPhosphoPointHPRDSignaLink3SPIKE_LCTCRcuration_SignaLink3	HPRD:12029088 SignaLink3:10488096 SignaLink3:22880054 SignaLink3:11526404 SignaLink3:23109003 SPIKE_LC:17145710 SignaLink3:21780115 SignaLink3:15032589
HCK	P08631		Q96EV4	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:10488096 SignaLink3:22880054 SignaLink3:11526404 SignaLink3:23109003 SignaLink3:21780115 SignaLink3:15032589
GNAI1	P63096	HCK	P08631	Yes	Yes	No	TCRcuration_SignaLink3SIGNORSignaLink3	SignaLink3:10753828 SignaLink3:23109003 SignaLink3:11007482 SIGNOR:11007482 SignaLink3:9722546

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Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	HCK	P08631	2	PDB	PDB:3vrzPDB:3vryPDB:2c0iPDB:2c0tPDB:3vs6PDB:3vs5PDB:5zj6PDB:1bu1PDB:3vs0PDB:3vs7PDB:2hckPDB:3vs3PDB:3vs1PDB:3vs4PDB:2c0oPDB:1ad5PDB:4luePDB:4ludPDB:3vs2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryR Sequencing	1	30865381

Sequence, Structure & Domains15

Sequences

Length

526

Mass

59,600

Sequence

MGGRSSCEDPGCPRDEERAPRMGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP

Alternative Products

Event=Alternative splicing, Alternative initiation; Named isoforms=4; Name=1; Synonyms=p60-HCK, p61Hck; IsoId=P08631-1; Sequence=Displayed; Name=2; Synonyms=p59-HCK, p59Hck; IsoId=P08631-2; Sequence=VSP_018858; Name=3; IsoId=P08631-3; Sequence=VSP_018858, VSP_041926; Name=4; IsoId=P08631-4; Sequence=VSP_041926

Alternative Sequence

1..21; Missing (in isoform 2 and isoform 3); 76; Missing (in isoform 3 and isoform 4)

3D Structural Models

Turn

189..191; 252..255; 282..284; 452..454; 513..518

Helix

130..132; 136..138; 139..141; 151..159; 219..228; 259..261; 299..309; 341..345; 350..352; 355..374; 384..386; 402..405; 410..413; 421..423; 426..431; 436..451; 463..471; 484..493; 498..500; 504..512

Beta Strand

82..85; 93..96; 99..102; 104..111; 114..122; 127..129; 133..135; 145..148; 168..172; 174..176; 180..188; 192..202; 204..206; 208..211; 215..218; 233..235; 262..270; 272..281; 285..292; 294..297; 320..324; 326..328; 330..333; 387..389; 395..397; 416..419; 457..460; 480..482; 520..522

3D Structure

NMR spectroscopy (5); X-ray crystallography (35)

Domain & Motif Annotations

Compositional Bias

11..20; Basic and acidic residues

Domain (CC)

The SH3 domain mediates binding to HIV-1 Nef.

Domain (FT)

78..138; SH3; 144..241; SH2; 262..515; Protein kinase

Region

1..20; Disordered

Protein Families (3)

Protein kinase superfamily
Tyr protein kinase family
SRC subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Clinical Relevance8

Disease Involvement

Proto-oncogene

Related Diseases

Indeterminate colitis

Biomarker

Discontinued in Phase 2

Antibody (3)

CAB005195 HPA035534 HPA063768

Interaction Protein (5)

ENSG00000096384ENSG00000121774ENSG00000143537ENSG00000146648ENSG00000155849

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications4

PMID	Title	Abstract
38037300	Proteomic profiling of paired human liver homogenate and tissue derived extracellular vesicles.	No abstract available
39702169	iTRAQ proteomic analysis of exosomes derived from synovial fluid reveals disease patterns and potential biomarkers of Osteoarthritis.	No abstract available
40596376	Proteomic profiling of plasma extracellular vesicles identifies signatures of innate immunity, coagulation, and endothelial activation in septic patients.	No abstract available
40689422	Defining the Ovarian Cancer Precancerous Landscape through Modeling Fallopian Tube Epithelium Reprogramming Driven by Extracellular Vesicles.	No abstract available