EV MemProt Atlas

Basic Information11

Protein Names

Tyrosine-protein kinase HCK (EC 2.7.10.2) (Hematopoietic cell kinase) (Hemopoietic cell kinase) (p59-HCK/p60-HCK) (p59Hck) (p61Hck)

Protein Class (4)

Disease related genesEnzymesPotential drug targetsPredicted intracellular proteins

Protein Function (6)

Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Kinases:Tyr protein kinases
Disease related genes

Ensembl

ENSG00000101336

Entrez Gene Symbol

HCK

Gene Synonym

JTK9

Gene Description

HCK proto-oncogene, Src family tyrosine kinase

Chromosome

20

Position

32052197-32101856

Supporting publications (n)

4

EVMP confidence score

0.38

Fluorescence & Localization3

Tissue SpecificbrainCell SpecificBrain excitatory neurons

Function & Pathway7

Protein Function (6)

Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Kinases:Tyr protein kinases
Disease related genes

Cellular Component (12)

Page 1 of 2

Molecular Function (7)

Biological Process (3)

KEGG (3)

Reactome (22)

Page 1 of 3

Mediation Categories (4)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediation

Relations & Evidence46

Enzyme-Mediated Modification (9)

9 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
HCK	CHEK1	O14757	Y	411	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
HCK	CSK	P41240	Y	411	phosphorylation	NCI-PID_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper	ProtMapper:16537894 ProtMapper:11489946 ProtMapper:14967142
HCK	CSK	P41240	Y	522	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEA	KEA:10644735 KEA:11976726
HCK	AXL	P30530	Y	411	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
HCK	GNAI2	P04899	Y	411	phosphorylation	NCI-PID_ProtMapperProtMapper	ProtMapper:11160627
HCK	PTK2B	Q14289	Y	411	phosphorylation	NCI-PID_ProtMapperProtMapper
HCK	PTPN13	Q12923	Y	411	phosphorylation	NCI-PID_ProtMapperProtMapper	ProtMapper:11983165
HCK	INSR	P06213	Y	410	phosphorylation	KEA	KEA:17570479
HCK	LYN	P07948	Y	410	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (7)

7 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network (28)

28 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
GNAI2	P04899	HCK	P08631	Yes	Yes	No	NCI-PID_ProtMapperProtMapperSignaLink3WangTCRcuration_SignaLink3	SignaLink3:10753828 ProtMapper:11160627 SignaLink3:23109003 SignaLink3:11007482 SignaLink3:9722546
GNAI3	P08754	HCK	P08631	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:23109003 SignaLink3:9722546 SignaLink3:10753828 SignaLink3:11007482
HCK	P08631	ELMO1	Q92556	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperdbPTMPhosphoSite_KEASignaLink3HPRDWangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointiPTMnetKEAHPRD_KEASIGNOR_ProtMapperREACH_ProtMapperPhosphoSiteTCRcuration_SignaLink3Sparser_ProtMapperSPIKE_LC	HPRD:12029088 ProtMapper:26205662 PhosphoSite:24928514 SignaLink3:23109003 dbPTM:15952790 SPIKE_LC:17145710 SIGNOR:15952790 SignaLink3:15952790 KEA:15952790 ProtMapper:15952790
HCK	P08631	PLCG1	P19174	Yes	Yes	No	WangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDCui2007PhosphoSite_KEAKEACA1HPRD_KEASIGNOR_ProtMapperHPRD-phos	HPRD:15144186 ProtMapper:7682059 KEA:16126727 KEA:16153436 ProtMapper:18707149 KEA:8885868 HPRD-phos:15144186 ProtMapper:8885868 HPRD:1689310 ProtMapper:17016520 KEA:17081983 HPRD-phos:20068231 HPRD-phos:7682059 KEA:12601080 CA1:7682059 KEA:7682059 ProtMapper:17389395 HPRD-phos:1689310 KEA:15144186 KEA:8657103 KEA:1689310 KEA:15592455 HPRD:8885868 ProtMapper:1689310 HPRD-phos:18707149 ProtMapper:8657103 HPRD:7682059 HPRD:10586033 ProtMapper:15144186 HPRD-phos:17389395 HPRD-phos:8885868 KEA:1708307 HPRD:8657103 ProtMapper:16497976 SIGNOR:7682059 HPRD-phos:8657103 ProtMapper:20068231 HPRD-phos:16497976 KEA:16038803 HPRD-phos:17016520
HCK	P08631	CSF3R	Q99062	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapper	ProtMapper:9790917 KEA:9790917 HPRD:9790917 KEA:8547634 SIGNOR:9790917
HCK	P08631	RPGF1	Q13905	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperdbPTMPhosphoSite_KEAphosphoELM_KEALit-BM-17PhosphoNetworksHPRDPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSiteHPRD-phos	SIGNOR:24396067 KEA:15320955 ProtMapper:14551197 KEA:10318861 HPRD:14551197 ProtMapper:10318861 PhosphoSite:14551197 HPRD-phos:12734187 ProtMapper:24396067 PhosphoSite:21876762 HPRD-phos:14551197 Lit-BM-17:14551197 PhosphoSite:15320955 dbPTM:14551197 PhosphoSite:10318861 PhosphoSite:30567575 PhosphoSite:18957052 KEA:14551197 PhosphoSite:28790195 PhosphoSite:32296045 PhosphoSite:20581864 KEA:12734187 phosphoELM:14551197 phosphoELM:15320955 ProtMapper:12734187 PhosphoSite:24396067 HPRD-phos:10318861 PhosphoSite:12734187 PhosphoSite:28273099 HPRD:12734187 HPRD:10318861
HCK	P08631	GLI1	P08151	Yes	Yes	No	SIGNOR	SIGNOR:26619401
HCK	P08631	STAT3	P40763	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPNCI-PID_ProtMapperHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperHPRD-phos	ProtMapper:11404481 HPRD-phos:12244095 HPRD:10918587 ProtMapper:10815799 KEA:8272872 HPRD-phos:12626508 KEA:12626508 KEA:9582023 ProtMapper:11350938 HPRD-phos:10918587 KEA:11940572 KEA:9083098 HPRD:14551213 HPRD:12244095 KEA:11850821 KEA:12244095 ProtMapper:17016520 KEA:9305919 ProtMapper:10918587 ProtMapper:11294897 KEA:10918587 HPRD-phos:11350938 ProtMapper:12244095 ProtMapper:17389395 HPRD:11940572 HPRD:12626508 HPRD:12576423 HPRD-phos:12576423 KEA:8626374 ProtMapper:14551213 KEA:15592455 ProtMapper:9484840 SIGNOR:12244095 KEA:11350938 HPRD:11350938 HPRD-phos:19901323 HPRD-phos:11294897 HPRD-phos:14551213 KEA:9566874 HPRD-phos:17389395 KEA:11294897 ProtMapper:16497976 KEA:14551213 ProtMapper:19901323 HPRD-phos:8626374 ProtMapper:9344858 HPRD-phos:16497976 HPRD:8626374 ProtMapper:11940572 HPRD:11294897 ProtMapper:12626508 ProtMapper:8626374 ProtMapper:12576423 HPRD-phos:11940572 KEA:11429593 HPRD-phos:17016520 KEA:12576423
HCK	P08631	ADA15	Q13444	Yes	Yes	No	SIGNORProtMapperdbPTMHINTPhosphoSite_KEAphosphoELM_KEADOMINOPhosphoNetworksHPRDIntActPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAphosphoELMSIGNOR_ProtMapperPhosphoSiteSPIKE_LC	dbPTM:11741929 IntAct:11741929 HINT:16374509 IntAct:25825872 HPRD:11741929 PhosphoSite:11741929 HINT:25825872 DOMINO:11741929 HPRD:16374509 KEA:11741929 phosphoELM:11741929 SIGNOR:11741929 ProtMapper:11741929 IntAct:16374509 SPIKE_LC:11741929 DOMINO:16374509 SPIKE_LC:16189514 HINT:11741929
HCK	P08631	TBK1	Q9UHD2	Yes	No	Yes	PhosphoSite_norefSIGNORProtMapperRLIMS-P_ProtMapperPhosphoSite_ProtMapper	SIGNOR:28618271 ProtMapper:28618271

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Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	HCK	P08631	2	PDB	PDB:3vrzPDB:3vryPDB:2c0iPDB:2c0tPDB:3vs6PDB:3vs5PDB:5zj6PDB:1bu1PDB:3vs0PDB:3vs7PDB:2hckPDB:3vs3PDB:3vs1PDB:3vs4PDB:2c0oPDB:1ad5PDB:4luePDB:4ludPDB:3vs2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryR Sequencing	1	30865381

Sequence, Structure & Domains15

Sequences

Length

526

Mass

59,600

Sequence

MGGRSSCEDPGCPRDEERAPRMGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP

Alternative Products

Event=Alternative splicing, Alternative initiation; Named isoforms=4; Name=1; Synonyms=p60-HCK, p61Hck; IsoId=P08631-1; Sequence=Displayed; Name=2; Synonyms=p59-HCK, p59Hck; IsoId=P08631-2; Sequence=VSP_018858; Name=3; IsoId=P08631-3; Sequence=VSP_018858, VSP_041926; Name=4; IsoId=P08631-4; Sequence=VSP_041926

Alternative Sequence

1..21; Missing (in isoform 2 and isoform 3); 76; Missing (in isoform 3 and isoform 4)

3D Structural Models

Turn

189..191; 252..255; 282..284; 452..454; 513..518

Helix

130..132; 136..138; 139..141; 151..159; 219..228; 259..261; 299..309; 341..345; 350..352; 355..374; 384..386; 402..405; 410..413; 421..423; 426..431; 436..451; 463..471; 484..493; 498..500; 504..512

Beta Strand

82..85; 93..96; 99..102; 104..111; 114..122; 127..129; 133..135; 145..148; 168..172; 174..176; 180..188; 192..202; 204..206; 208..211; 215..218; 233..235; 262..270; 272..281; 285..292; 294..297; 320..324; 326..328; 330..333; 387..389; 395..397; 416..419; 457..460; 480..482; 520..522

3D Structure

NMR spectroscopy (5); X-ray crystallography (35)

Domain & Motif Annotations

Compositional Bias

11..20; Basic and acidic residues

Domain (CC)

The SH3 domain mediates binding to HIV-1 Nef.

Domain (FT)

78..138; SH3; 144..241; SH2; 262..515; Protein kinase

Region

1..20; Disordered

Protein Families (3)

Protein kinase superfamily
Tyr protein kinase family
SRC subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Clinical Relevance8

Disease Involvement

Proto-oncogene

Related Diseases

Indeterminate colitis

Biomarker

Discontinued in Phase 2

Antibody (3)

CAB005195 HPA035534 HPA063768

Interaction Protein (5)

ENSG00000096384ENSG00000121774ENSG00000143537ENSG00000146648ENSG00000155849

Interaction Count

5

Interaction Dataset

intact_biogrid

Supporting Publications4

PMID	Title	Abstract
38037300	Proteomic profiling of paired human liver homogenate and tissue derived extracellular vesicles.	No abstract available
39702169	iTRAQ proteomic analysis of exosomes derived from synovial fluid reveals disease patterns and potential biomarkers of Osteoarthritis.	No abstract available
40596376	Proteomic profiling of plasma extracellular vesicles identifies signatures of innate immunity, coagulation, and endothelial activation in septic patients.	No abstract available
40689422	Defining the Ovarian Cancer Precancerous Landscape through Modeling Fallopian Tube Epithelium Reprogramming Driven by Extracellular Vesicles.	No abstract available

Entry name HCK	UniProt ID P08631	EVMP confidence score 0.38
Supporting publications (n) 4	Transmembrane count	Protein classification Disease related genesEnzymesPotential drug targetsPredicted intracellular proteins