Protein detail

HCK

Tyrosine-protein kinase HCK (EC 2.7.10.2) (Hematopoietic cell kinase) (Hemopoietic cell kinase) (p59-HCK/p60-HCK) (p59Hck) (p61Hck)

Entry name
HCK
UniProt ID
EVMP score
0.38
Frequency
4
Transmembrane count
Protein classification
Disease related genesEnzymesPotential drug targetsPredicted intracellular proteins
EVMP score: annotation confidence score.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information
Protein Names
Tyrosine-protein kinase HCK (EC 2.7.10.2) (Hematopoietic cell kinase) (Hemopoietic cell kinase) (p59-HCK/p60-HCK) (p59Hck) (p61Hck)
Protein Class
Disease related genesEnzymesPotential drug targetsPredicted intracellular proteins
Protein Function
  • Predicted intracellular proteins
  • ENZYME proteins:Transferases
  • Potential drug targets
  • Enzymes
  • Kinases:Tyr protein kinases
  • Disease related genes
Entrez Gene Symbol
Gene Synonym
JTK9
Gene Description
HCK proto-oncogene, Src family tyrosine kinase
Chromosome
20
Position
32052197-32101856
Frequency
4
EVMP Score
0.38
Fluorescence & Localization
HCK fluorescence
Tissue SpecificbrainCell SpecificBrain excitatory neurons
Function & Pathway
Protein Function
  • Predicted intracellular proteins
  • ENZYME proteins:Transferases
  • Potential drug targets
  • Enzymes
  • Kinases:Tyr protein kinases
  • Disease related genes
Mediation Categories
Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediation
Relations & Evidence

Enzyme-Mediated Modification

9 records.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
HCKCHEK1O14757Y411phosphorylationPhosphoSitePhosphoSite_ProtMapperProtMapper
HCKCSKP41240Y411phosphorylationNCI-PID_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapperProtMapper:16537894ProtMapper:11489946ProtMapper:14967142
HCKCSKP41240Y522phosphorylationphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEAKEA:10644735KEA:11976726
HCKAXLP30530Y411phosphorylationPhosphoSitePhosphoSite_ProtMapperProtMapper
HCKGNAI2P04899Y411phosphorylationNCI-PID_ProtMapperProtMapperProtMapper:11160627
HCKPTK2BQ14289Y411phosphorylationNCI-PID_ProtMapperProtMapper
HCKPTPN13Q12923Y411phosphorylationNCI-PID_ProtMapperProtMapperProtMapper:11983165
HCKINSRP06213Y410phosphorylationKEAKEA:17570479
HCKLYNP07948Y410phosphorylationKEAKEA:17570479

Ligand-Receptor Signaling

7 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
receptorreceptorOmniPathNoYesNoNoNo
intracellularintracellularLOCATENoNoNoNoNo
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo
receptorreceptorscConnectNoYesNoNoNo

Regulatory Interaction Network

28 records.

Source Protein SymbolSource UniProt IDTarget Protein SymbolTarget UniProt IDIs DirectedIs StimulationIs InhibitionDatabaseReferences
GNAS3O95467HCKP08631YesYesNoSIGNORSIGNOR:11007482
ALEXP84996HCKP08631YesYesNoSIGNORSIGNOR:11007482
GNAS2P63092HCKP08631YesYesNoSIGNORSIGNOR:11007482
GNAS1Q5JWF2HCKP08631YesYesNoSIGNORSIGNOR:11007482
HCKP08631PLCG2P16885YesYesNoWangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperHPRD-phosHPRD:15144186ProtMapper:7682059KEA:11606584KEA:11507089ProtMapper:12181444HPRD:12181444KEA:14656219HPRD-phos:7682059KEA:7682059HPRD-phos:12181444KEA:15144186HPRD-phos:19901323HPRD:7682059ProtMapper:15144186KEA:12181444ProtMapper:16497976ProtMapper:19901323SIGNOR:7682059HPRD-phos:16497976
HCKP08631WASPP42768YesYesNoHPRD_MIMPSIGNORProtMapperdbPTMHINTPhosphoSite_KEAphosphoELM_KEAPhosphoNetworksHPRDIntActphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperSparser_ProtMapperSPIKE_LCHPRD-phosHPRD:12029088HPRD:10068673KEA:12769847SPIKE_LC:17145710HPRD-phos:12235133HPRD-phos:18088087HPRD-phos:12769846ProtMapper:12235133dbPTM:15592455HPRD:12769846ProtMapper:14707117HINT:19234535dbPTM:18083107IntAct:19234535ProtMapper:10068673dbPTM:14707117ProtMapper:18088087ProtMapper:9742969KEA:12769846HPRD-phos:9742969phosphoELM:12235133KEA:9742969dbPTM:19690332KEA:18088087HINT:12029088HPRD:9742969ProtMapper:12769846KEA:15592455KEA:12235133KEA:10068673SIGNOR:12235133HPRD-phos:19901323dbPTM:18088087IntAct:12029088ProtMapper:16497976ProtMapper:19901323HPRD:12235133HPRD-phos:10068673HPRD-phos:16497976dbPTM:12235133
CHK1O14757HCKP08631YesNoNoPhosphoSitePhosphoSite_ProtMapperProtMapperPhosphoSite:19664994PhosphoSite:21447384PhosphoSite:20068231PhosphoSite:21609022PhosphoSite:23882028PhosphoSite:16946300PhosphoSite:22115753PhosphoSite:18088087PhosphoSite:20097925PhosphoSite:16464493PhosphoSite:25814448PhosphoSite:28784162PhosphoSite:9822689PhosphoSite:19658100PhosphoSite:23186163PhosphoSite:21437283PhosphoSite:27595981PhosphoSite:17016520PhosphoSite:22461510PhosphoSite:18691976PhosphoSite:21403953PhosphoSite:24667141PhosphoSite:19901323PhosphoSite:27251275PhosphoSite:19534553PhosphoSite:22617229PhosphoSite:20736484PhosphoSite:20090780PhosphoSite:23438512PhosphoSite:23836654PhosphoSite:10644735PhosphoSite:17360941PhosphoSite:21552520PhosphoSite:19369195PhosphoSite:23911959PhosphoSite:21376226PhosphoSite:22964225PhosphoSite:21253578PhosphoSite:34132782PhosphoSite:22366891PhosphoSite:23716303PhosphoSite:21394647PhosphoSite:24927040PhosphoSite:20541700PhosphoSite:16497976
HCKP08631P73O15350YesNoNoiPTMnetProtMapperRLIMS-P_ProtMapperdbPTMPhosphoSitePhosphoSite_ProtMapperdbPTM:17535448PhosphoSite:17535448ProtMapper:17535448
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Protein Complex Composition

1 record.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
HCKP086312PDBPDB:3vrzPDB:3vryPDB:2c0iPDB:2c0tPDB:3vs6PDB:3vs5PDB:5zj6PDB:1bu1PDB:3vs0PDB:3vs7PDB:2hckPDB:3vs3PDB:3vs1PDB:3vs4PDB:2c0oPDB:1ad5PDB:4luePDB:4ludPDB:3vs2

Isolation & Detection Technology

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationSize Exclusion ChromatographyMass spectrometryR Sequencing130865381
Sequence, Structure & Domains

Sequences

Length
526
Mass
59,600
Sequence
MGGRSSCEDPGCPRDEERAPRMGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP
Alternative Products
Event=Alternative splicing, Alternative initiation; Named isoforms=4; Name=1; Synonyms=p60-HCK, p61Hck; IsoId=P08631-1; Sequence=Displayed; Name=2; Synonyms=p59-HCK, p59Hck; IsoId=P08631-2; Sequence=VSP_018858; Name=3; IsoId=P08631-3; Sequence=VSP_018858, VSP_041926; Name=4; IsoId=P08631-4; Sequence=VSP_041926
Alternative Sequence
1..21; Missing (in isoform 2 and isoform 3); 76; Missing (in isoform 3 and isoform 4)

3D Structural Models

Turn
189..191; 252..255; 282..284; 452..454; 513..518
Helix
130..132; 136..138; 139..141; 151..159; 219..228; 259..261; 299..309; 341..345; 350..352; 355..374; 384..386; 402..405; 410..413; 421..423; 426..431; 436..451; 463..471; 484..493; 498..500; 504..512
Beta Strand
82..85; 93..96; 99..102; 104..111; 114..122; 127..129; 133..135; 145..148; 168..172; 174..176; 180..188; 192..202; 204..206; 208..211; 215..218; 233..235; 262..270; 272..281; 285..292; 294..297; 320..324; 326..328; 330..333; 387..389; 395..397; 416..419; 457..460; 480..482; 520..522
3D Structure
NMR spectroscopy (5); X-ray crystallography (35)

Domain & Motif Annotations

Compositional Bias
11..20; Basic and acidic residues
Domain (CC)
The SH3 domain mediates binding to HIV-1 Nef.
Domain (FT)
78..138; SH3; 144..241; SH2; 262..515; Protein kinase
Region
1..20; Disordered
Protein Families
  • Protein kinase superfamily
  • Tyr protein kinase family
  • SRC subfamily
Sequence Similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Clinical Relevance
Disease Involvement
Proto-oncogene
Related Diseases
Biomarker
Discontinued in Phase 2
Interaction Protein
ENSG00000096384ENSG00000121774ENSG00000143537ENSG00000146648ENSG00000155849
Interaction Count
5
Interaction Dataset
intact_biogrid