Protein detail

HCK

Tyrosine-protein kinase HCK (EC 2.7.10.2) (Hematopoietic cell kinase) (Hemopoietic cell kinase) (p59-HCK/p60-HCK) (p59Hck) (p61Hck)

Entry name HCK	UniProt ID P08631	EVMP confidence score 0.38
Supporting publications (n) 4	Transmembrane count	Protein classification Disease related genesEnzymesPotential drug targetsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Tyrosine-protein kinase HCK (EC 2.7.10.2) (Hematopoietic cell kinase) (Hemopoietic cell kinase) (p59-HCK/p60-HCK) (p59Hck) (p61Hck)

Protein Class (4)

Disease related genesEnzymesPotential drug targetsPredicted intracellular proteins

Protein Function (6)

Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Kinases:Tyr protein kinases
Disease related genes

Ensembl

ENSG00000101336

Entrez Gene Symbol

HCK

Gene Synonym

JTK9

Gene Description

HCK proto-oncogene, Src family tyrosine kinase

Chromosome

Position

32052197-32101856

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization3

Tissue SpecificbrainCell SpecificBrain excitatory neurons

Function & Pathway7

Protein Function (6)

Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Kinases:Tyr protein kinases
Disease related genes

Cellular Component (12)

Page 1 of 2

Molecular Function (7)

Biological Process (3)

KEGG (3)

Reactome (22)

Page 1 of 3

Mediation Categories (4)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediation

Relations & Evidence46

Enzyme-Mediated Modification (9)

9 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
HCK	CHEK1	O14757	Y	411	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
HCK	CSK	P41240	Y	411	phosphorylation	NCI-PID_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper	ProtMapper:16537894 ProtMapper:11489946 ProtMapper:14967142
HCK	CSK	P41240	Y	522	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEA	KEA:10644735 KEA:11976726
HCK	AXL	P30530	Y	411	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
HCK	GNAI2	P04899	Y	411	phosphorylation	NCI-PID_ProtMapperProtMapper	ProtMapper:11160627
HCK	PTK2B	Q14289	Y	411	phosphorylation	NCI-PID_ProtMapperProtMapper
HCK	PTPN13	Q12923	Y	411	phosphorylation	NCI-PID_ProtMapperProtMapper	ProtMapper:11983165
HCK	INSR	P06213	Y	410	phosphorylation	KEA	KEA:17570479
HCK	LYN	P07948	Y	410	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (7)

7 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network (28)

28 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
GNAS3	O95467	HCK	P08631	Yes	Yes	No	SIGNOR	SIGNOR:11007482
ALEX	P84996	HCK	P08631	Yes	Yes	No	SIGNOR	SIGNOR:11007482
GNAS2	P63092	HCK	P08631	Yes	Yes	No	SIGNOR	SIGNOR:11007482
GNAS1	Q5JWF2	HCK	P08631	Yes	Yes	No	SIGNOR	SIGNOR:11007482
HCK	P08631	PLCG2	P16885	Yes	Yes	No	WangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperHPRD-phos	HPRD:15144186 ProtMapper:7682059 KEA:11606584 KEA:11507089 ProtMapper:12181444 HPRD:12181444 KEA:14656219 HPRD-phos:7682059 KEA:7682059 HPRD-phos:12181444 KEA:15144186 HPRD-phos:19901323 HPRD:7682059 ProtMapper:15144186 KEA:12181444 ProtMapper:16497976 ProtMapper:19901323 SIGNOR:7682059 HPRD-phos:16497976
HCK	P08631	WASP	P42768	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperdbPTMHINTPhosphoSite_KEAphosphoELM_KEAPhosphoNetworksHPRDIntActphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperSparser_ProtMapperSPIKE_LCHPRD-phos	HPRD:12029088 HPRD:10068673 KEA:12769847 SPIKE_LC:17145710 HPRD-phos:12235133 HPRD-phos:18088087 HPRD-phos:12769846 ProtMapper:12235133 dbPTM:15592455 HPRD:12769846 ProtMapper:14707117 HINT:19234535 dbPTM:18083107 IntAct:19234535 ProtMapper:10068673 dbPTM:14707117 ProtMapper:18088087 ProtMapper:9742969 KEA:12769846 HPRD-phos:9742969 phosphoELM:12235133 KEA:9742969 dbPTM:19690332 KEA:18088087 HINT:12029088 HPRD:9742969 ProtMapper:12769846 KEA:15592455 KEA:12235133 KEA:10068673 SIGNOR:12235133 HPRD-phos:19901323 dbPTM:18088087 IntAct:12029088 ProtMapper:16497976 ProtMapper:19901323 HPRD:12235133 HPRD-phos:10068673 HPRD-phos:16497976 dbPTM:12235133
CHK1	O14757	HCK	P08631	Yes	No	No	PhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:19664994 PhosphoSite:21447384 PhosphoSite:20068231 PhosphoSite:21609022 PhosphoSite:23882028 PhosphoSite:16946300 PhosphoSite:22115753 PhosphoSite:18088087 PhosphoSite:20097925 PhosphoSite:16464493 PhosphoSite:25814448 PhosphoSite:28784162 PhosphoSite:9822689 PhosphoSite:19658100 PhosphoSite:23186163 PhosphoSite:21437283 PhosphoSite:27595981 PhosphoSite:17016520 PhosphoSite:22461510 PhosphoSite:18691976 PhosphoSite:21403953 PhosphoSite:24667141 PhosphoSite:19901323 PhosphoSite:27251275 PhosphoSite:19534553 PhosphoSite:22617229 PhosphoSite:20736484 PhosphoSite:20090780 PhosphoSite:23438512 PhosphoSite:23836654 PhosphoSite:10644735 PhosphoSite:17360941 PhosphoSite:21552520 PhosphoSite:19369195 PhosphoSite:23911959 PhosphoSite:21376226 PhosphoSite:22964225 PhosphoSite:21253578 PhosphoSite:34132782 PhosphoSite:22366891 PhosphoSite:23716303 PhosphoSite:21394647 PhosphoSite:24927040 PhosphoSite:20541700 PhosphoSite:16497976
HCK	P08631	P73	O15350	Yes	No	No	iPTMnetProtMapperRLIMS-P_ProtMapperdbPTMPhosphoSitePhosphoSite_ProtMapper	dbPTM:17535448 PhosphoSite:17535448 ProtMapper:17535448

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Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	HCK	P08631	2	PDB	PDB:3vrzPDB:3vryPDB:2c0iPDB:2c0tPDB:3vs6PDB:3vs5PDB:5zj6PDB:1bu1PDB:3vs0PDB:3vs7PDB:2hckPDB:3vs3PDB:3vs1PDB:3vs4PDB:2c0oPDB:1ad5PDB:4luePDB:4ludPDB:3vs2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryR Sequencing	1	30865381

Sequence, Structure & Domains15

Sequences

Length

526

Mass

59,600

Sequence

MGGRSSCEDPGCPRDEERAPRMGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP

Alternative Products

Event=Alternative splicing, Alternative initiation; Named isoforms=4; Name=1; Synonyms=p60-HCK, p61Hck; IsoId=P08631-1; Sequence=Displayed; Name=2; Synonyms=p59-HCK, p59Hck; IsoId=P08631-2; Sequence=VSP_018858; Name=3; IsoId=P08631-3; Sequence=VSP_018858, VSP_041926; Name=4; IsoId=P08631-4; Sequence=VSP_041926

Alternative Sequence

1..21; Missing (in isoform 2 and isoform 3); 76; Missing (in isoform 3 and isoform 4)

3D Structural Models

Turn

189..191; 252..255; 282..284; 452..454; 513..518

Helix

130..132; 136..138; 139..141; 151..159; 219..228; 259..261; 299..309; 341..345; 350..352; 355..374; 384..386; 402..405; 410..413; 421..423; 426..431; 436..451; 463..471; 484..493; 498..500; 504..512

Beta Strand

82..85; 93..96; 99..102; 104..111; 114..122; 127..129; 133..135; 145..148; 168..172; 174..176; 180..188; 192..202; 204..206; 208..211; 215..218; 233..235; 262..270; 272..281; 285..292; 294..297; 320..324; 326..328; 330..333; 387..389; 395..397; 416..419; 457..460; 480..482; 520..522

3D Structure

NMR spectroscopy (5); X-ray crystallography (35)

Domain & Motif Annotations

Compositional Bias

11..20; Basic and acidic residues

Domain (CC)

The SH3 domain mediates binding to HIV-1 Nef.

Domain (FT)

78..138; SH3; 144..241; SH2; 262..515; Protein kinase

Region

1..20; Disordered

Protein Families (3)

Protein kinase superfamily
Tyr protein kinase family
SRC subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Clinical Relevance8

Disease Involvement

Proto-oncogene

Related Diseases

Indeterminate colitis

Biomarker

Discontinued in Phase 2

Antibody (3)

CAB005195 HPA035534 HPA063768

Interaction Protein (5)

ENSG00000096384ENSG00000121774ENSG00000143537ENSG00000146648ENSG00000155849

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications4

PMID	Title	Abstract
38037300	Proteomic profiling of paired human liver homogenate and tissue derived extracellular vesicles.	No abstract available
39702169	iTRAQ proteomic analysis of exosomes derived from synovial fluid reveals disease patterns and potential biomarkers of Osteoarthritis.	No abstract available
40596376	Proteomic profiling of plasma extracellular vesicles identifies signatures of innate immunity, coagulation, and endothelial activation in septic patients.	No abstract available
40689422	Defining the Ovarian Cancer Precancerous Landscape through Modeling Fallopian Tube Epithelium Reprogramming Driven by Extracellular Vesicles.	No abstract available