EV MemProt Atlas

Basic Information11

Protein Names

SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000158092

Entrez Gene Symbol

NCK1

Gene Synonym (2)

NCKNCKalpha

Gene Description

NCK adaptor protein 1

Chromosome

3

Position

136862208-136951606

Supporting publications (n)

1

EVMP confidence score

0.38

Fluorescence & Localization5

Tissue Specificblood vesselCell SpecificAstrocytesSingle-Nuclei Brain SpecificastrocyteBlood Cell SpecificT-reg

Function & Pathway6

Protein Function

Predicted intracellular proteins

Cellular Component (9)

Molecular Function (13)

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KEGG (5)

Reactome (34)

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Mediation Categories (5)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence31

Enzyme-Mediated Modification (4)

4 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
NCK1	ABL1	P00519	Y	105	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	SIGNOR:22327338 ProtMapper:22327338
NCK1	EGFR	P00533	Y	105	phosphorylation	BEL-Large-Corpus_ProtMapperProtMapper	ProtMapper:15951569
NCK1	EGF	P01133	S	85	phosphorylation	BEL-Large-Corpus_ProtMapperProtMapper	ProtMapper:17081983
NCK1	EGF	P01133	S	91	phosphorylation	BEL-Large-Corpus_ProtMapperProtMapper	ProtMapper:17081983

Ligand-Receptor Signaling (4)

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (15)

15 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
ABL1	P00519	NCK1	P16333	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperHINTSignaLink3Lit-BM-17DOMINOHPRDPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointiPTMnetSIGNOR_ProtMapperSPIKE_LCSPIKE	Lit-BM-17:17474147 ProtMapper:22327338 SPIKE:17474147 SPIKE_LC:11494134 SIGNOR:22327338 Lit-BM-17:10967110 HPRD:7926767 SPIKE_LC:7926767 Lit-BM-17:10372803 SPIKE_LC:20841568 HPRD:12379115 SPIKE_LC:17474147 SignaLink3:23331499 HPRD:11494134 SPIKE:11494134 SignaLink3:11494134 HINT:7926767 Lit-BM-17:7926767 SPIKE:20841568 SPIKE:7926767 Lit-BM-17:11494134 DOMINO:17474147
NCK1	P16333	ABL1	P00519	Yes	Yes	Yes	DOMINOWangSIGNORHPRDCui2007HINTCA1SPIKE_LCLit-BM-17SPIKE	Lit-BM-17:17474147 SPIKE_LC:11494134 SPIKE:17474147 Lit-BM-17:10967110 HPRD:7926767 CA1:10488144 SPIKE_LC:7926767 Lit-BM-17:10372803 SPIKE_LC:20841568 HPRD:12379115 SIGNOR:11494134 SPIKE_LC:17474147 HPRD:11494134 SPIKE:11494134 HINT:7926767 Lit-BM-17:7926767 SPIKE:20841568 SPIKE:7926767 Lit-BM-17:11494134 DOMINO:17474147
NCK1	P16333	PAK1	Q13153	Yes	Yes	No	DOMINOWangNetPathSIGNORELMCui2007HINTHPRDCA1SignaLink3InnateDBLMPIDSPIKE_LCSPIKETCRcuration_SignaLink3	SignaLink3:23109003 CA1:8824201 SignaLink3:9024622 HPRD:10330411 NetPath:9755165 SIGNOR:8824201 SPIKE_LC:9755165 ELM:8798379 InnateDB:11278553 NetPath:10026169 SPIKE:10805734 CA1:11096073 CA1:9024622 SPIKE_LC:8798379 SIGNOR:10026169 SPIKE:8798379 SIGNOR:11157752 HINT:33961781 DOMINO:10805734 DOMINO:15784622 HINT:15784622 HINT:8824201 HINT:11278553 HINT:16490784 SPIKE:9755165 SPIKE_LC:10805734 HPRD:8824201 CA1:9755165 InnateDB:16490784 LMPID:8798379 HPRD:11278241
PAK1	Q13153	NCK1	P16333	Yes	Yes	No	DOMINOWangNetPathPhosphoPointELMCui2007HPRDHINTSignaLink3InnateDBLMPIDSPIKE_LCSPIKE	HPRD:10330411 NetPath:9755165 SPIKE_LC:9755165 ELM:8798379 InnateDB:11278553 NetPath:10026169 SPIKE:10805734 SignaLink3:11157752 SignaLink3:8824201 SignaLink3:23331499 HINT:33961781 DOMINO:10805734 DOMINO:15784622 HINT:15784622 HINT:8824201 HINT:11278553 HINT:16490784 SPIKE:9755165 SPIKE_LC:10805734 HPRD:8824201 InnateDB:16490784 LMPID:8798379 SignaLink3:10026169 HPRD:11278241
CD3E	P07766	NCK1	P16333	Yes	Yes	No	WangNetPathSIGNORELMHINTHPRDIntActLMPIDSPIKE_LCLit-BM-17SPIKE	SPIKE:12110186 IntAct:17617578 Lit-BM-17:12110186 SIGNOR:12110186 HINT:12110186 LMPID:17617578 IntAct:18955169 HPRD:12110186 ELM:18644376 HINT:17617578 Lit-BM-17:17617578 Lit-BM-17:18955169 SPIKE_LC:12110186 NetPath:12110186 ELM:17617578 HINT:18955169
EGFR	P00533	NCK1	P16333	Yes	Yes	No	DOMINOBEL-Large-Corpus_ProtMapperWangNetPathPhosphoPointSIGNORProtMapperHPRDCui2007HINTSignaLink3IntActCancerCellMapSPIKE_LCLit-BM-17SPIKE	CancerCellMap:9843575 IntAct:8662998 Lit-BM-17:1333047 IntAct:16273093 ProtMapper:15951569 SPIKE_LC:8662998 SignaLink3:1333047 NetPath:9843575 SIGNOR:1333047 HINT:25402006 IntAct:24658140 SPIKE_LC:1333047 Lit-BM-17:8662998 HPRD:9362449 HINT:9362449 DOMINO:16273093 Lit-BM-17:10026169 SignaLink3:23331499 SPIKE:8662998 Lit-BM-17:9362449 Lit-BM-17:24658140 HINT:8662998 HINT:24658140 Lit-BM-17:16273093 IntAct:31980649 Lit-BM-17:25402006 SPIKE:1333047 SignaLink3:10026169 SignaLink3:9362449
PGFRB	P09619	NCK1	P16333	Yes	Yes	No	WangPhosphoPointSIGNORELMHPRDSignaLink3SPIKE_LC	SignaLink3:1333047 SignaLink3:23331499 HPRD:9362449 SignaLink3:10026169 SIGNOR:10026169 SPIKE_LC:16189514 ELM:16636066
EPHB1	P54762	NCK1	P16333	Yes	Yes	No	HPRDWangPhosphoPointSignaLink3	SignaLink3:23331499 SignaLink3:9430661 HPRD:9430661
NCK1	P16333	WASL	O00401	Yes	Yes	No	DOMINOWangSIGNORHPRDCui2007HINTSignaLink3IntActSPIKE_LCLit-BM-17	Lit-BM-17:11340081 Lit-BM-17:17474147 HPRD:11340081 SIGNOR:11340081 Lit-BM-17:26496610 HINT:11340081 HINT:26871637 SPIKE_LC:17474147 HINT:16595635 SignaLink3:23331499 IntAct:17474147 SignaLink3:11340081 HINT:26496610 DOMINO:17474147 HINT:33961781
CD3Z	P20963	NCK1	P16333	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:18408722 SignaLink3:23109003 SignaLink3:15972658 SignaLink3:12110186

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Protein Complex Composition (7)

7 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
HT_DM_Cluster396	NCK1 NCK2 PTPN1 PTPN2	O43639 P16333 P17706 P18031	1:1:1:1	Compleat	Compleat:HC1813	22036573
Ternary complex (Abl1Dok1Nck1)	ABL1 DOK1 NCK1 NCK2	O43639 P00519 P16333 Q99704	1:1:1:1	Compleat	Compleat:HC417	15148308
	ALDH18A1 HAT1 HNRNPA1 HNRNPR ILF2 KHDRBS1 KHDRBS2 KHDRBS3 MOV10 NCK1	O14929 O43390 O75525 P09651 P16333 P54886 Q07666 Q12905 Q5VWX1 Q9HCE1	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5502
	HNRNPR KHDRBS1 KHDRBS2 KHDRBS3 MOV10 NCK1 PRKAR1A PUF60 RRM1 RRM2	O43390 O75525 P10644 P16333 P23921 P31350 Q07666 Q5VWX1 Q9HCE1 Q9UHX1	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7020
	ALDH18A1 HDAC6 HNRNPR KHDRBS1 KHDRBS2 KHDRBS3 MOV10 MYD88 NCK1 PRMT1	O43390 O75525 P16333 P54886 Q07666 Q5VWX1 Q99836 Q99873 Q9HCE1 Q9UBN7	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8528
	BRK1 NCK1 NCKAP1 RAC1 UBC	P0CG48 P16333 P63000 Q8WUW1 Q9Y2A7	1:1:1:1:1	CompleatCFinder	Compleat:HC6109
	NCK1	P16333	2	PDB	PDB:5qu3PDB:5qu1PDB:5qu5PDB:5qu6PDB:5qu7PDB:5qu4PDB:5qua

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Size Exclusion Chromatography	Mass spectrometry	1	28211531

Sequence, Structure & Domains10

Sequences

Length

377

Mass

42,864

Sequence

MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P16333-1; Sequence=Displayed; Name=2; IsoId=P16333-2; Sequence=VSP_043122

Alternative Sequence

1..76; MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLG -> MDWLNVFKDFFS (in isoform 2)

3D Structural Models

Helix

33..35; 53..55; 157..159; 289..299; 349..358

Beta Strand

5..8; 28..31; 38..42; 48..52; 56..58; 99..101; 109..115; 131..138; 142..148; 151..156; 160..162; 168..170; 304..309; 311..313; 316..321; 324..326; 328..335; 338..341; 344..348; 361..363

3D Structure

NMR spectroscopy (4); X-ray crystallography (11)

Domain & Motif Annotations

Domain (CC)

Only the first and third SH3 domains seem to be involved in RASA1-binding; the second SH3 domain and the SH2 domains do not seem to be involved.

Domain (FT)

2..61; SH3 1; 106..165; SH3 2; 190..252; SH3 3; 282..376; SH2

Clinical Relevance7

Related Diseases

Multiple sclerosis

Biomarker

Phase 1

Drugs

AX-024

Antibody (2)

CAB005063 HPA030766

Interaction Protein (23)

ENSG00000015285ENSG00000043462ENSG00000061938ENSG00000071051ENSG00000104814ENSG00000106069ENSG00000106299ENSG00000106976ENSG00000110395ENSG00000112079ENSG00000115904ENSG00000115935ENSG00000136754ENSG00000143322ENSG00000145715ENSG00000146648ENSG00000154310ENSG00000155629ENSG00000162522ENSG00000171475ENSG00000180370ENSG00000198851ENSG00000204131

Interaction Count

23

Interaction Dataset (3)

intact_biogridbiogrid_bioplexintact_biogrid_bioplex

Supporting Publications1

PMID	Title	Abstract
36146834	Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro.	No abstract available

Entry name NCK1	UniProt ID P16333	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification Predicted intracellular proteins