Protein detail

CD33

Myeloid cell surface antigen CD33 (Sialic acid-binding Ig-like lectin 3) (Siglec-3) (gp67) (CD antigen CD33)

Entry name
CD33
UniProt ID
EVMP confidence score
0.38
Supporting publications (n)
5
Transmembrane count
1
Protein classification
CD markersFDA approved drug targetsPlasma proteinsPredicted membrane proteins
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information13
Protein Names
Myeloid cell surface antigen CD33 (Sialic acid-binding Ig-like lectin 3) (Siglec-3) (gp67) (CD antigen CD33)
Protein Class (4)
CD markersFDA approved drug targetsPlasma proteinsPredicted membrane proteins
Protein Function (2)
  • CD markers
  • FDA approved drug targets:Biotech drugs
Transmembrane
260..282; Helical
Transmembrane Count
1
Entrez Gene Symbol
Gene Synonym (4)
FLJ00391p67SIGLEC-3SIGLEC3
Gene Description
CD33 molecule
Chromosome
19
Position
51225064-51243860
Supporting publications (n)
5
EVMP confidence score
0.38
Fluorescence & Localization5
CD33 fluorescence
Cell SpecificNeutrophilsSingle-Nuclei Brain Specificmedium spiny neuronBlood Cell SpecificbasophilBlood Lineage Specificgranulocytes
Function & Pathway7
Relations & Evidence48

Enzyme-Mediated Modification (5)

5 records.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
CD33LCKP06239Y340phosphorylationphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapperSIGNOR:10887109ProtMapper:10887109KEA:10887109KEA:10206955phosphoELM:10887109
CD33SRCP12931Y340phosphorylationphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEAphosphoELMLi2012phosphoELM:10887109KEA:10887109KEA:10206955
CD33PTPN6P29350Y340dephosphorylationHPRDHPRD:10206955
CD33EGFRP00533Y340phosphorylationKEAKEA:17570479
CD33LYNP07948Y340phosphorylationKEAKEA:17570479

Ligand-Receptor Signaling (40)

40 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
receptorreceptorGO_IntercellNoYesNoYesNo
receptorreceptorICELLNETNoYesNoYesNo
receptorreceptorCellTalkDBNoYesNoYesNo
innate_immunereceptorICELLNETNoYesNoYesNo
receptorreceptorOmniPathNoYesNoYesNo
extracellularextracellularDGIdbNoNoNoYesNo
extracellularextracellularOmniPathNoNoNoYesNo
intracellularintracellularComPPINoNoNoYesNo
intracellularintracellularGO_IntercellNoNoNoYesNo
intracellularintracellularUniProt_locationNoNoNoYesNo
Page 1 of 4Next

Regulatory Interaction Network (1)

1 record.

Source Protein SymbolSource UniProt IDTarget Protein SymbolTarget UniProt IDIs DirectedIs StimulationIs InhibitionDatabaseReferences
LCKP06239CD33P20138YesYesNoWangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperPhosphoSite_KEAKEAphosphoELM_KEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapperSIGNOR:10887109ProtMapper:10887109PhosphoSite:10206955KEA:10887109KEA:10206955phosphoELM:10887109PhosphoSite:10887109

Protein Complex Composition (1)

1 record.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
CD33P201384PDBPDB:6d4aPDB:5j06PDB:5ihbPDB:5j0bPDB:6d49PDB:6d48PDB:7aw6

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Protein Organic Solvent PrecipitationMass spectrometry132384937
Sequence, Structure & Domains15

Sequences

Length
364
Mass
39,825
Sequence
MPLLLLLPLLWAGALAMDPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISRDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVHGAIGGAGVTALLALCLCLIFFIVKTHRRKAARTAVGRNDTHPTTGSASPKHQKKSKLHGPTETSSCSGAAPTVEMDEELHYASLNFHGMNPSKDTSTEYSEVRTQ
Alternative Products
Event=Alternative splicing; Named isoforms=3; Name=CD33M; IsoId=P20138-1; Sequence=Displayed; Name=3; IsoId=P20138-2; Sequence=VSP_045364; Name=CD33m; IsoId=P20138-3; Sequence=VSP_046172
Alternative Sequence
13..139; Missing (in isoform CD33m); 309..364; KHQKKSKLHGPTETSSCSGAAPTVEMDEELHYASLNFHGMNPSKDTSTEYSEVRTQ -> VR (in isoform 3)

3D Structural Models

Turn
67..69; 84..89
Helix
49..51; 96..98; 110..112; 203..205
Beta Strand
22..25; 27..32; 37..39; 41..44; 56..62; 73..76; 90..92; 103..105; 114..122; 125..128; 134..139; 146..148; 159..164; 176..191; 194..199; 209..215; 217..220; 222..228
3D Structure
NMR spectroscopy (1); X-ray crystallography (8)

Domain & Motif Annotations

Motif
338..343; ITIM motif 1; 356..361; ITIM motif 2
Domain (CC)
Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Domain (FT)
19..135; Ig-like V-type; 145..228; Ig-like C2-type
Region
290..364; Disordered
Protein Families (2)
  • Immunoglobulin superfamily
  • SIGLEC (sialic acid binding Ig-like lectin) family
Sequence Similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Clinical Relevance9
Supporting Publications5
PMIDTitleAbstract
33592500A Proteomic Approach to Understand the Clinical Significance of Acute Myeloid Leukemia-Derived Extracellular Vesicles Reflecting Essential Characteristics of Leukemia.No abstract available
38014595Proteome and immune responses of extracellular vesicles derived from macrophages infected with the periodontal pathogen Tannerella forsythia.No abstract available
40730843Single urinary extracellular vesicle proteomics identifies complement receptor CD35 as a biomarker for sepsis-associated acute kidney injury.No abstract available
41068253Identification of plasma extracellular vesicle protein biomarkers in diabetic retinopathy progression.No abstract available
41307968Extracellular Vesicles Define Discrete Nano-Based Niches Within the Human Haematopoietic System.No abstract available