Protein detail

EPHA3

Ephrin type-A receptor 3 (EC 2.7.10.1) (EPH-like kinase 4) (EK4) (hEK4) (HEK) (Human embryo kinase) (Tyrosine-protein kinase TYRO4) (Tyrosine-protein kinase receptor ETK1) (Eph-like tyrosine kinase 1)

Entry name EPHA3	UniProt ID P29320	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count 1	Protein classification Cancer-related genesDisease related genesEnzymesPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Protein Class (6)

Cancer-related genesDisease related genesEnzymesPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

Protein Function (7)

Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Cancer-related genes:Candidate cancer biomarkers
Kinases:Tyr protein kinases
Disease related genes

Transmembrane

542..565; Helical

Transmembrane Count

Ensembl

ENSG00000044524

Entrez Gene Symbol

EPHA3

Gene Synonym (5)

ETKETK1HEKHEK4TYRO4

Gene Description

EPH receptor A3

Chromosome

Position

89107621-89482134

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization4

Cell SpecificSyncytiotrophoblastsSecretome LocationSecreted - unknown locationSecretome FunctionReceptor

Function & Pathway8

Protein Function (7)

Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Cancer-related genes:Candidate cancer biomarkers
Kinases:Tyr protein kinases
Disease related genes

Cellular Component (8)

Molecular Function (5)

Biological Process (3)

KEGG

hsa04360 Axon guidance

Reactome (4)

Canonical Pathways (3)

M5880 Naba ecm affiliated
M5885 Naba matrisome associated
M5889 Naba matrisome

Mediation Categories (4)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationReceptor-signaling mediation

Relations & Evidence81

Enzyme-Mediated Modification (3)

3 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
EPHA3	PTPN1	P18031	Y	779	dephosphorylation	SIGNORDEPOD	DEPOD:21135139 SIGNOR:21135139
EPHA3	PTPN1	P18031	Y	779	phosphorylation	SIGNOR_ProtMapperProtMapper	ProtMapper:21135139
EPHA3	LCK	P06239	Y	602	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (66)

66 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
kinase	receptor	Almen2009	No	Yes	Yes	Yes	No
receptor_tyrosine_kinases_rtk	receptor	HPMR	No	Yes	Yes	Yes	No
ephrin	receptor	HPMR	No	Yes	Yes	Yes	No
kinase	receptor	Surfaceome	No	Yes	Yes	Yes	No
eph	receptor	Surfaceome	No	Yes	Yes	Yes	No
growth_factor	receptor	ICELLNET	No	Yes	Yes	Yes	No
growth_factor_ephrins	receptor	ICELLNET	No	Yes	Yes	Yes	No
receptor	receptor	OmniPath	No	Yes	Yes	Yes	No
extracellular	extracellular	HPMR	No	No	Yes	Yes	No
extracellular	extracellular	OmniPath	No	No	Yes	Yes	No

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Regulatory Interaction Network (11)

11 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
EPHA3	P29320	PAK1	Q13153	Yes	Yes	No	REACH_ProtMapperSIGNORSparser_ProtMapperProtMapper	SIGNOR:26944939 ProtMapper:26944939

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	29441425

Sequence, Structure & Domains13

Sequences

Length

983

Mass

110,131

Sequence

MDCQLSILLLLSCSVLDSFGELIPQPSNEVNLLDSKTIQGELGWISYPSHGWEEISGVDEHYTPIRTYQVCNVMDHSQNNWLRTNWVPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNLYYMESDDDHGVKFREHQFTKIDTIAADESFTQMDLGDRILKLNTEIREVGPVNKKGFYLAFQDVGACVALVSVRVYFKKCPFTVKNLAMFPDTVPMDSQSLVEVRGSCVNNSKEEDPPRMYCSTEGEWLVPIGKCSCNAGYEERGFMCQACRPGFYKALDGNMKCAKCPPHSSTQEDGSMNCRCENNYFRADKDPPSMACTRPPSSPRNVISNINETSVILDWSWPLDTGGRKDVTFNIICKKCGWNIKQCEPCSPNVRFLPRQFGLTNTTVTVTDLLAHTNYTFEIDAVNGVSELSSPPRQFAAVSITTNQAAPSPVLTIKKDRTSRNSISLSWQEPEHPNGIILDYEVKYYEKQEQETSYTILRARGTNVTISSLKPDTIYVFQIRARTAAGYGTNSRKFEFETSPDSFSISGESSQVVMIAISAAVAIILLTVVIYVLIGRFCGYKSKHGADEKRLHFGNGHLKLPGLRTYVDPHTYEDPTQAVHEFAKELDATNISIDKVVGAGEFGEVCSGRLKLPSKKEISVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIRLEGVVTKSKPVMIVTEYMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVLWEVMSYGERPYWEMSNQDVIKAVDEGYRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSILDKLIRNPGSLKIITSAAARPSNLLLDQSNVDITTFRTTGDWLNGVWTAHCKEIFTGVEYSSCDTIAKISTDDMKKVGVTVVGPQKKIISSIKALETQSKNGPVPV

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P29320-1; Sequence=Displayed; Name=2; IsoId=P29320-2; Sequence=VSP_002995, VSP_002996

Alternative Sequence

532..539; SFSISGES -> CMYYFNAV (in isoform 2); 540..983; Missing (in isoform 2)

3D Structural Models

Turn

38..41; 610..612; 713..716; 819..821; 824..827

Helix

106..108; 137..139; 154..159; 618..620; 661..674; 707..712; 720..739; 749..751; 765..768; 788..790; 793..798; 803..818; 830..838; 851..860; 865..867; 871..883; 885..889

Beta Strand

29..34; 45..48; 51..58; 64..71; 75..77; 80..83; 96..104; 117..128; 130..132; 141..147; 165..170; 176..200; 621..629; 631..641; 647..654; 685..689; 691..694; 696..700; 752..754; 760..762; 890..892; 898..903

3D Structure

X-ray crystallography (28)

Domain & Motif Annotations

Motif

981..983; PDZ-binding

Domain (FT)

29..207; Eph LBD; 325..435; Fibronectin type-III 1; 436..531; Fibronectin type-III 2; 621..882; Protein kinase; 911..975; SAM

Protein Families (3)

Protein kinase superfamily
Tyr protein kinase family
Ephrin receptor subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Clinical Relevance8

Disease Involvement

Cancer-related genes

Related Diseases (4)

Acute myeloid leukaemia Brain cancer Malignant haematopoietic neoplasm Myelodysplastic syndrome

Biomarker

Phase 2; Phase 1; Phase 1/2

Drugs (9)

IRAK-1/4 INHIBITOR BELIZATINIB TAKINIB VANDETANIB KB-004 IFABOTUZUMAB HESPERADIN COMPOUND 1 [WO2012007375]COMPOUND 7 [WO2012007375]

Antibody (2)

CAB010462 HPA069390

Interaction Protein (2)

ENSG00000135333ENSG00000142627

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications1

PMID	Title	Abstract
29635386	Amniotic Fluid Exosome Proteomic Profile Exhibits Unique Pathways of Term and Preterm Labor.	Exosomes were isolated by differential centrifugation and quantified using nanocrystals (Qdot) coupled to CD63 and placental alkaline phosphatase (PLAP) by fluorescence nanoparticle tracking analysis.