EV MemProt Atlas

Basic Information13

Protein Names

Ephrin type-B receptor 2 (EC 2.7.10.1) (Developmentally-regulated Eph-related tyrosine kinase) (ELK-related tyrosine kinase) (EPH tyrosine kinase 3) (EPH-like kinase 5) (EK5) (hEK5) (Renal carcinoma antigen NY-REN-47) (Tyrosine-protein kinase TYRO5) (Tyrosine-protein kinase receptor EPH-3) [Cleaved into: EphB2/CTF1; EphB2/CTF2]

Protein Class (8)

Cancer-related genesDisease related genesEnzymesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

Protein Function (8)

Human disease related genes:Cardiovascular diseases:Hematologic diseases
Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Cancer-related genes:Candidate cancer biomarkers
Kinases:Tyr protein kinases
Disease related genes

Transmembrane

544..564; Helical

Transmembrane Count

1

Ensembl

ENSG00000133216

Entrez Gene Symbol

EPHB2

Gene Synonym (5)

DRTEPHT3ERKHek5Tyro5

Gene Description

EPH receptor B2

Chromosome

1

Position

22710839-22921500

Supporting publications (n)

2

EVMP confidence score

0.50

Fluorescence & Localization3

Tissue Specificsalivary glandCell SpecificBrain excitatory neurons

Function & Pathway7

Protein Function (8)

Human disease related genes:Cardiovascular diseases:Hematologic diseases
Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Cancer-related genes:Candidate cancer biomarkers
Kinases:Tyr protein kinases
Disease related genes

Cellular Component (14)

Page 1 of 2

Molecular Function (9)

Biological Process (3)

KEGG

hsa04360 Axon guidance

Reactome (6)

Mediation Categories (5)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence97

Enzyme-Mediated Modification (2)

2 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
EPHB2	EPHA1	P21709	Y	596	phosphorylation	Li2012
EPHB2	EFNB2	P52799	Y	504	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:28719605

Ligand-Receptor Signaling (71)

71 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	SignaLink_function	No	Yes	No	Yes	No
ephrin	receptor	Almen2009	No	Yes	No	Yes	No
kinase	receptor	Almen2009	No	Yes	No	Yes	No
receptor_tyrosine_kinases_rtk	receptor	HPMR	No	Yes	No	Yes	No
ephrin	receptor	HPMR	No	Yes	No	Yes	No
kinase	receptor	Surfaceome	No	Yes	No	Yes	No
eph	receptor	Surfaceome	No	Yes	No	Yes	No
growth_factor	receptor	ICELLNET	No	Yes	No	Yes	No
growth_factor_ephrins	receptor	ICELLNET	No	Yes	No	Yes	No
receptor	receptor	OmniPath	No	Yes	No	Yes	No

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Regulatory Interaction Network (15)

15 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
EPHB2	P29323	NRCAM	Q92823	Yes	Yes	No	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	ProtMapper:24023801 SIGNOR:24023801
EPHB2	P29323	RRAS	P10301	Yes	No	Yes	WangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAphosphoELM_KEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSite_ProtMapper	ProtMapper:10570155 SIGNOR:10570155 phosphoELM:10570155 HPRD:10570155 KEA:11682467 KEA:10570155
EPHB2	P29323	ARHGF	O94989	Yes	No	Yes	Sparser_ProtMapperiPTMnetSIGNORProtMapperSIGNOR_ProtMapper	ProtMapper:21029865 ProtMapper:30682817 SIGNOR:21029865
EPHB2	P29323	L1CAM	P32004	Yes	No	No	WangPhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:31640849 PhosphoSite:29109170 PhosphoSite:19720049
EPHB2	P29323	TAGAP	Q8N103	Yes	No	No	Sparser_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:32312989 ProtMapper:32312989

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Protein Complex Composition (8)

8 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
CDC5L EFTUD2 EPHB2 HNRNPM PLRG1 PRPF8 RNPS1 SF3A2 SNRNP200 SNRPB SNW1 SRRM2 SRSF2 TKFC	O43660 O75643 P14678 P29323 P52272 Q01130 Q13573 Q15029 Q15287 Q15428 Q3LXA3 Q6P2Q9 Q99459 Q9UQ35	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC3758
EDF1 EPHB2 FOS JUN PKNOX2 TAF1 TAF13 TBP TBPL1	O60869 P01100 P05412 P20226 P21675 P29323 P62380 Q15543 Q96KN3	1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7965
CDC5L EFTUD2 EPHB2 HNRNPM PRPF8 RNPS1 SF3A2 SNRNP200 SNRNP70 SNRPB SRRM2 SRSF1 SRSF2	O75643 P08621 P14678 P29323 P52272 Q01130 Q07955 Q15029 Q15287 Q15428 Q6P2Q9 Q99459 Q9UQ35	1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4613
DDX18 DDX54 EBNA1BP2 EIF6 EPHB2 FTSJ3 GNL3 GTPBP4 NOC2L NOP2 NSA2 RRP1B RSL1D1 TKFC WDR74	O76021 O95478 P29323 P46087 P56537 Q14684 Q3LXA3 Q6RFH5 Q8IY81 Q8TDD1 Q99848 Q9BVP2 Q9BZE4 Q9NVP1 Q9Y3T9	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5412
EPHB2	P29323	8	PDB	PDB:1b4fPDB:2qbx
EPHB2 SPSB2 TTC1	P29323 Q99614 Q99619	0:0:0	hu.MAPhu.MAP2
EPHB2 SPSB2	P29323 Q99619	0:0	hu.MAPhu.MAP2
EPHB2 KLHDC2	P29323 Q9Y2U9	2:2	PDB	PDB:8ebl

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Small R sequencing (Illumi HiSeq 2000 (Solexa)Mass spectrometry	1	38617517

Sequence, Structure & Domains15

Sequences

Length

1,055

Mass

117,493

Sequence

MALRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIQEKLPLIIGSSAAGLVFLIAVVVIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGINLPLLDRTIPDYTSFNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMNQIQSVEGQPLARRPRATGRTKRCQPRDVTKKTCNSNDGKKKGMGKKKTDPGRGREIQGIFFKEDSHKESNDCSCGG

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; Synonyms=EPHB2v, Long; IsoId=P29323-1; Sequence=Displayed; Name=2; Synonyms=Short; IsoId=P29323-2; Sequence=VSP_003016, VSP_003017; Name=3; IsoId=P29323-3; Sequence=VSP_015713, VSP_003016, VSP_003017

Alternative Sequence

568; R -> RR (in isoform 3); 986; G -> V (in isoform 2 and isoform 3); 987..1055; Missing (in isoform 2 and isoform 3)

3D Structural Models

Turn

713..716; 826..829

Helix

26..28; 97..99; 618..620; 661..674; 707..712; 720..739; 749..751; 790..792; 795..800; 805..820; 832..840; 853..862; 873..885; 887..890; 918..924; 928..930; 931..936; 942..945; 950..955; 961..982; 1050..1052

Beta Strand

21..25; 36..39; 44..49; 55..61; 66..68; 71..74; 84..94; 111..120; 130..132; 135..141; 148..152; 155..166; 171..183; 185..194; 621..626; 635..640; 648..655; 685..689; 691..700; 752..754; 760..762

3D Structure

X-ray crystallography (5)

Domain & Motif Annotations

Compositional Bias

991..1002; Basic residues; 1025..1049; Basic and acidic residues

Motif

984..986; PDZ-binding (in isoform 2)

Domain (FT)

20..202; Eph LBD; 324..434; Fibronectin type-III 1; 435..530; Fibronectin type-III 2; 621..884; Protein kinase; 913..977; SAM

Region

990..1055; Disordered

Protein Families (3)

Protein kinase superfamily
Tyr protein kinase family
Ephrin receptor subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Clinical Relevance8

Disease Involvement (3)

Cancer-related genesDisease variantTumor suppressor

Related Diseases (4)

Kaposi sarcoma Melanoma Pancreatic cancer Solid tumour/cancer

Biomarker

Phase 2; Phase 1; Phase 1/2

Drugs (76)

AZ960 PF-06263276 SOLCITINIB GSK2646264 -SAR 20347.00 IVARMACITINIB JAK INHIBITOR 17B KO-947 XL019 RG-1530 REPOTRECTINIB HG-10-102-01 ILGINATINIB MK-8353 DEURUXOLITINIB EMZELTRECTINIB ABROCITINIB FILGOTINIB ULIXERTINIB TINENGOTINIB JAK INHIBITOR 20A SAFIMALTIB ROVADICITINIB SJ988497 IZENCITINIB PEFICITINIB MOMELOTINIB CEE321 UPADACITINIB CEP-33779 COMPOUND 8L [PMID: 36053746]AC430 COMPOUND 1D [PMID: 21493067]FEDRATINIB LASW1393 TYK2 INHIBITOR 14L PACRITINIB DECERNOTINIB ITACNOSERTIB PF-956980 JAK3 INHIBITOR 34 GUSACITINIB AZD1480 COMPOUND 18E [PMID: 31670517]COMPOUND 19A [PMID: 24359159]COMPOUND 30 [PMID: 37057760]AT-9283 LORPUCITINIB RUXOLITINIB OCLACITINIB TOFACITINIB DELGOCITINIB BIIB068 COMPOUND 13AC [PMID: 33256400]BARICITINIB LONDAMOCITINIB JAK3 INHIBITOR 32 CERDULATINIB BMS-911543 BREPOCITINIB NEZULCITINIB ROPSACITINIB RGB-286638 GDC-0214 VANDETANIB COMPOUND 11 [PMID: 35653642]COMPOUND 25AP [PMID: 37796543]GANDOTINIB 2-TERT-BUTYL-9-FLUORO-1,6-DIHYDROBENZO[H]IMIDAZO[4,5-F]ISOQUINOLIN-7-ONE ZOTIRACICLIB WXFL10203614 GOLIDOCITINIB COMPOUND 13 [PMID: 35653642]JNJ-7706621 JAK3 INHIBITOR II ITACITINIB

Antibody (2)

CAB013647 HPA071200

Interaction Protein (3)

ENSG00000116106ENSG00000135333ENSG00000182578

Interaction Count

3

Interaction Dataset

intact_biogrid

Supporting Publications2

PMID	Title	Abstract
32560723	T2 and T17 cytokines alter the cargo and function of airway epithelium-derived extracellular vesicles.	No abstract available
40189497	Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.	No abstract available

Entry name EPHB2	UniProt ID P29323	EVMP confidence score 0.50
Supporting publications (n) 2	Transmembrane count 1	Protein classification Cancer-related genesDisease related genesEnzymesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins