EV MemProt Atlas

Basic Information

Protein Names

Serine/threonine-protein kinase N1 (EC 2.7.11.13) (Protease-activated kinase 1) (PAK-1) (Protein kinase C-like 1) (Protein kinase C-like PKN) (Protein kinase PKN-alpha) (Protein-kinase C-related kinase 1) (Serine-threonine protein kinase N)

Protein Class

EnzymesPredicted intracellular proteins

Protein Function

ENZYME proteins:Transferases
Enzymes
Kinases:AGC Ser/Thr protein kinases
Predicted intracellular proteins

Ensembl

ENSG00000123143

Entrez Gene Symbol

PKN1

Gene Synonym

DBKMGC46204PAK1PKNPRK1PRKCL1

Gene Description

Protein kinase N1

Chromosome

19

Position

14433053-14471867

Frequency

1

EVMP Score

0.50

Fluorescence & Localization

Tissue SpecificintestineBrain Regional SpecificcerebellumCell SpecificEnterocytesBlood Cell SpecificneutrophilBlood Lineage Specificgranulocytes

Function & Pathway

Protein Function

ENZYME proteins:Transferases
Enzymes
Kinases:AGC Ser/Thr protein kinases
Predicted intracellular proteins

Cellular Component

Molecular Function

Biological Process

KEGG

Reactome

Mediation Categories

Clinical-translation mediationImmune mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

9 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
PKN1	PDPK1	O15530	T	774	phosphorylation	SIGNORProtMapperHPRDdbPTMKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	dbPTM:10792047 KEA:10792047 ProtMapper:10753910 HPRD:18669648 HPRD:10753910 KEA:10753910 SIGNOR:10753910 HPRD:10792047 phosphoELM:10792047
PKN1	PDPK1	O15530	T	780	phosphorylation	HPRDKEA	HPRD:10792047 KEA:10753910 KEA:10792047 HPRD:18669648
PKN1	PDPK1	O15530	S	773	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
PKN1	CDK1	P06493	S	537	phosphorylation	PhosphoSite
PKN1	CDK1	P06493	S	562	phosphorylation	PhosphoSite
PKN1	CDK1	P06493	S	916	phosphorylation	PhosphoSiteKEA	KEA:17570479
PKN1	CDK1	P06493	S	533	phosphorylation	PhosphoSite
PKN1	PDK1	Q15118	T	780	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PKN1	PDK1	Q15118	T	774	phosphorylation	KEA	KEA:10792047 KEA:10753910

Ligand-Receptor Signaling

9 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network

18 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
PKN1	Q16512	FZD7	O75084	Yes	Yes	No	SignaLink3	SignaLink3:24114839
CDK1	P06493	PKN1	Q16512	Yes	Yes	No	Sparser_ProtMapperPhosphoSite_ProtMapperPhosphoSite_norefSIGNORProtMapperKEAWangREACH_ProtMapperPhosphoSiteNetworKIN_KEA	KEA:17570479 SIGNOR:31981797 PhosphoSite:31981797 ProtMapper:31981797
PKN1	Q16512	RAF1	P04049	Yes	Yes	No	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	ProtMapper:15849194 ProtMapper:11733498 SIGNOR:15849194 SIGNOR:11733498
PDPK1	O15530	PKN1	Q16512	Yes	Yes	No	SIGNORProtMapperdbPTMPhosphoSite_KEAInnateDBLit-BM-17HPRDLMPIDPhosphoSite_ProtMapperiPTMnetPhosphoPointELMKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSiteSPIKE_LCHPRD-phos	PhosphoSite:26296974 dbPTM:10792047 HPRD-phos:18669648 ELM:10764742 KEA:10753910 HPRD:10792047 HPRD-phos:10792047 ProtMapper:10753910 HPRD:10753910 LMPID:10764742 PhosphoSite:10792047 HPRD:10764742 Lit-BM-17:10764742 KEA:10792047 PhosphoSite:10753910 PhosphoSite:10467162 ProtMapper:10792047 HPRD-phos:10753910 SIGNOR:10753910 ProtMapper:18669648 phosphoELM:10792047 SPIKE_LC:16189514 InnateDB:10764742
PKN1	Q16512	PGM1	P36871	Yes	Yes	No	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	SIGNOR:15378030 ProtMapper:15378030
PKN1	Q16512	ARHG2	Q92974	Yes	No	Yes	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	SIGNOR:14970201 ProtMapper:14970201
PKN1	Q16512	EGFR	P00533	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperSIGNOR_ProtMapperPhosphoSite_ProtMapper	SIGNOR:21749319 ProtMapper:21749319
PKN1	Q16512	MPIP3	P30307	Yes	No	No	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDRLIMS-P_ProtMapperPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperHPRD-phos	HPRD-phos:11551930 KEA:10557092 KEA:9543386 HPRD-phos:9543386 ProtMapper:9278512 SIGNOR:15791647 KEA:11551930 HPRD-phos:10557092 ProtMapper:10557092 KEA:9278511 KEA:9836640 HPRD-phos:20068231 KEA:15791647 HPRD:15791647 KEA:9278512 KEA:10744722 ProtMapper:11333986 ProtMapper:11551930 HPRD-phos:9278512 HPRD-phos:11333986 KEA:11333986 KEA:15629715 ProtMapper:20068231 KEA:12941695 KEA:15150265 ProtMapper:15791647 ProtMapper:9543386 KEA:10681541
PKN1	Q16512	ANDR	P10275	Yes	Yes	No	WangSIGNOR	SIGNOR:17251915
PKN1	Q16512	MEFV	O15553	Yes	No	Yes	Sparser_ProtMapperPhosphoSite_norefSIGNORProtMapperREACH_ProtMapperPhosphoSite_ProtMapper	ProtMapper:31177443 SIGNOR:27270401 ProtMapper:29178647 ProtMapper:33338767 ProtMapper:27270401 ProtMapper:31456795 ProtMapper:32962268

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Protein Complex Composition

8 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
AKAP13-MAP2K3-MAP3K20-MAPK14-PKN1 complex	AKAP13 MAP2K3 MAP3K20 MAPK14 PKN1	P46734 Q12802 Q16512 Q16539 Q9NYL2	0:0:0:0:0	CORUM	CORUM:7593	21224381
	MAP2K3 MAP2K4 MAP2K6 MAP3K4 MAP3K5 MAPK12 MAPK13 MAPK14 MAPKAPK5 PKN1 TAOK2 UBC	O15264 P0CG48 P45985 P46734 P52564 P53778 Q16512 Q16539 Q8IW41 Q99683 Q9UL54 Q9Y6R4	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6501
	MAP2K3 MAP2K4 MAP2K6 MAP3K10 MAP3K4 MAPK12 MAPK14 NEFL PKN1 TAOK2	P07196 P45985 P46734 P52564 P53778 Q02779 Q16512 Q16539 Q9UL54 Q9Y6R4	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5486
	MAP2K3 MAP2K6 MAP3K5 MAPK12 MAPK14 MAPKAPK5 PKN1 RPL13 TAOK2 UBC	P0CG48 P26373 P46734 P52564 P53778 Q16512 Q16539 Q8IW41 Q99683 Q9UL54	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4610
	MAP2K3 MAP2K4 MAP2K6 MAP3K4 MAP3K5 MAPK12 MAPK14 PKN1 TAOK2	P45985 P46734 P52564 P53778 Q16512 Q16539 Q99683 Q9UL54 Q9Y6R4	1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6705
	PKN1 RAC1	P63000 Q16512	1:1	PDB	PDB:2rmk
	ATP7A COG6 KIF5A PKN1	Q04656 Q12840 Q16512 Q9Y2V7	0:0:0:0	hu.MAP2
	ATP7A COG6 NAV1 PKN1	Q04656 Q16512 Q8NEY1 Q9Y2V7	0:0:0:0	hu.MAP2

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	4	32560723 38207106 38716512 32854315

Sequence, Structure & Domains

Sequences

Length

942

Mass

103,932

Sequence

MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q16512-1; Sequence=Displayed; Name=2; IsoId=Q16512-2; Sequence=VSP_038143; Name=3; IsoId=Q16512-3; Sequence=VSP_039213, VSP_039214

Alternative Sequence

1..7; MASDAVQ -> MAEANNPSEQELE (in isoform 2); 603; S -> R (in isoform 3); 604..942; Missing (in isoform 3)

3D Structural Models

Turn

122..124; 157..159; 635..637; 672..674; 707..709; 854..856; 862..864; 931..934

Helix

15..18; 29..66; 71..95; 126..151; 160..187; 612..614; 648..653; 657..671; 703..706; 714..733; 743..745; 779..781; 784..788; 794..810; 820..829; 840..849; 865..869; 872..874; 879..883; 906..909; 926..930

Beta Strand

194..196; 615..624; 627..634; 640..647; 681..686; 688..696; 700..702; 746..748; 750..752; 754..756; 763..765

3D Structure

NMR spectroscopy (2); X-ray crystallography (6)

Domain & Motif Annotations

Compositional Bias

581..596; Polar residues

Domain (CC)

The C1 domain does not bind the diacylglycerol (DAG).

Domain (FT)

25..100; REM-1 1; 112..193; REM-1 2; 201..280; REM-1 3; 307..470; C2; 615..874; Protein kinase; 875..942; AGC-kinase C-terminal

Region

102..121; Disordered; 181..185; Important for interaction with bacterial SspH1 and SspH1-mediated polyubiquitination; 341..363; Disordered; 553..603; Disordered

Protein Families

Protein kinase superfamily
AGC Ser/Thr protein kinase family
PKC subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Clinical Relevance

Drug Targets

Literature-reported target

Drugs

QUERCETIN INGENOL MEBUTATE

Antibody

HPA003982 CAB010278

Interaction Protein

ENSG00000026025ENSG00000056558ENSG00000127191ENSG00000140743ENSG00000175602ENSG00000204713ENSG00000277586

Interaction Count

7

Interaction Dataset

intact_biogrid

Entry name PKN1	UniProt ID Q16512	EVMP score 0.50
Frequency 1	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins