Protein detail

KGUA

Guanylate kinase (EC 2.7.4.8) (GMP kinase) (Guanylate kinase 1)

Entry name KGUA	UniProt ID Q16774	EVMP confidence score 0.75
Supporting publications (n) 23	Transmembrane count	Protein classification EnzymesEssential proteinsMetabolic proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information10

Protein Names

Guanylate kinase (EC 2.7.4.8) (GMP kinase) (Guanylate kinase 1)

Protein Class (4)

EnzymesEssential proteinsMetabolic proteinsPredicted intracellular proteins

Protein Function (3)

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins

Ensembl

ENSG00000143774

Entrez Gene Symbol

GUK1

Gene Description

Guanylate kinase 1

Chromosome

Position

228139962-228148984

Supporting publications (n)

EVMP confidence score

0.75

Fluorescence & Localization3

Tissue SpecificbrainCell SpecificAstrocytesSingle-Nuclei Brain Specificastrocyte

Function & Pathway7

Protein Function (3)

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins

Cellular Component (3)

Molecular Function (3)

Biological Process (3)

KEGG (3)

Reactome (4)

Mediation Categories (3)

Clinical-translation mediationFusion and delivery mediationMetabolism mediation

Relations & Evidence7

Ligand-Receptor Signaling (5)

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	GUK1	Q16774	2	PDB	PDB:8pts

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow Filtration	Mass spectrometry	1	40595564

Sequence, Structure & Domains12

Sequences

Length

197

Mass

21,726

Sequence

MSGPRPVVLSGPSGAGKSTLLKRLLQEHSGIFGFSVSHTTRNPRPGEENGKDYYFVTREVMQRDIAAGDFIEHAEFSGNLYGTSKVAVQAVQAMNRICVLDVDLQGVRNIKATDLRPIYISVQPPSLHVLEQRLRQRNTETEESLVKRLAAAQADMESSKEPGLFDVVIINDSLDQAYAELKEALSEEIKKAQRTGA

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q16774-1; Sequence=Displayed; Name=2; IsoId=Q16774-2; Sequence=VSP_043778; Name=3; IsoId=Q16774-3; Sequence=VSP_043778, VSP_047372

Alternative Sequence

1; M -> MLRRPLAGLAAAALGRAPPDGM (in isoform 2 and isoform 3); 159..197; SKEPGLFDVVIINDSLDQAYAELKEALSEEIKKAQRTGA -> RNQESSKDRRLRLAVCSRHPGPIQDQGSSIEPPPWQAIRQLCALGQHVEWRRCCPCGWNILG (in isoform 3)

3D Structural Models

Turn

29..31; 49..51; 162..164

Helix

17..27; 58..67; 85..93; 104..111; 127..137; 142..158; 174..193

Beta Strand

7..11; 12..14; 32..34; 38..41; 70..76; 79..84; 97..101; 118..123; 166..170

3D Structure

NMR spectroscopy (1); X-ray crystallography (3)

Domain & Motif Annotations

Domain (FT)

4..186; Guanylate kinase-like

Protein Families

Guanylate kinase family

Sequence Similarities

Belongs to the guanylate kinase family.

Clinical Relevance2

Disease Involvement

Disease variant

Antibody

HPA048587

Supporting Publications23

PMID	Title	Abstract
40619995	A 96-Well Ultrafiltration Approach for the High-Throughput Proteome Analysis of Extracellular Vesicles Isolated From Conditioned Medium.	No abstract available
41227296	Extracellular Vesicles Profiling in Acute Myeloid Leukemia Cell Lines: A Proteomic Characterization.	No abstract available
41315767	Multi-omics identify hallmark protein and lipid features of small extracellular vesicles circulating in human plasma.	No abstract available

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