Protein detail

OTU1

Ubiquitin thioesterase OTU1 (EC 3.4.19.12) (DUBA-8) (HIV-1-induced protease 7) (HIN-7) (HsHIN7) (OTU domain-containing protein 2)

Entry name OTU1	UniProt ID Q5VVQ6	EVMP confidence score 0.50
Supporting publications (n) 8	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Ubiquitin thioesterase OTU1 (EC 3.4.19.12) (DUBA-8) (HIV-1-induced protease 7) (HIN-7) (HsHIN7) (OTU domain-containing protein 2)

Protein Class (2)

EnzymesPredicted intracellular proteins

Protein Function (3)

Enzymes
Predicted intracellular proteins
ENZYME proteins:Hydrolases

Ensembl

ENSG00000180667

Entrez Gene Symbol

YOD1

Gene Synonym (3)

DKFZp451J1719DUBA8OTUD2

Gene Description

YOD1 deubiquitinase

Chromosome

Position

207043849-207052980

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization2

Tissue Specificbone marrowCell SpecificErythrocyte progenitors

Function & Pathway7

Protein Function (3)

Enzymes
Predicted intracellular proteins
ENZYME proteins:Hydrolases

Cellular Component (2)

Molecular Function (7)

Biological Process (3)

KEGG

hsa04141 Protein processing in endoplasmic reticulum

Reactome (3)

Mediation Categories (2)

Fusion and delivery mediationMetabolism mediation

Relations & Evidence32

Ligand-Receptor Signaling (4)

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Protein Complex Composition (27)

27 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
CREBBP-KAT2B-MYOD1 complex	CREBBP KAT2B MYOD1	P15172 Q92793 Q92831	0:0:0	CORUM	CORUM:2641	9659901
MYOD/E12E47	MYOD1 TCF3	P15172 P15923	1:1	SIGNORCompleat	Compleat:HC550SIGNOR:SIGNOR-C127	9184158
MYOD/E2-2	MYOD1 TCF4	P15172 P15884	0:0	SIGNOR	SIGNOR:SIGNOR-C129
MYOD/HEB	MYOD1 TCF12	P15172 Q99081	0:0	SIGNOR	SIGNOR:SIGNOR-C128
MYOD1/SWI/SNF complex	ACTL6A ARID1A ARID1B ARID2 MYOD1 PBX1 SMARCA4 SMARCB1 SMARCC1 SMARCC2 SMARCD1 SMARCE1	O14497 O96019 P15172 P40424 P51532 Q12824 Q68CP9 Q8NFD5 Q8TAQ2 Q92922 Q969G3 Q96GM5	0:0:0:0:0:0:0:0:0:0:0:0	SIGNOR	SIGNOR:SIGNOR-C93
VCP-YOD1 AAA ATPase complex	MYOD1 VCP	P15172 P55072	1:1	ComplexPortal	intact:EBI-46424859	36825201 19818707 14755292 28819009
p300/CBP-PCAF-MyoD complex	CREBBP EP300 KAT2B MYOD1	P15172 Q09472 Q92793 Q92831	1:1:1:1	Compleat	Compleat:HC462	9659901
tRNA-intron endonuclease complex	MYOD1 TSEN2 TSEN34 TSEN54	P15172 Q7Z6J9 Q8NCE0 Q9BSV6	1:1:1:1	Compleat	Compleat:HC2585	9200603
	CLP1 EEFSEC GAK MYOD1 TSEN2 TSEN34 TSEN54 UBC	O14976 P0CG48 P15172 P57772 Q7Z6J9 Q8NCE0 Q92989 Q9BSV6	1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5305
	CLP1 GAK MYOD1 TSEN2 TSEN34 TSEN54 TUFM	O14976 P15172 P49411 Q7Z6J9 Q8NCE0 Q92989 Q9BSV6	1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC3988

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Western blottingFlow cytometry	1	38321535

Sequence, Structure & Domains14

Sequences

Length

348

Mass

38,322

Sequence

MFGPAKGRHFGVHPAPGFPGGVSQQAAGTKAGPAGAWPVGSRTDTMWRLRCKAKDGTHVLQGLSSRTRVRELQGQIAAITGIAPGGQRILVGYPPECLDLSNGDTILEDLPIQSGDMLIIEEDQTRPRSSPAFTKRGASSYVRETLPVLTRTVVPADNSCLFTSVYYVVEGGVLNPACAPEMRRLIAQIVASDPDFYSEAILGKTNQEYCDWIKRDDTWGGAIEISILSKFYQCEICVVDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQRNFPDPDTPPLTIFSSNDDIVLVQALELADEARRRRQFTDVNRFTLRCMVCQKGLTGQAEAREHAKETGHTNFGEV

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q5VVQ6-1; Sequence=Displayed; Name=2; IsoId=Q5VVQ6-2; Sequence=VSP_024122

Alternative Sequence

1..61; MFGPAKGRHFGVHPAPGFPGGVSQQAAGTKAGPAGAWPVGSRTDTMWRLRCKAKDGTHVLQ -> METLHIIYSEAKSFTVE (in isoform 2)

3D Structural Models

Turn

194..196; 241..244; 250..252

Helix

138..140; 160..169; 176..178; 179..192; 199..202; 206..213; 222..232; 292..308

Beta Strand

148..151; 215..217; 234..240; 245..249; 256..263; 268..274; 276..280; 285..287

3D Structure

X-ray crystallography (3)

Domain & Motif Annotations

Compositional Bias

1..11; Basic residues

Zinc Finger

318..342; C2H2-type

Domain (CC)

The UBAX-like region mediates the interaction with VCP. According to PubMed:19818707, it corresponds to a UBX domain, which is a hallmark for VCP-associated proteins. However, no canonical UBX is detected by prediction tools such as Pfam or PROSITE.; DOMAIN: The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains (PubMed:23827681).

Domain (FT)

149..274; OTU

Region

1..39; Disordered; 50..128; UBX-like; 154..160; Cys-loop; 213..223; Variable-loop; 263..267; His-loop; 291..296; S2 site

Clinical Relevance4

Antibody (2)

HPA028400 HPA028439

Interaction Protein (4)

ENSG00000150991ENSG00000165280ENSG00000167671ENSG00000175104

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications8

PMID	Title	Abstract
31805958	Proteomic analysis of cerebrospinal fluid extracellular vesicles reveals synaptic injury, inflammation, and stress response markers in HIV patients with cognitive impairment.	No abstract available
32560723	T2 and T17 cytokines alter the cargo and function of airway epithelium-derived extracellular vesicles.	No abstract available
32854315	Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study.	Recent studies have highlighted the importance of Aβ and tau-containing extracellular vesicles (EVs) in AD.
33304478	The proteomic landscape of small urinary extracellular vesicles during kidney transplantation.	No abstract available
33309826	Proteomic analysis of extracellular vesicles and conditioned medium from human adipose-derived stem/stromal cells and dermal fibroblasts.	No abstract available
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available
38607062	Enrichment, Characterization, and Proteomic Profiling of Small Extracellular Vesicles Derived from Human Limbal Mesenchymal Stromal Cells and Melanocytes.	No abstract available
39207047	The trajectory of vesicular proteomic signatures from HBV-HCC by chitosan-magnetic bead-based separation and DIA-proteomic analysis.	No abstract available