Protein detail

OTU1

Ubiquitin thioesterase OTU1 (EC 3.4.19.12) (DUBA-8) (HIV-1-induced protease 7) (HIN-7) (HsHIN7) (OTU domain-containing protein 2)

Protein symbol
OTU1
UniProt ID
EVMP score
0.50
Frequency
8
Transmembrane count
Protein classification
EnzymesPredicted intracellular proteins
Basic Information
Protein Names
Ubiquitin thioesterase OTU1 (EC 3.4.19.12) (DUBA-8) (HIV-1-induced protease 7) (HIN-7) (HsHIN7) (OTU domain-containing protein 2)
Protein Class
EnzymesPredicted intracellular proteins
Protein Function
  • Enzymes
  • Predicted intracellular proteins
  • ENZYME proteins:Hydrolases
Entrez Gene Symbol
Gene Synonym
DKFZp451J1719DUBA8OTUD2
Gene Description
YOD1 deubiquitinase
Chromosome
1
Position
207043849-207052980
Frequency
8
EVMP Score
0.50
Fluorescence & Localization
Tissue Specificbone marrowCell SpecificErythrocyte progenitors
Function & Pathway
Relations & Evidence

Enzyme-Mediated Modification

0 records.

Ligand-Receptor Signaling

4 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo

Regulatory Interaction Network

0 records.

Isolation & Detection Technology

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationWestern blottingFlow cytometry138321535
Sequence, Structure & Domains

Sequences

Length
348
Mass
38,322
Sequence
MFGPAKGRHFGVHPAPGFPGGVSQQAAGTKAGPAGAWPVGSRTDTMWRLRCKAKDGTHVLQGLSSRTRVRELQGQIAAITGIAPGGQRILVGYPPECLDLSNGDTILEDLPIQSGDMLIIEEDQTRPRSSPAFTKRGASSYVRETLPVLTRTVVPADNSCLFTSVYYVVEGGVLNPACAPEMRRLIAQIVASDPDFYSEAILGKTNQEYCDWIKRDDTWGGAIEISILSKFYQCEICVVDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQRNFPDPDTPPLTIFSSNDDIVLVQALELADEARRRRQFTDVNRFTLRCMVCQKGLTGQAEAREHAKETGHTNFGEV
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q5VVQ6-1; Sequence=Displayed; Name=2; IsoId=Q5VVQ6-2; Sequence=VSP_024122
Alternative Sequence
1..61; MFGPAKGRHFGVHPAPGFPGGVSQQAAGTKAGPAGAWPVGSRTDTMWRLRCKAKDGTHVLQ -> METLHIIYSEAKSFTVE (in isoform 2)

3D Structural Models

Turn
194..196; 241..244; 250..252
Helix
138..140; 160..169; 176..178; 179..192; 199..202; 206..213; 222..232; 292..308
Beta Strand
148..151; 215..217; 234..240; 245..249; 256..263; 268..274; 276..280; 285..287
3D Structure
X-ray crystallography (3)

Domain & Motif Annotations

Compositional Bias
1..11; Basic residues
Zinc Finger
318..342; C2H2-type
Domain (CC)
The UBAX-like region mediates the interaction with VCP. According to PubMed:19818707, it corresponds to a UBX domain, which is a hallmark for VCP-associated proteins. However, no canonical UBX is detected by prediction tools such as Pfam or PROSITE.; DOMAIN: The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains (PubMed:23827681).
Domain (FT)
149..274; OTU
Region
1..39; Disordered; 50..128; UBX-like; 154..160; Cys-loop; 213..223; Variable-loop; 263..267; His-loop; 291..296; S2 site
Clinical Relevance
Interaction Protein
ENSG00000150991ENSG00000165280ENSG00000167671ENSG00000175104
Interaction Count
4
Interaction Dataset
intact_biogrid