Protein detail

RHDF2

Inactive rhomboid protein 2 (iRhom2) (Rhomboid 5 homolog 2) (Rhomboid family member 2) (Rhomboid veinlet-like protein 5) (Rhomboid veinlet-like protein 6)

Entry name RHDF2	UniProt ID Q6PJF5	EVMP confidence score 0.38
Supporting publications (n) 6	Transmembrane count 7	Protein classification Disease related genesEnzymesPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Inactive rhomboid protein 2 (iRhom2) (Rhomboid 5 homolog 2) (Rhomboid family member 2) (Rhomboid veinlet-like protein 5) (Rhomboid veinlet-like protein 6)

Protein Class (5)

Disease related genesEnzymesPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

Protein Function (5)

Predicted intracellular proteins
Potential drug targets
Peptidases:Serine-type peptidases
Enzymes
Disease related genes

Transmembrane

410..430; Helical; 661..681; Helical; 693..713; Helical; 716..736; Helical; 748..768; Helical; 774..794; Helical; 803..823; Helical

Transmembrane Count

Ensembl

ENSG00000129667

Entrez Gene Symbol

RHBDF2

Gene Synonym (6)

FLJ22341iRhom2RHBDL5RHBDL6TOCTOCG

Gene Description

Rhomboid 5 homolog 2

Chromosome

Position

76470891-76501790

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization4

Tissue Specificchoroid plexusBrain Regional Specificchoroid plexusCell SpecificBergmann gliaSingle-Nuclei Brain Specificchoroid plexus epithelial cell

Function & Pathway7

Protein Function (5)

Predicted intracellular proteins
Potential drug targets
Peptidases:Serine-type peptidases
Enzymes
Disease related genes

Cellular Component (3)

Molecular Function (3)

Biological Process (3)

KEGG

hsa04668 TNF signaling pathway

Reactome (4)

Mediation Categories (4)

Clinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence26

Ligand-Receptor Signaling (20)

20 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	CellTalkDB	No	Yes	No	Yes	No
receptor	receptor	OmniPath	No	Yes	No	Yes	No
intracellular	intracellular	GO_Intercell	No	No	No	Yes	No
intracellular	intracellular	UniProt_location	No	No	No	Yes	No
intracellular	intracellular	OmniPath	No	No	No	Yes	No
growth_factor_binder	ligand_regulator	UniProt_keyword	Yes	No	No	Yes	No
ligand_regulator	ligand_regulator	OmniPath	Yes	No	No	Yes	No
transmembrane	transmembrane	UniProt_location	No	No	No	Yes	No
transmembrane	transmembrane	UniProt_topology	No	No	No	Yes	No
transmembrane	transmembrane	UniProt_keyword	No	No	No	Yes	No

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Protein Complex Composition (5)

5 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
CYP26C1 DDOST EPB42 NOS3 RHBDF2 RTN3 SACM1L SLC22A5 SURF4 SV2A TMED10 TMED7 TMED9 UBC ZMPSTE24	O15260 O75844 O76082 O95197 P0CG48 P16452 P29474 P39656 P49755 Q6PJF5 Q6V0L0 Q7L0J3 Q9BVK6 Q9NTJ5 Q9Y3B3	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5481
ATP13A1 CEPT1 LMAN2 RHBDF2 SEC23A SURF4 TMED10 TMED2 TMED4 TMED5 TMED7 TMED9 UBC	O15260 P0CG48 P49755 Q12907 Q15363 Q15436 Q6PJF5 Q7Z7H5 Q9BVK6 Q9HD20 Q9Y3A6 Q9Y3B3 Q9Y6K0	1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5483
CP DAD1 DDOST MAGT1 RHBDF2 RPN2 SEC61A2 SEC61B SEC61G SEC62 SEC63 STT3B UBC ZDHHC6	P00450 P04844 P0CG48 P39656 P60059 P60468 P61803 Q6PJF5 Q8TCJ2 Q99442 Q9H0U3 Q9H6R6 Q9H9S3 Q9UGP8	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8314
CERS6 DAD1 DDOST MAGT1 RHBDF2 RPN2 SEC61A2 SEC61B SEC61G SEC62 SEC63 STT3B TMEM258 UBC	P04844 P0CG48 P39656 P60059 P60468 P61165 P61803 Q6PJF5 Q6ZMG9 Q8TCJ2 Q99442 Q9H0U3 Q9H9S3 Q9UGP8	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8855
DAD1 DDOST RHBDF2 RPN2 SEC61A2 SEC61B SEC61G SEC62 SEC63 SPCS1 SPCS2 STT3B TRAM1 UBC	P04844 P0CG48 P39656 P60059 P60468 P61803 Q15005 Q15629 Q6PJF5 Q8TCJ2 Q99442 Q9H9S3 Q9UGP8 Q9Y6A9	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9530

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometryMass spectrometry [LTQ-FT Ultra]Mass spectrometry [QTOF]Western blotting	3	32841894 32795414 29689087

Sequence, Structure & Domains13

Sequences

Length

856

Mass

96,686

Sequence

MASADKNGGSVSSVSSSRLQSRKPPNLSITIPPPEKETQAPGEQDSMLPEGFQNRRLKKSQPRTWAAHTTACPPSFLPKRKNPAYLKSVSLQEPRSRWQESSEKRPGFRRQASLSQSIRKGAAQWFGVSGDWEGQRQQWQRRSLHHCSMRYGRLKASCQRDLELPSQEAPSFQGTESPKPCKMPKIVDPLARGRAFRHPEEMDRPHAPHPPLTPGVLSLTSFTSVRSGYSHLPRRKRMSVAHMSLQAAAALLKGRSVLDATGQRCRVVKRSFAFPSFLEEDVVDGADTFDSSFFSKEEMSSMPDDVFESPPLSASYFRGIPHSASPVSPDGVQIPLKEYGRAPVPGPRRGKRIASKVKHFAFDRKKRHYGLGVVGNWLNRSYRRSISSTVQRQLESFDSHRPYFTYWLTFVHVIITLLVICTYGIAPVGFAQHVTTQLVLRNKGVYESVKYIQQENFWVGPSSIDLIHLGAKFSPCIRKDGQIEQLVLRERDLERDSGCCVQNDHSGCIQTQRKDCSETLATFVKWQDDTGPPMDKSDLGQKRTSGAVCHQDPRTCEEPASSGAHIWPDDITKWPICTEQARSNHTGFLHMDCEIKGRPCCIGTKGSCEITTREYCEFMHGYFHEEATLCSQVHCLDKVCGLLPFLNPEVPDQFYRLWLSLFLHAGVVHCLVSVVFQMTILRDLEKLAGWHRIAIIFILSGITGNLASAIFLPYRAEVGPAGSQFGLLACLFVELFQSWPLLERPWKAFLNLSAIVLFLFICGLLPWIDNIAHIFGFLSGLLLAFAFLPYITFGTSDKYRKRALILVSLLAFAGLFAALVLWLYIYPINWPWIEHLTCFPFTSRFCEKYELDQVLH

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q6PJF5-1; Sequence=Displayed; Name=2; IsoId=Q6PJF5-2; Sequence=VSP_034368

Alternative Sequence

51..79; Missing (in isoform 2)

3D Structural Models

Turn

518..520; 528..530; 553..555; 589..591; 843..850

Helix

373..377; 388..395; 403..422; 463..469; 474..477; 481..495; 513..515; 563..565; 571..573; 613..618; 630..632; 635..640; 656..659; 660..662; 667..688; 690..711; 721..737; 739..741; 745..763; 770..787; 795..824; 832..837

Beta Strand

429..440; 442..454; 457..460; 498..504; 508..511; 521..524; 540..542; 556..558; 578..580; 598..601; 603..606; 608..611; 742..744; 852..854

3D Structure

Electron microscopy (5)

Domain & Motif Annotations

Compositional Bias

94..106; Basic and acidic residues

Region

1..115; Disordered; 165..184; Disordered; 191..271; Involved in interaction with FRMD8; 531..553; Disordered

Protein Families

Peptidase S54 family

Sequence Similarities

Belongs to the peptidase S54 family.

Clinical Relevance5

Disease Involvement (2)

Disease variantPalmoplantar keratoderma

Related Diseases (2)

Skin disease Solid tumour/cancer

Biomarker

Investigative

Drugs (4)

PAREGORIC FENTANYL CITRATE NULL OXYCODONE

Antibody

HPA018080

Supporting Publications6

PMID	Title	Abstract
34265469	Proteomic Landscape of Exosomes Reveals the Functional Contributions of CD151 in Triple-Negative Breast Cancer.	Furthermore, utilizing quantitative proteomics approach to reveal the proteomes of CD151-deleted exosomes and cells, we found that exosomal CD151 facilitated secretion of ribosomal proteins via exosomes while inhibiting exosome secretion of complement proteins. Moreover, we proved that CD151-deleted exosomes significantly decreased the migration and invasion of TNBC cells. Most importantly, we found that the tetraspanin CD151 expression levels in TNBC-derived serum exosomes were significantly higher than those exosomes from healthy subjects, and we validated our findings with samples from 16 additional donors. This is the first comparative study of the proteomes of TNBC patient-derived and CD151-deleted exosomes.
38576002	Therapy-induced senescent tumor cell-derived extracellular vesicles promote colorectal cancer progression through SERPINE1-mediated NF-κB p65 nuclear translocation.	No abstract available
39195200	Analysis of Cytotoxic Granules and Constitutively Produced Extracellular Vesicles from Large Granular Lymphocytic Leukemia Cell Lines.	No abstract available
39408670	Proteomic Characterization of Corneal Epithelial and Stromal Cell-Derived Extracellular Vesicles.	No abstract available
39948040	Quantitative proteomics identifies possible flow of metastatic cues between progressive stages of colorectal cancer via transfer of ceramide-dependent exosomal cargoes.	No abstract available
40689422	Defining the Ovarian Cancer Precancerous Landscape through Modeling Fallopian Tube Epithelium Reprogramming Driven by Extracellular Vesicles.	No abstract available