Protein detail

NFAM1

NFAT activation molecule 1 (Calcineurin/NFAT-activating ITAM-containing protein) (NFAT-activating protein with ITAM motif 1)

Entry name NFAM1	UniProt ID Q8NET5	EVMP confidence score 0.50
Supporting publications (n) 2	Transmembrane count 1	Protein classification Predicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information12

Protein Names

NFAT activation molecule 1 (Calcineurin/NFAT-activating ITAM-containing protein) (NFAT-activating protein with ITAM motif 1)

Protein Class

Predicted membrane proteins

Transmembrane

164..184; Helical

Transmembrane Count

Ensembl

ENSG00000235568

Entrez Gene Symbol

NFAM1

Gene Synonym

CNAIP

Gene Description

NFAT activating protein with ITAM motif 1

Chromosome

Position

42380407-42432403

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization4

Tissue Specificblood vesselCell SpecificSchwann cellsSingle-Nuclei Brain Specificcommitted oligodendrocyte precursor

Function & Pathway6

Cellular Component (4)

Molecular Function (2)

Biological Process (3)

Reactome (2)

Canonical Pathways

M153 Pid p75 ntr pathway

Mediation Categories (3)

Fusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence23

Enzyme-Mediated Modification (2)

2 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
NFAM1	SRC	P12931	Y	220	phosphorylation	Li2012
NFAM1	SRC	P12931	Y	231	phosphorylation	Li2012

Ligand-Receptor Signaling (18)

18 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	Yes	No
intracellular	intracellular	GO_Intercell	No	No	No	Yes	No
intracellular	intracellular	OmniPath	No	No	No	Yes	No
transmembrane	transmembrane	UniProt_location	No	No	No	Yes	No
transmembrane	transmembrane	UniProt_topology	No	No	No	Yes	No
transmembrane	transmembrane	UniProt_keyword	No	No	No	Yes	No
transmembrane	transmembrane	GO_Intercell	No	No	No	Yes	No
transmembrane	transmembrane	LOCATE	No	No	No	Yes	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	Yes	No
transmembrane	transmembrane	OmniPath	No	No	No	Yes	No

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Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	DENR DOCK11 HSPA14 MCTS1 NFAM1 OTUD6B	O43583 Q0VDF9 Q5JSL3 Q8N6M0 Q8NET5 Q9ULC4	0:0:0:0:0:0	hu.MAP2
	HSPA14 NFAM1	Q0VDF9 Q8NET5	0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometry	1	39535266

Sequence, Structure & Domains7

Sequences

Length

270

Mass

29,686

Sequence

MENQPVRWRALPGLPRPPGLPAAPWLLLGVLLLPGTLRLAGGQSVTHTGLPIMASLANTAISFSCRITYPYTPQFKVFTVSYFHEDLQGQRSPKKPTNCHPGLGTENQSHTLDCQVTLVLPGASATGTYYCSVHWPHSTVRGSGTFILVRDAGYREPPQSPQKLLLFGFTGLLSVLSVVGTALLLWNKKRMRGPGKDPTRKCPDPRSASSPKQHPSESVYTALQRRETEVYACIENEDGSSPTAKQSPLSQERPHRFEDDGELNLVYENL

Domain & Motif Annotations

Compositional Bias

194..204; Basic and acidic residues; 207..219; Polar residues; 239..250; Polar residues

Domain (CC)

The ITAM domain displays no close similarity to any existing ITAMs, except for four conserved positions. The phosphorylated ITAM domain binds ZAP70 and SYK.

Domain (FT)

50..150; Ig-like V-type; 209..237; ITAM

Region

190..219; Disordered; 234..262; Disordered

Clinical Relevance1

Antibody

HPA031812

Supporting Publications2

PMID	Title	Abstract
35119778	Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis.	No abstract available
38716512	Assessment of urine sample collection and processing variables for extracellular vesicle-based proteomics.	No abstract available