Protein detail

NFAM1

NFAT activation molecule 1 (Calcineurin/NFAT-activating ITAM-containing protein) (NFAT-activating protein with ITAM motif 1)

Entry name NFAM1	UniProt ID Q8NET5	EVMP confidence score 0.40
Supporting publications (n) 2	Transmembrane count 1	Protein classification Predicted membrane proteins

Basic Information

Protein Names

NFAT activation molecule 1 (Calcineurin/NFAT-activating ITAM-containing protein) (NFAT-activating protein with ITAM motif 1)

Protein Class

Predicted membrane proteins

Transmembrane

164..184; Helical

Transmembrane Count

Ensembl

ENSG00000235568

Entrez Gene Symbol

NFAM1

Gene Synonym

CNAIP

Gene Description

NFAT activating protein with ITAM motif 1

Chromosome

Position

42380407-42432403

Supporting publications (n)

EVMP confidence score

0.40

Fluorescence & Localization

Tissue Specificblood vesselCell SpecificSchwann cellsSingle-Nuclei Brain Specificcommitted oligodendrocyte precursor

Function & Pathway

Cellular Component (4)

Molecular Function (2)

Biological Process (3)

Reactome (2)

Canonical Pathways

M153 Pid p75 ntr pathway

Mediation Categories (3)

Fusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence23

Enzyme-Mediated Modification (2)

2 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
NFAM1	SRC	P12931	Y	220	phosphorylation	Li2012
NFAM1	SRC	P12931	Y	231	phosphorylation	Li2012

Ligand-Receptor Signaling (18)

18 records.

Category	Parent	Database	Receiver	Plasma Membrane (Transmembrane)
plasma_membrane	plasma_membrane	UniProt_location		Yes
plasma_membrane	plasma_membrane	OmniPath		Yes
plasma_membrane_transmembrane	plasma_membrane_transmembrane	Membranome		Yes
plasma_membrane_transmembrane	plasma_membrane_transmembrane	OmniPath		Yes
cell_surface	cell_surface	Surfaceome		Yes
cell_surface	cell_surface	OmniPath		Yes
receptor	receptor	CellCellInteractions	Yes	Yes
transmembrane	transmembrane_predicted	Phobius		Yes

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Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	DENR DOCK11 HSPA14 MCTS1 NFAM1 OTUD6B	O43583 Q0VDF9 Q5JSL3 Q8N6M0 Q8NET5 Q9ULC4	0:0:0:0:0:0	hu.MAP2
	HSPA14 NFAM1	Q0VDF9 Q8NET5	0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometry	1	39535266

Sequence, Structure & Domains

Sequences

Length

270

Mass

29,686

Sequence

MENQPVRWRALPGLPRPPGLPAAPWLLLGVLLLPGTLRLAGGQSVTHTGLPIMASLANTAISFSCRITYPYTPQFKVFTVSYFHEDLQGQRSPKKPTNCHPGLGTENQSHTLDCQVTLVLPGASATGTYYCSVHWPHSTVRGSGTFILVRDAGYREPPQSPQKLLLFGFTGLLSVLSVVGTALLLWNKKRMRGPGKDPTRKCPDPRSASSPKQHPSESVYTALQRRETEVYACIENEDGSSPTAKQSPLSQERPHRFEDDGELNLVYENL

Domain & Motif Annotations

Compositional Bias

194..204; Basic and acidic residues; 207..219; Polar residues; 239..250; Polar residues

Domain (CC)

The ITAM domain displays no close similarity to any existing ITAMs, except for four conserved positions. The phosphorylated ITAM domain binds ZAP70 and SYK.

Domain (FT)

50..150; Ig-like V-type; 209..237; ITAM

Region

190..219; Disordered; 234..262; Disordered

Clinical Relevance

Antibody

HPA031812

Supporting Publications2

PMID	Title	Related sentences
35119778	Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis.	No related sentences available
38716512	Assessment of urine sample collection and processing variables for extracellular vesicle-based proteomics.	No related sentences available