Protein detail

UBA3

NEDD8-activating enzyme E1 catalytic subunit (EC 6.2.1.64) (NEDD8-activating enzyme E1C) (Ubiquitin-activating enzyme E1C) (Ubiquitin-like modifier-activating enzyme 3) (Ubiquitin-activating enzyme 3)

Entry name UBA3	UniProt ID Q8TBC4	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification EnzymesEssential proteinsMetabolic proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Protein Class (4)

EnzymesEssential proteinsMetabolic proteinsPredicted intracellular proteins

Protein Function (3)

Enzymes
ENZYME proteins:Ligase
Predicted intracellular proteins

Ensembl

ENSG00000144744

Entrez Gene Symbol

UBA3

Gene Synonym (3)

hUba3NAE2UBE1C

Gene Description

Ubiquitin like modifier activating enzyme 3

Chromosome

Position

69054730-69080408

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization7

Tissue Specificparathyroid glandBrain Regional Specificchoroid plexusCell SpecificEndometrial glandular cellsSingle-Nuclei Brain Specificchoroid plexus epithelial cellBlood Cell Specificmyeloid DCBlood Lineage Specificdendritic cells

Function & Pathway7

Protein Function (3)

Enzymes
ENZYME proteins:Ligase
Predicted intracellular proteins

Cellular Component (4)

Molecular Function (6)

Biological Process (3)

KEGG

hsa04120 Ubiquitin mediated proteolysis

Reactome (15)

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Mediation Categories (3)

Clinical-translation mediationImmune mediationMetabolism mediation

Relations & Evidence24

Ligand-Receptor Signaling (4)

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Protein Complex Composition (19)

19 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
NAE	NAE1 UBA3	Q13564 Q8TBC4	2:2	SIGNORCORUMComplexPortalCompleathu.MAP2PDB	CORUM:2162PDB:1yovCompleat:HC1053intact:EBI-9020459SIGNOR:SIGNOR-C131	9545234 17220875 14755292 12646924 19352404
	ARF6 CUL1 CUL2 CUL5 DCUN1D1 NAE1 NEDD8 RBX1 RNF7 SLC25A20 UBA3 UBE2M	O43772 P61081 P62330 P62877 Q13564 Q13616 Q13617 Q15843 Q8TBC4 Q93034 Q96GG9 Q9UBF6	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6450
	ERH HSPH1 NAE1 TUSC2 UBA3 UBE2M	O75896 P61081 P84090 Q13564 Q8TBC4 Q92598	0:0:0:0:0:0	hu.MAP2
	HSPH1 NAE1 TUSC2 UBA3	O75896 Q13564 Q8TBC4 Q92598	0:0:0:0	hu.MAP2
	CUL1 CUL2 CUL3 CUL4A CUL5 DCUN1D1 DCUN1D5 NAE1 NEDD8 RBX1 RNF7 UBA3 UBC UBE2M UBXN7	O94888 P0CG48 P61081 P62877 Q13564 Q13616 Q13617 Q13618 Q13619 Q15843 Q8TBC4 Q93034 Q96GG9 Q9BTE7 Q9UBF6	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6255
	COPS6 CUL1 CUL2 CUL3 CUL4A CUL4B CUL5 DCUN1D1 NAE1 NEDD8 RBX1 RNF7 UBA3 UBE2M UBXN7	O94888 P61081 P62877 Q13564 Q13616 Q13617 Q13618 Q13619 Q13620 Q15843 Q7L5N1 Q8TBC4 Q93034 Q96GG9 Q9UBF6	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5123
	CUL1 CUL2 CUL5 DCUN1D1 G6PD NAA40 NAE1 NEDD8 RBX1 RNF7 UBA3 UBC UBE2M	P0CG48 P11413 P61081 P62877 Q13564 Q13616 Q13617 Q15843 Q86UY6 Q8TBC4 Q93034 Q96GG9 Q9UBF6	1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5750
	COPS6 CUL1 CUL2 CUL4A CUL5 DCUN1D1 GPS1 KLHDC10 NAE1 NEDD8 RBX1 RNF7 UBA3 UBC UBE2M	P0CG48 P61081 P62877 Q13098 Q13564 Q13616 Q13617 Q13619 Q15843 Q6PID8 Q7L5N1 Q8TBC4 Q93034 Q96GG9 Q9UBF6	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4017
	CUL1 CUL2 CUL5 DCUN1D1 KLHDC10 NAE1 NEDD8 RBX1 RNF7 SH3GLB1 UBA3 UBC UBE2M	P0CG48 P61081 P62877 Q13564 Q13616 Q13617 Q15843 Q6PID8 Q8TBC4 Q93034 Q96GG9 Q9UBF6 Q9Y371	1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7366
	AR UBA3	P10275 Q8TBC4	4:4	PDB	PDB:5jjm

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Size Exclusion Chromatography	Mass spectrometry	1	28211531

Sequence, Structure & Domains12

Sequences

Length

463

Mass

51,852

Sequence

MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q8TBC4-1; Sequence=Displayed; Name=2; IsoId=Q8TBC4-2; Sequence=VSP_041127

Alternative Sequence

8..21; Missing (in isoform 2)

3D Structural Models

Turn

35..38; 109..111; 215..217; 365..367; 393..395

Helix

39..46; 63..67; 80..90; 106..108; 117..119; 124..135; 148..150; 153..156; 169..181; 192..194; 221..227; 236..241; 246..255; 257..260; 275..291; 299..306; 314..333; 382..391; 419..430; 434..436; 437..440

Beta Strand

72..75; 96..100; 142..146; 160..164; 185..188; 189..191; 198..204; 207..213; 341..346; 348..350; 352..356; 372..376; 377..379; 401..406; 409..414; 431..433; 443..447; 451..453; 455..460

3D Structure

NMR spectroscopy (1); X-ray crystallography (12)

Domain & Motif Annotations

Region

53..70; Interaction with UBE2M N-terminus; 157..161; Interaction with UBE2M N-terminus; 192..217; Interaction with UBE2M N-terminus; 227..229; Interaction with NEDD8; 242..248; Interaction with NAE1; 292..295; Interaction with NAE1; 331..338; Interaction with UBE2M N-terminus; 352..357; Interaction with NEDD8; 368..463; Interaction with UBE2M core domain

Protein Families (2)

Ubiquitin-activating E1 family
UBA3 subfamily

Sequence Similarities

Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.

Clinical Relevance5

Drugs

PEVONEDISTAT

Antibody (3)

HPA034873 HPA034874 HPA065335

Interaction Protein (3)

ENSG00000129559ENSG00000130725ENSG00000159593

Interaction Count

Interaction Dataset (2)

intact_biogridintact_biogrid_opencell

Supporting Publications1

PMID	Title	Abstract
38731868	The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer.	No abstract available