Protein detail

UBA3

NEDD8-activating enzyme E1 catalytic subunit (EC 6.2.1.64) (NEDD8-activating enzyme E1C) (Ubiquitin-activating enzyme E1C) (Ubiquitin-like modifier-activating enzyme 3) (Ubiquitin-activating enzyme 3)

Entry name UBA3	UniProt ID Q8TBC4	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification EnzymesEssential proteinsMetabolic proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Protein Class (4)

EnzymesEssential proteinsMetabolic proteinsPredicted intracellular proteins

Protein Function (3)

Enzymes
ENZYME proteins:Ligase
Predicted intracellular proteins

Ensembl

ENSG00000144744

Entrez Gene Symbol

UBA3

Gene Synonym (3)

hUba3NAE2UBE1C

Gene Description

Ubiquitin like modifier activating enzyme 3

Chromosome

Position

69054730-69080408

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization7

Tissue Specificparathyroid glandBrain Regional Specificchoroid plexusCell SpecificEndometrial glandular cellsSingle-Nuclei Brain Specificchoroid plexus epithelial cellBlood Cell Specificmyeloid DCBlood Lineage Specificdendritic cells

Function & Pathway7

Protein Function (3)

Enzymes
ENZYME proteins:Ligase
Predicted intracellular proteins

Cellular Component (4)

Molecular Function (6)

Biological Process (3)

KEGG

hsa04120 Ubiquitin mediated proteolysis

Reactome (15)

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Mediation Categories (3)

Clinical-translation mediationImmune mediationMetabolism mediation

Relations & Evidence24

Ligand-Receptor Signaling (4)

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Protein Complex Composition (19)

19 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ARF6 CUL1 CUL2 CUL5 DCUN1D1 DSP NAE1 NEDD8 RBX1 RNF7 SLC25A15 UBA3 UBE2M	P15924 P61081 P62330 P62877 Q13564 Q13616 Q13617 Q15843 Q8TBC4 Q93034 Q96GG9 Q9UBF6 Q9Y619	1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5767
CUL1 CUL2 CUL3 CUL5 DCUN1D1 NAE1 NEDD8 RBX1 RNF7 UBA3 UBE2F UBE2M	P61081 P62877 Q13564 Q13616 Q13617 Q13618 Q15843 Q8TBC4 Q93034 Q969M7 Q96GG9 Q9UBF6	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8351
CUL1 CUL2 CUL5 DCUN1D1 ILKAP NAE1 NEDD8 RBX1 RNF7 SH3GLB1 UBA3 UBE2M	P61081 P62877 Q13564 Q13616 Q13617 Q15843 Q8TBC4 Q93034 Q96GG9 Q9H0C8 Q9UBF6 Q9Y371	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7164
KCTD6 KLHL42 NAE1 UBA3 UBE2M	P61081 Q13564 Q8NC69 Q8TBC4 Q9P2K6	0:0:0:0:0	hu.MAP2
NAE1 UBA3 UBE2M	P61081 Q13564 Q8TBC4	2:2:2	PDB	PDB:1tt5
UBA3 UBE2M	P61081 Q8TBC4	1:1	PDB	PDB:1y8x
NAE1 NEDD8 UBA3	Q13564 Q15843 Q8TBC4	4:4:4	PDB	PDB:3gznPDB:1r4nPDB:1r4mPDB:3dbhPDB:3dblPDB:3dbr
NAE1 SKIC8 UBA3 USH1C	Q13564 Q8TBC4 Q9GZS3 Q9Y6N9	0:0:0:0	Havugimana2012	Havugimana2012:C_229
UBA3 UBE2F	Q8TBC4 Q969M7	1:1	PDB	PDB:3fn1

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Size Exclusion Chromatography	Mass spectrometry	1	28211531

Sequence, Structure & Domains12

Sequences

Length

463

Mass

51,852

Sequence

MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q8TBC4-1; Sequence=Displayed; Name=2; IsoId=Q8TBC4-2; Sequence=VSP_041127

Alternative Sequence

8..21; Missing (in isoform 2)

3D Structural Models

Turn

35..38; 109..111; 215..217; 365..367; 393..395

Helix

39..46; 63..67; 80..90; 106..108; 117..119; 124..135; 148..150; 153..156; 169..181; 192..194; 221..227; 236..241; 246..255; 257..260; 275..291; 299..306; 314..333; 382..391; 419..430; 434..436; 437..440

Beta Strand

72..75; 96..100; 142..146; 160..164; 185..188; 189..191; 198..204; 207..213; 341..346; 348..350; 352..356; 372..376; 377..379; 401..406; 409..414; 431..433; 443..447; 451..453; 455..460

3D Structure

NMR spectroscopy (1); X-ray crystallography (12)

Domain & Motif Annotations

Region

53..70; Interaction with UBE2M N-terminus; 157..161; Interaction with UBE2M N-terminus; 192..217; Interaction with UBE2M N-terminus; 227..229; Interaction with NEDD8; 242..248; Interaction with NAE1; 292..295; Interaction with NAE1; 331..338; Interaction with UBE2M N-terminus; 352..357; Interaction with NEDD8; 368..463; Interaction with UBE2M core domain

Protein Families (2)

Ubiquitin-activating E1 family
UBA3 subfamily

Sequence Similarities

Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.

Clinical Relevance5

Drugs

PEVONEDISTAT

Antibody (3)

HPA034873 HPA034874 HPA065335

Interaction Protein (3)

ENSG00000129559ENSG00000130725ENSG00000159593

Interaction Count

Interaction Dataset (2)

intact_biogridintact_biogrid_opencell

Supporting Publications1

PMID	Title	Abstract
38731868	The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer.	No abstract available