EV MemProt Atlas

Basic Information11

Protein Names

Symplekin

Protein Class (3)

Essential proteinsPlasma proteinsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000125755

Entrez Gene Symbol

SYMPK

Gene Synonym (3)

Pta1SPKSYM

Gene Description

Symplekin scaffold protein

Chromosome

19

Position

45815410-45863194

Supporting publications (n)

1

EVMP confidence score

0.38

Fluorescence & Localization6

Tissue Specificbone marrowCell SpecificcDCSingle-Nuclei Brain Specificcentral nervous system macrophageBlood Cell Specificclassical monocyteBlood Lineage Specificgranulocytes

Function & Pathway8

Protein Function

Predicted intracellular proteins

Cellular Component (9)

Molecular Function

GO:0005515 protein binding

Biological Process (3)

KEGG (2)

Reactome (14)

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Canonical Pathways (3)

M5883 Naba secreted factors
M5885 Naba matrisome associated
M5889 Naba matrisome

Mediation Categories (2)

Clinical-translation mediationFusion and delivery mediation

Relations & Evidence25

Enzyme-Mediated Modification (2)

2 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
SYMPK	MAPK3	P27361	T	1,257	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
SYMPK	MAPK8	P45983	S	1,243	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (11)

11 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

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Regulatory Interaction Network (1)

1 record.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
MK03	P27361	SYMPK	Q92797	Yes	No	No	PhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:28630428

Protein Complex Composition (10)

10 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
Histone pre-RNA core cleavage complex	CPSF2 CPSF3 CSTF2 SYMPK	P33240 Q92797 Q9P2I0 Q9UKF6	1:1:1:1	ComplexPortal	intact:EBI-9023470	23071092 14755292 32029631 34230059
POL2R-SYMPK-SSU72 complex	POLR2A SSU72 SYMPK	P24928 Q92797 Q9NP77	0:0:0	CORUM	CORUM:6218	20861839
Polyadenylation complex (CSTF1CSTF2CSTF3SYMPK CPSF1CPSF2CPSF3)	CPSF1 CPSF2 CPSF3 CSTF1 CSTF2 CSTF3 SYMPK	P33240 Q05048 Q10570 Q12996 Q92797 Q9P2I0 Q9UKF6	1:1:1:1:1:1:1	CompleatCORUM	CORUM:1147Compleat:HC3064	10669729
pre-mRNA cleavage and polyadenylation specificity factor complex	CPSF1 CPSF2 CPSF3 CPSF4 FIP1L1 SYMPK WDR33	O95639 Q10570 Q6UN15 Q92797 Q9C0J8 Q9P2I0 Q9UKF6	1:1:1:1:1:1:1	ComplexPortalhu.MAPhu.MAP2	PDB:6fuwPDB:6BM0PDB:6fbsPDB:7k95PDB:6f9nPDB:6BLYintact:EBI-9023581	35177536 14755292 29358758 29208711 33122294
	CPSF1 CPSF2 CPSF3 CPSF4 FIP1L1 SYMPK	O95639 Q10570 Q6UN15 Q92797 Q9P2I0 Q9UKF6	0:0:0:0:0:0	hu.MAP
	CPSF2 CPSF3 LSM10 LSM11 SNRPB SNRPD3 SNRPE SNRPF SNRPG SYMPK	P14678 P62304 P62306 P62308 P62318 P83369 Q92797 Q969L4 Q9P2I0 Q9UKF6	1:1:1:1:1:1:1:1:1:1	PDB	PDB:6v4x
	AHCYL2 ANP32A PTS SYMPK TPI1	P39687 P60174 Q03393 Q92797 Q96HN2	1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8462
	CHD3 SYMPK	Q12873 Q92797	0:0	hu.MAP2
	CHD5 SYMPK	Q8TDI0 Q92797	0:0	hu.MAP2
	SSU72 SYMPK	Q92797 Q9NP77	2:2	PDB	PDB:4h3hPDB:3o2sPDB:3o2qPDB:4h3k

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	2	27487081 30915084

Sequence, Structure & Domains15

Sequences

Length

1,274

Mass

141,148

Sequence

MASGSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEIPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASLEFQAQITTLLVDLGTPQAEIARNMPSSKDTRKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKTESVLIKRRLSAQGQAISVVGSLSSMSPLEEEAPQAKRRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEAMKLGAVKRILRAEKAVACSGAAQVRIKILASLVTQFNSGLKAEVLSFILEDVRARLDLAFAWLYQEYNAYLAAGASGSLDKYEDCLIRLLSGLQEKPDQKDGIFTKVVLEAPLITESALEVVRKYCEDESRTYLGMSTLRDLIFKRPSRQFQYLHVLLDLSSHEKDKVRSQALLFIKRMYEKEQLREYVEKFALNYLQLLVHPNPPSVLFGADKDTEVAAPWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPELLLLVENCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPKLIKLNPIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSVKCDMKSIIKATNLCFAERNVYTSEVLAVVMQQLMEQSPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIMKQVWKYPKVWEGFIKCCQRTKPQSFQVILQLPPQQLGAVFDKCPELREPLLAHVRSFTPHQQAHIPNSIMTILEASGKQEPEAKEAPAGPLEEDDLEPLTLAPAPAPRPPQDLIGLRLAQEKALKRQLEEEQKLKPGGVGAPSSSSPSPSPSARPGPPPSEEAMDFREEGPECETPGIFISMDDDSGLTEAALLDSSLEGPLPKETAAGGLTLKEERSPQTLAPVGEDAMKTPSPAAEDAREPEAKGNS

Alternative Products

Event=Alternative splicing; Named isoforms=2; Comment=Additional isoforms seem to exist.; Name=1; IsoId=Q92797-1; Sequence=Displayed; Name=2; IsoId=Q92797-2; Sequence=VSP_014842, VSP_014843

Alternative Sequence

637..672; GSLDKYEDCLIRLLSGLQEKPDQKDGIFTKVVLEAP -> PRLCWRRHSSQRVPWRWSASTARMRVAPIWACPHFET (in isoform 2); 673..1274; Missing (in isoform 2)

3D Structural Models

Helix

32..45; 50..63; 67..73; 74..78; 79..82; 86..102; 104..106; 107..118; 123..146; 152..170; 171..173; 177..193; 205..207; 213..215; 225..242; 250..266; 268..270; 271..283; 287..289; 291..293; 294..309; 312..317; 318..327; 332..337; 342..345

Beta Strand

221..223

3D Structure

Electron microscopy (1); X-ray crystallography (7)

Domain & Motif Annotations

Compositional Bias

349..362; Basic and acidic residues; 380..392; Polar residues; 1149..1159; Basic and acidic residues; 1173..1185; Pro residues; 1263..1274; Basic and acidic residues

Repeat

31..64; HEAT 1; 67..101; HEAT 2; 104..146; HEAT 3; 153..192; HEAT 4; 227..266; HEAT 5

Motif

345..360; Nuclear localization signal

Domain (CC)

The HEAT repeats have been determined based on 3D-structure analysis of the D.melanogaster ortholog and are not detected by sequence-based prediction programs.

Region

1..124; Interaction with HSF1; 335..392; Disordered; 1102..1137; Disordered; 1149..1274; Disordered

Protein Families

Symplekin family

Sequence Similarities

Belongs to the Symplekin family.

Clinical Relevance4

Antibody (2)

HPA041756 HPA042449

Interaction Protein (6)

ENSG00000071894ENSG00000101811ENSG00000111605ENSG00000165934ENSG00000177613ENSG00000198900

Interaction Count

6

Interaction Dataset (3)

intact_biogridbiogrid_opencellbiogrid_bioplex

Supporting Publications1

PMID	Title	Abstract
37980468	Lymphocyte migration regulation related proteins in urine exosomes may serve as a potential biomarker for lung cancer diagnosis.	Lung cancer patients had reduced expression of WASL and increased expression of STK10 and WNK1 proteins in urine exosomes compared to normal people.

Entry name SYMPK	UniProt ID Q92797	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification Essential proteinsPlasma proteinsPredicted intracellular proteins