Protein detail

EHD4

EH domain-containing protein 4 (Hepatocellular carcinoma-associated protein 10/11) (PAST homolog 4)

Entry name
EHD4
UniProt ID
EVMP confidence score
0.88
Supporting publications (n)
33
Transmembrane count
Protein classification
Plasma proteinsPredicted intracellular proteins
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information11
Protein Names
EH domain-containing protein 4 (Hepatocellular carcinoma-associated protein 10/11) (PAST homolog 4)
Protein Class (2)
Plasma proteinsPredicted intracellular proteins
Protein Function
Predicted intracellular proteins
Entrez Gene Symbol
Gene Synonym
PAST4
Gene Description
EH domain containing 4
Chromosome
15
Position
41895933-41972557
Supporting publications (n)
33
EVMP confidence score
0.88
Fluorescence & Localization8
Tissue Specificbone marrowBrain Regional Specificcerebral cortexCell SpecificEsophageal apical cellsSingle-Nuclei Brain SpecificleukocyteBlood Cell Specificclassical monocyteBlood Lineage SpecificgranulocytesSecretome LocationSecreted to bloodSecretome FunctionImmunity
Function & Pathway6
Relations & Evidence12

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
EHD4EGFRP00533Y456phosphorylationKEAKEA:17570479

Ligand-Receptor Signaling (8)

8 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
extracellularextracellularLOCATENoNoNoNoNo
extracellularextracellularOmniPathNoNoNoNoNo
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo
plasma_membraneplasma_membraneUniProt_locationNoNoNoNoNo
plasma_membraneplasma_membraneOmniPathNoNoNoNoNo

Protein Complex Composition (2)

2 records.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
CUL4AEHD4GSTK1TSC22D1Q13619Q15714Q9H223Q9Y2Q31:1:1:1CompleatCFinderCompleat:HC5094
EHD4RBSNQ9H1K0Q9H2230:0hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Size Exclusion ChromatographyR Sequencing137740815
Sequence, Structure & Domains8

Sequences

Length
541
Mass
61,175
Sequence
MFSWMGRQAGGRERAGGADAVQTVTGGLRSLYLRKVLPLEEAYRFHEFHSPALEDADFENKPMILLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMYGETEGSTPGNALVVDPKKPFRKLSRFGNAFLNRFMCSQLPNQVLKSISVIDSPGILSGEKQRISRGYDFCQVLQWFAERVDRIILLFDAHKLDISDEFSEAIKAFRGQDDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVINTPEVLRVYIGSFWAQPLQNTDNRRLFEAEAQDLFRDIQSLPQKAAVRKLNDLIKRARLAKVHAYIISYLKKEMPSVFGKENKKRELISRLPEIYIQLQREYQISAGDFPEVKAMQEQLENYDFTKFHSLKPKLIEAVDNMLSNKISPLMNLISQEETSTPTQLVQGGAFDGTTEGPFNQGYGEGAKEGADEEEWVVAKDKPVYDELFYTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKLADCDCDGMLDEEEFALAKHLIKIKLDGYELPSSLPPHLVPPSHRKSLPKAD

Domain & Motif Annotations

Domain (CC)
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
Domain (FT)
58..289; Dynamin-type G; 447..535; EH; 479..514; EF-hand
Region
68..75; G1 motif; 94..95; G2 motif; 156..159; G3 motif; 222..225; G4 motif; 246; G5 motif
Protein Families (3)
  • TRAFAC class dynamin-like GTPase superfamily
  • Dynamin/Fzo/YdjA family
  • EHD subfamily
Sequence Similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
Clinical Relevance1
Supporting Publications33
PMIDTitleAbstract
38014595Proteome and immune responses of extracellular vesicles derived from macrophages infected with the periodontal pathogen Tannerella forsythia.No abstract available
38113368In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.No abstract available
38207106Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.No abstract available
38607062Enrichment, Characterization, and Proteomic Profiling of Small Extracellular Vesicles Derived from Human Limbal Mesenchymal Stromal Cells and Melanocytes.No abstract available
38891077Altered Extracellular Vesicle-Derived Protein and microRNA Signatures in Bronchoalveolar Lavage Fluid from Patients with Chronic Obstructive Pulmonary Disease.No abstract available
39001700Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles.No abstract available
39207047The trajectory of vesicular proteomic signatures from HBV-HCC by chitosan-magnetic bead-based separation and DIA-proteomic analysis.No abstract available
40189497Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.No abstract available
40465195Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome.No abstract available
40840701Multiomics analysis to evaluate the enrichment of extracellular vesicles from human plasma.No abstract available
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