Protein detail

S12A5

Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (hKCC2) (Neuronal K-Cl cotransporter)

Protein symbol S12A5	UniProt ID Q9H2X9	EVMP score 0.25
Frequency 1	Transmembrane count 12	Protein classification Disease related genesFDA approved drug targetsHuman disease related genesMetabolic proteinsPredicted intracellular proteinsPredicted membrane proteinsTransporters

Basic Information

Protein Names

Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (hKCC2) (Neuronal K-Cl cotransporter)

Protein Class

Disease related genesFDA approved drug targetsHuman disease related genesMetabolic proteinsPredicted intracellular proteinsPredicted membrane proteinsTransporters

Protein Function

Predicted intracellular proteins
Human disease related genes:Nervous system diseases:Epilepsy
Transporters:Electrochemical Potential-driven transporters
Disease related genes
FDA approved drug targets:Small molecule drugs

Transmembrane

99..120; Discontinuously helical; Name=1; 130..151; Helical; Name=2; 175..203; Helical; Name=3; 230..250; Helical; Name=4; 251..276; Helical; Name=5; 403..420; Helical; Name=6; 430..453; Helical; Name=7; 486..513; Helical; Name=8; 535..555; Helical; Name=9; 556..578; Helical; Name=10; 593..615; Helical; Name=11; 616..632; Helical; Name=12

Transmembrane Count

Ensembl

ENSG00000124140

Entrez Gene Symbol

SLC12A5

Gene Synonym

KCC2KIAA1176

Gene Description

Solute carrier family 12 member 5

Chromosome

Position

46021690-46060150

Frequency

EVMP Score

0.25

Fluorescence & Localization

Cell SpecificAstrocytesSingle-Nuclei Brain Specificendothelial cellBlood Cell Specificneutrophil

Function & Pathway

Protein Function

Predicted intracellular proteins
Human disease related genes:Nervous system diseases:Epilepsy
Transporters:Electrochemical Potential-driven transporters
Disease related genes
FDA approved drug targets:Small molecule drugs

Cellular Component

Molecular Function

Biological Process

KEGG

hsa04727 GABAergic synapse

Reactome

Mediation Categories

Clinical-translation mediationFusion and delivery mediation

Relations & Evidence

Enzyme-Mediated Modification

13 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
SLC12A5	STK39	Q9UEW8	T	6	phosphorylation	PhosphoSite_ProtMapperProtMapper
SLC12A5	STK39	Q9UEW8	T	1,032	phosphorylation	PhosphoSite_ProtMapperProtMapper
SLC12A5	STK39	Q9UEW8	T	1,030	phosphorylation	REACH_ProtMapperSparser_ProtMapperProtMapper	ProtMapper:24567703 ProtMapper:29092909 ProtMapper:26142773
SLC12A5	STK39	Q9UEW8	T	929	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:24567703
SLC12A5	WNK3	Q9BYP7	T	1,030	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:24139641
SLC12A5	WNK3	Q9BYP7	T	929	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:24139641
SLC12A5	OXSR1	O95747	T	1,030	phosphorylation	REACH_ProtMapperSparser_ProtMapperProtMapper	ProtMapper:24567703 ProtMapper:26142773
SLC12A5	OXSR1	O95747	T	929	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:24567703
SLC12A5	WNK1	Q9H4A3	T	1,030	phosphorylation	RLIMS-P_ProtMapperREACH_ProtMapperProtMapper	ProtMapper:27025876 ProtMapper:29176664
SLC12A5	WNK1	Q9H4A3	T	929	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:27025876

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Ligand-Receptor Signaling

23 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
extracellular	extracellular	OmniPath	No	No	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
transporter	transporter	Surfaceome	No	Yes	No	No	No
solute_carrier	transporter	Almen2009	No	Yes	No	No	No
apc	transporter	Surfaceome	No	Yes	No	No	No
slc	transporter	Surfaceome	No	Yes	No	No	No
slc12	transporter	Surfaceome	No	Yes	No	No	No
transporter	transporter	OmniPath	No	Yes	No	No	No

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Regulatory Interaction Network

1 record.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
WNK3	Q9BYP7	S12A5	Q9H2X9	Yes	No	Yes	REACH_ProtMapperSIGNORProtMapper	ProtMapper:24139641 SIGNOR:21613606

Protein Complex Composition

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	DCK DGUOK ERC2 SLC12A5	O15083 P27707 Q16854 Q9H2X9	0:0:0:0	hu.MAP
	SLC12A5	Q9H2X9	2	PDB	PDB:7d8zPDB:6m23

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	2	27487081 35763629

Sequence, Structure & Domains

Sequences

Length

1,139

Mass

126,184

Sequence

MSRRFTVTSLPPAGPARSPDPESRRHSVADPRHLPGEDVKGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; Synonyms=KCC2a; IsoId=Q9H2X9-1; Sequence=Displayed; Name=2; Synonyms=KCC2b; IsoId=Q9H2X9-2; Sequence=VSP_029909

Alternative Sequence

1..40; MSRRFTVTSLPPAGPARSPDPESRRHSVADPRHLPGEDVK -> MLNNLTDCEDGDGGANP (in isoform 2)

3D Structural Models

Turn

152..154; 156..162; 192..194; 300..302; 371..373; 477..479; 489..495; 521..524; 572..574; 577..579; 618..620; 757..760; 808..810; 904..906; 923..927; 946..948; 953..957; 1107..1110; 1118..1120

Helix

82..88; 99..102; 103..105; 122..125; 127..133; 137..141; 143..149; 163..177; 188..191; 198..201; 203..228; 245..270; 272..277; 279..299; 341..344; 356..359; 378..380; 423..430; 431..433; 437..441; 450..463; 466..476; 482..485; 500..502; 508..520; 525..538; 543..549; 559..567; 569..571; 581..605; 621..634; 638..644; 646..663; 667..681; 713..722; 738..741; 743..756; 773..782; 812..823; 834..836; 859..868; 894..903; 931..940; 1078..1087; 1104..1106; 1111..1117

Beta Strand

241..243; 308..314; 326..328; 332..334; 348..352; 364..369; 395..397; 412..414; 442..446; 503..505; 555..558; 614..616; 664..666; 685..687; 688..691; 698..700; 729..736; 763..772; 793..796; 803..806; 827..832; 850..852; 854..856; 871..876; 880..885; 887..889; 910..915; 949..951; 1088..1091; 1093..1096; 1122..1128

3D Structure

Electron microscopy (2)

Domain & Motif Annotations

Compositional Bias

19..45; Basic and acidic residues; 46..55; Polar residues; 98..111; Basic and acidic residues; 945..962; Basic and acidic residues; 982..994; Acidic residues; 1003..1012; Low complexity; 1023..1042; Basic and acidic residues

Region

1..63; Disordered; 92..116; Disordered; 667..681; Scissor helix; 942..1052; Disordered

Protein Families

SLC12A transporter family
K/Cl co-transporter subfamily

Sequence Similarities

Belongs to the SLC12A transporter family. K/Cl co-transporter subfamily.

Clinical Relevance

Disease Involvement

Disease variantEpilepsyFDA approved drug targets

Related Diseases

Epilepsy/seizure

Biomarker

Investigative

Drug Targets

FDA approved drug targets

Drugs

BUMETANIDE

Antibody

HPA072058