Protein detail

MFSD1

Lysosomal dipeptide transporter MFSD1 (Major facilitator superfamily domain-containing protein 1) (Smooth muscle cell-associated protein 4) (SMAP-4)

Entry name MFSD1	UniProt ID Q9H3U5	EVMP confidence score 0.72
Supporting publications (n) 13	Transmembrane count 12	Protein classification Predicted intracellular proteinsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Lysosomal dipeptide transporter MFSD1 (Major facilitator superfamily domain-containing protein 1) (Smooth muscle cell-associated protein 4) (SMAP-4)

Protein Class (2)

Predicted intracellular proteinsPredicted membrane proteins

Protein Function

Predicted intracellular proteins

Transmembrane

39..59; Helical; 83..103; Helical; 113..133; Helical; 135..155; Helical; 170..191; Helical; 213..233; Helical; 266..286; Helical; 303..323; Helical; 331..351; Helical; 361..381; Helical; 392..412; Helical; 418..438; Helical

Transmembrane Count

Ensembl

ENSG00000118855

Entrez Gene Symbol

MFSD1

Gene Synonym (3)

FLJ14153MinervaUG0581B09

Gene Description

Major facilitator superfamily domain containing 1

Chromosome

Position

158732198-158829719

Supporting publications (n)

EVMP confidence score

0.72

Fluorescence & Localization3

Tissue Specificparathyroid glandCell SpecificBergmann glia

Function & Pathway6

Protein Function

Predicted intracellular proteins

Cellular Component (2)

Molecular Function (2)

Biological Process (3)

Canonical Pathways (2)

M247 Pid insulin glucose pathway
M236 Pid delta np63 pathway

Mediation Categories

Fusion and delivery mediation

Relations & Evidence15

Ligand-Receptor Signaling (10)

10 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
transmembrane	transmembrane	UniProt_location	No	No	No	No	No
transmembrane	transmembrane	UniProt_topology	No	No	No	No	No
transmembrane	transmembrane	UniProt_keyword	No	No	No	No	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No
transmembrane	transmembrane_predicted	Phobius	No	No	No	No	No

Protein Complex Composition (4)

4 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ACTL8 ISYNA1 MFSD10 PGM1 RRM1 RRM2 RRM2B UBC VAMP1	P0CG48 P23763 P23921 P31350 P36871 Q14728 Q7LG56 Q9H568 Q9NPH2	1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9248
ABCE1 MFSD1 RNASEL UBC	P0CG48 P61221 Q05823 Q9H3U5	1:1:1:1	NetworkBlastCompleat	Compleat:HC4681
FUBP1 MAP2K6 MAPK14 MAPKAPK3 MFSD1 RAB10	P52564 P61026 Q16539 Q16644 Q96AE4 Q9H3U5	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8426
MAPK14 MAPKAPK3 MFSD1 RAB10 SNRPG	P61026 P62308 Q16539 Q16644 Q9H3U5	1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4288

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	34889606

Sequence, Structure & Domains10

Sequences

Length

465

Mass

51,209

Sequence

MEEEDEEARALLAGGPDEADRGAPAAPGALPALCDPSRLAHRLLVLLLMCFLGFGSYFCYDNPAALQTQVKRDMQVNTTKFMLLYAWYSWPNVVLCFFGGFLIDRVFGIRWGTIIFSCFVCIGQVVFALGGIFNAFWLMEFGRFVFGIGGESLAVAQNTYAVSWFKGKELNLVFGLQLSMARIGSTVNMNLMGWLYSKIEALLGSAGHTTLGITLMIGGITCILSLICALALAYLDQRAERILHKEQGKTGEVIKLTDVKDFSLPLWLIFIICVCYYVAVFPFIGLGKVFFTEKFGFSSQAASAINSVVYVISAPMSPVFGLLVDKTGKNIIWVLCAVAATLVSHMMLAFTMWNPWIAMCLLGLSYSLLACALWPMVAFVVPEHQLGTAYGFMQSIQNLGLAIISIIAGMILDSRGYLFLEVFFIACVSLSLLSVVLLYLVNRAQGGNLNYSARQREEIKFSHTE

Alternative Products

Event=Alternative splicing; Named isoforms=6; Name=1; IsoId=Q9H3U5-1; Sequence=Displayed; Name=2; IsoId=Q9H3U5-2; Sequence=VSP_022537, VSP_022538; Name=3; IsoId=Q9H3U5-3; Sequence=VSP_037579; Name=4; IsoId=Q9H3U5-4; Sequence=VSP_037578; Name=5; IsoId=Q9H3U5-5; Sequence=VSP_047667, VSP_047668; Name=6; IsoId=Q9H3U5-6; Sequence=VSP_047667

Alternative Sequence

1..73; Missing (in isoform 4); 1; M -> MGVALRDLPGRHVSSRSHVTAVLTVFHGRCFLPGFGVVTTFPSPSPAGAM (in isoform 5 and isoform 6); 55..110; GSYFCYDNPAALQTQVKRDMQVNTTKFMLLYAWYSWPNVVLCFFGGFLIDRVFGIR -> AIFAMIILLPFRLKLDE (in isoform 3); 55..110; GSYFCYDNPAALQTQVKRDMQVNTTKFMLLYAWYSWPNVVLCFFGGFLIDRVFGIR -> AIFAMIILLPFRLKLNE (in isoform 5); 73..78; DMQVNT -> MGHNHF (in isoform 2); 79..465; Missing (in isoform 2)

Domain & Motif Annotations

Motif

11..12; Dileucine internalization motif

Domain (CC)

The dileucine internalization motif is required for lysosomal localization.

Region

1..23; Disordered

Protein Families

Major facilitator superfamily

Sequence Similarities

Belongs to the major facilitator superfamily.

Clinical Relevance1

Antibody

HPA065858

Supporting Publications13

PMID	Title	Abstract
38168906	Defining the relationship between cellular and extracellular vesicle (EV) content in breast cancer via an integrative multi-omic analysis.	No abstract available
38321535	Identification of specific markers for human pluripotent stem cell-derived small extracellular vesicles.	No abstract available
39409015	SILAC-Based Characterization of Plasma-Derived Extracellular Vesicles in Patients Undergoing Partial Hepatectomy.	No abstract available

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