Protein detail

EHD1

EH domain-containing protein 1 (PAST homolog 1) (hPAST1) (Testilin)

Entry name EHD1	UniProt ID Q9H4M9	EVMP confidence score 0.88
Supporting publications (n) 114	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

EH domain-containing protein 1 (PAST homolog 1) (hPAST1) (Testilin)

Protein Class (3)

Plasma proteinsPredicted intracellular proteinsTransporters

Protein Function (2)

Transporters:Transporter channels and pores
Predicted intracellular proteins

Ensembl

ENSG00000110047

Entrez Gene Symbol

EHD1

Gene Synonym (5)

FLJ42622FLJ44618H-PASTHPAST1PAST1

Gene Description

EH domain containing 1

Chromosome

Position

64851642-64888296

Supporting publications (n)

114

EVMP confidence score

0.88

Fluorescence & Localization2

Cell SpecificAstrocytes

Function & Pathway6

Protein Function (2)

Transporters:Transporter channels and pores
Predicted intracellular proteins

Cellular Component (15)

Page 1 of 2

Molecular Function (7)

KEGG

hsa04144 Endocytosis

Reactome (2)

Mediation Categories (2)

Fusion and delivery mediationImmune mediation

Relations & Evidence11

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
EHD1	EGFR	P00533	Y	453	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (6)

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Protein Complex Composition (3)

3 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ARL8B CCT6A CCT8 CLTC CYP24A1 EHD1 GFPT2 GK IPO9 MTARC2 PPP2R1A QNG1 RPN1 SLC25A11 SLC27A4	O94808 P04843 P30153 P32189 P40227 P50990 Q00610 Q02978 Q07973 Q5T6V5 Q6P1M0 Q969Z3 Q96P70 Q9H4M9 Q9NVJ2	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4692
ANXA6 EHD1 PAFAH1B2 PAFAH1B3 SMAD1 TBCB	P08133 P68402 Q15102 Q15797 Q99426 Q9H4M9	0:0:0:0:0:0	hu.MAP2
ACTMAP EHD1	Q5BKX5 Q9H4M9	0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryWestern blotting	2	37886648 38343172

Sequence, Structure & Domains13

Sequences

Length

534

Mass

60,627

Sequence

MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE

3D Structural Models

Turn

523..525

Helix

440..443; 445..454; 464..473; 478..488; 498..512; 528..530

Beta Strand

458..463; 494..497

3D Structure

NMR spectroscopy (5)

Domain & Motif Annotations

Coiled Coil

198..227

Domain (CC)

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.

Domain (FT)

55..286; Dynamin-type G; 444..532; EH; 476..511; EF-hand

Region

65..72; G1 motif; 91..92; G2 motif; 153..156; G3 motif; 219..222; G4 motif; 243; G5 motif

Protein Families (3)

TRAFAC class dynamin-like GTPase superfamily
Dynamin/Fzo/YdjA family
EHD subfamily

Sequence Similarities

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.

Clinical Relevance4

Antibody (4)

HPA049890 HPA049986 HPA066751 HPA067747

Interaction Protein (5)

ENSG00000013016ENSG00000099940ENSG00000100139ENSG00000131381ENSG00000185722

Interaction Count

Interaction Dataset (2)

intact_biogridintact_biogrid_bioplex

Supporting Publications113

PMID	Title	Abstract
27320496	Exosomes in the nose induce immune cell trafficking and harbour an altered protein cargo in chronic airway inflammation.	No abstract available
27605433	Secreted primary human malignant mesothelioma exosome signature reflects oncogenic cargo.	No abstract available
27723984	Proteomic and Bioinformatic Characterization of Extracellular Vesicles Released from Human Macrophages upon Influenza A Virus Infection.	No abstract available
28054239	The Protein Content of Extracellular Vesicles Derived from Expanded Human Umbilical Cord Blood-Derived CD133+ and Human Bone Marrow-Derived Mesenchymal Stem Cells Partially Explains Why both Sources are Advantageous for Regenerative Medicine.	No abstract available
28242843	Label-free Proteomic Analysis of Exosomes Derived from Inducible Hepatitis B Virus-Replicating HepAD38 Cell Line.	No abstract available
28369848	End stage renal disease-induced hypercalcemia may promote aortic valve calcification via Annexin VI enrichment of valve interstitial cell derived-matrix vesicles.	No abstract available
28396511	Database-augmented Mass Spectrometry Analysis of Exosomes Identifies Claudin 3 as a Putative Prostate Cancer Biomarker.	No abstract available
28562335	Urinary exosomes reveal protein signatures in hypertensive patients with albuminuria.	No abstract available
28963552	Radiation alters the cargo of exosomes released from squamous head and neck cancer cells to promote migration of recipient cells.	No abstract available
29242380	Insights into the Proteome of Gastrointestinal Stromal Tumors-Derived Exosomes Reveals New Potential Diagnostic Biomarkers.	No abstract available

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