Protein detail

EHD1

EH domain-containing protein 1 (PAST homolog 1) (hPAST1) (Testilin)

Entry name
EHD1
UniProt ID
EVMP confidence score
0.88
Supporting publications (n)
114
Transmembrane count
Protein classification
Plasma proteinsPredicted intracellular proteinsTransporters
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information11
Protein Names
EH domain-containing protein 1 (PAST homolog 1) (hPAST1) (Testilin)
Protein Class (3)
Plasma proteinsPredicted intracellular proteinsTransporters
Protein Function (2)
  • Transporters:Transporter channels and pores
  • Predicted intracellular proteins
Entrez Gene Symbol
Gene Synonym (5)
FLJ42622FLJ44618H-PASTHPAST1PAST1
Gene Description
EH domain containing 1
Chromosome
11
Position
64851642-64888296
Supporting publications (n)
114
EVMP confidence score
0.88
Fluorescence & Localization2
EHD1 fluorescence
Cell SpecificAstrocytes
Function & Pathway6
Relations & Evidence11

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
EHD1EGFRP00533Y453phosphorylationKEAKEA:17570479

Ligand-Receptor Signaling (6)

6 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo
plasma_membraneplasma_membraneUniProt_locationNoNoNoNoNo
plasma_membraneplasma_membraneOmniPathNoNoNoNoNo

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationSize Exclusion ChromatographyMass spectrometryWestern blotting23788664838343172
Sequence, Structure & Domains13

Sequences

Length
534
Mass
60,627
Sequence
MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE

3D Structural Models

Turn
523..525
Helix
440..443; 445..454; 464..473; 478..488; 498..512; 528..530
Beta Strand
458..463; 494..497
3D Structure
NMR spectroscopy (5)

Domain & Motif Annotations

Coiled Coil
198..227
Domain (CC)
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
Domain (FT)
55..286; Dynamin-type G; 444..532; EH; 476..511; EF-hand
Region
65..72; G1 motif; 91..92; G2 motif; 153..156; G3 motif; 219..222; G4 motif; 243; G5 motif
Protein Families (3)
  • TRAFAC class dynamin-like GTPase superfamily
  • Dynamin/Fzo/YdjA family
  • EHD subfamily
Sequence Similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
Clinical Relevance4
Interaction Protein (5)
ENSG00000013016ENSG00000099940ENSG00000100139ENSG00000131381ENSG00000185722
Interaction Count
5
Interaction Dataset (2)
intact_biogridintact_biogrid_bioplex
Supporting Publications113
PMIDTitleAbstract
31065972Proteomic Profiling of Exosomes Derived from Plasma of HIV-Infected Alcohol Drinkers and Cigarette Smokers.No abstract available
31320591Exosomes regulate neurogenesis and circuit assembly.No abstract available
31366407Proteomic and histopathological characterisation of sicca subjects and primary Sjögren's syndrome patients reveals promising tear, saliva and extracellular vesicle disease biomarkers.No abstract available
31508500Proteomic profiling of extracellular vesicles allows for human breast cancer subtyping.No abstract available
31685984Tumour exosomal CEMIP protein promotes cancer cell colonization in brain metastasis.Proteomic analysis identified cell migration-inducing and hyaluronan-binding protein (CEMIP) as elevated in exosomes from brain metastatic but not lung or bone metastatic cells.
31694344Proteomic Analysis of Urinary Extracellular Vesicles Reveals a Role for the Complement System in Medullary Sponge Kidney Disease.No abstract available
32295833Dysregulation of Exosome Cargo by Mutant Tau Expressed in Human-induced Pluripotent Stem Cell (iPSC) Neurons Revealed by Proteomics Analyses.Notably, mTau exosomes (not control exosomes) contain ANP32A (also known as I1PP2A), an endogenous inhibitor of the PP2A phosphatase which regulates the phosphorylation state of p-Tau.
32795414Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers.Among traditional exosome markers, CD9, HSPA8, ALIX, and HSP90AB1 represent pan-EVP markers, while ACTB, MSN, and RAP1B are novel pan-EVP markers.
32927759Urinary Exosomes of Patients with Cystic Fibrosis Unravel CFTR-Related Renal Disease.No abstract available
33017416Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers.No abstract available
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